Adenovirus-mediated gene transfer of dopamine D2 receptor cDNA into rat striatum

Dopamine D2R DNA transfer in dopamine D2 receptor-deficient mice: Effects on ethanol drinking

“…DNA transfer via adenoviral vector has been an effective strategy to introduce particular genes into tissue and has provided a high specificity targeting and delivery (Crystal, 1992;Suhr and Gage, 1993). The effectiveness of the present vector for intracerebral transfer of D2R as well as the expression of functional D2R effects has been previously well established (Ikari et al, 1995(Ikari et al, , 1999Ingram et al, 1998;Ogawa et al, 2000;Thanos et al, 2001;Umegaki et al, 1997). Rats infected with this adenoviral vector produced significant increases in D2R expression with significant decreases in alcohol preference and intake (Thanos et al, 2001(Thanos et al, , 2004.…”

“…Previous reports describe details of the vector construction (Ikari et al, 1995;Umegaki et al, 1997). Briefly, the recombinant Ad vectors were derived from Ad type 5 in which the majority of the E1 and a portion of the E3 regions had been deleted, and an expression cassette containing the rat D2R cDNA along with the cytomegalovirus (CMV) immediate early promotor and enhancer was inserted at the site of the E1 deletion.…”

Aging of the striatum: mechanisms and interventions

“…Details of the vector construction are documented elsewhere (Ikari et al 1995). In brief, the vector was constructed by deletion of the majority of E1 and a portion of E3 region, and insertion of an expression cassette containing the rat D 2 R cDNA along with the cytomegalovirus immediate early promoter and enhancer (AdCMV.DopD 2 R).…”

Sindbis viral-mediated expression of eGFP-dopamine D1 receptors in situ with real-time two-photon microscopic detection

“…There are several unique aspects to this report. While baculovirus has been used to infect insect cells to express D1 receptors as well as other G-protein-coupled receptors (Ng et al, 1994;Parker et al, 1991;Woodcock et al, 1993), and D2 receptors have been expressed in mammalian neurons using an adenovirus (Ikari et al, 1995); this is the first report of D1DR expression via viral infection in mammalian neurons. Additionally, this is the first report of two-photon excitation and real-time analysis of a virally-expressed eGFP-labeled G-protein-coupled receptor in any cell type.…”