Adenovirus Type 8 Epidemic Keratoconjunctivitis in an Eye Clinic: Risk Factors and Control

Jernigan, John A.; Lowry, Barbara S.; Hayden, Frederick G.; Kyger, Shirley A.; Conway, Brian; Gröschel, D; Farr, Barry M.

doi:10.1093/infdis/167.6.1307

Cited by 113 publications

(59 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Most of these diseases heal naturally, but sometimes the infection may also provoke serious illnesses such as pneumonia caused by AdV type 7 (AdV-7) or epidemic keratoconjunctivitis (EKC) due to AdV-8, -19a, or -37. These more serious outcomes occur in all age groups and can possibly trigger highly contagious nosocomial infections (5,6,19,27,42). Therefore, it is important to establish a rapid method of virological diagnosis.…”

mentioning

confidence: 99%

Quantitative Detection and Rapid Identification of Human Adenoviruses

et al. 2007

View full text Add to dashboard Cite

We have established a method of quantitative detection and rapid identification of human adenoviruses (hAdVs). Using LightCycler PCR with a primer set, we were able to amplify 554 bp of the hexon gene from each of 51 prototype strains of hAdVs. The sensitivity of LightCycler PCR was 10 copies of hAdV DNA/reaction. When LightCycler PCR was performed using a set of primers, hAdV was positive for 74.4% (99 of 133) of conjunctivitis patients and for 27.3% (81 of 297) of respiratory infection patients. We also attempted to measure hAdV in the potentially contaminated eye drops used by patients, detecting 5.4 ؋ 10 2 to 1.6 ؋ 10 Human adenoviruses (hAdVs) of the genus Mastadenovirus of the family Adenoviridae infect billions of people worldwide and cause various diseases such as conjunctivitis, respiratory infectious disease, diarrhea in infants and young children, hemorrhagic cystitis, etc. (1,8,33,39,40). Most of these diseases heal naturally, but sometimes the infection may also provoke serious illnesses such as pneumonia caused by AdV type 7 (AdV-7) or epidemic keratoconjunctivitis (EKC) due to . These more serious outcomes occur in all age groups and can possibly trigger highly contagious nosocomial infections (5,6,19,27,42). Therefore, it is important to establish a rapid method of virological diagnosis. Furthermore, hAdV infection in immunosuppressed patients, such as graft recipients and immunodeficient patients including those with AIDS, has been a major problem in recent years and has been lethal in many cases (7,9,10,17,23,40,41). Nosocomial infection is also a serious problem which may require restriction of hospitalization and closing of hospital wards (15). Therefore, it is essential to monitor these viruses, and a rapid method of identifying serotypes is urgently needed.hAdVs were initially grouped into six subgenera (A to F) on the basis of several biochemical and biophysical criteria (1, 39). In 1999, reclassification on the basis of nucleotide and deduced amino acid sequences was approved by the International Committee on Taxonomy of Viruses; under this reclassification, the 51 serotypes of hAdVs in the genus Mastadenovirus were grouped into six species, hAdV-A to hAdV-F (38). Virus isolation followed by a neutralization test has been the standard method of serotyping (39); however, these procedures are complicated and time-consuming, and the standardized antisera are in limited supply. Recent advances in amplifying hAdV genes and decoding nucleotide sequences have allowed us to develop a PCR-restriction fragment length polymorphism method with which we have succeeded in distinguishing 14 hAdVs including AdV-3, -4, -8, -19a, and -37, all of which cause eye infections in humans (31). Furthermore, nucleotide sequence analysis of the partial hexon genes (916 bp) of all 33 prototype strains in hAdV-D and hAdV-E allowed us to construct a database for the phylogeny-based identification of hAdVs from patients with conjunctivitis (34). However, this method requires several overlapping sequences to determine 9...

show abstract

mentioning

confidence: 99%

Quantitative Detection and Rapid Identification of Human Adenoviruses

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Estas lesiones se resuelven en un plazo no inferior a 15 días pudiendo producir depósito de inmunocomplejos subepiteliales a nível de la capa de Bowman, y formando opacidades que provocan aumento de la fotofobia dificultando así la visón. Como resultado de lo anterior, se fomenta un estado incapacitante para el paciente debido a que tardan en reabsorberse hasta un período que puede durar meses e incluso años (7,8,14). Nuestros resultados corroboran lo descrito anteriormente ya que pudo observarse en tres de nuestros casos la formación de pseudomembrana y que la afección a nivel corneal manifestada como queratitis punteada superficial e infiltrados subepiteliales fue una característica más acentuada en los afectados por Ad8 a diferencia del resto de los pacientes afectados por Ad1, Ad2 yEn este estudio se identificaron los serotipos Ad1, Ad2, Ad3 y Ad8 en pacientes con diagnostico clínico de conjuntivitis folicular y queratoconjuntivitis epidémica en el período comprendido de enero de 2005 a diciembre de 2006.…”

Section: Discussionunclassified

“…En la literatura se ha mostrado la variedad de serotipos de Ad pertenecientes a los subgeneros B (Ad3 y Ad7), C (Ad1, Ad2, y Ad5), o E (Ad4), como causantes esporádicos de conjuntivitis, conjuntivitis folicular o fiebre faringo-conjuntival (4)(5)(6). Los brotes epidémicos son producidos por el subgenero D fundamentalmente por Ad8 asociado a queratoconjuntivitis epidémica (7,8).…”

Section: Introductionunclassified

“…Esto implica, que la identificación en muestras oculares de los serotipos Ad1, Ad2 y Ad5 que afectan preferentemente vías respiratorias (subgénero C) es menos grave en el pronóstico de el cuadro oftalmológico, que la detección de serotipos como el Ad8, Ad19 y Ad37 los cuales son los agentes más comunes en la queratoconjuntivitis hemorrágica, una infección ocular altamente contagiosa y discapacitante (7,8).…”

Section: Introductionunclassified

See 1 more Smart Citation

Caracterización genética de adenovirus aislados de conjuntivitis folicular y queratoconjuntivitis epidémica en un grupo de pacientes mexicanos

Mejia-Lopez

Santacruz-Valdes

Matías-Florentino

2008

Arch Soc Esp Oftalmol

View full text Add to dashboard Cite

RESUMENObjetivo: Conocer los serotipos de adenovirus en pacientes mexicanos con conjuntivitis folicular y queratoconjuntivitis. Métodos: Se analizaron por PCR específica para adenovirus, muestras de fondo de saco conjuntival inferior de 77 pacientes diagnosticados con conjuntivitis folicular por sospecha clínica de infección por adenovirus, de enero de 2005 a diciembre de 2006. La identificación de los serotipos se realizó por secuenciación automatizada. Las secuencias de nucleótidos obtenidos fueron comparados con las secuencias reportadas en el GenBank. En el análisis de los resultados se utilizó la estadística descriptiva. Resultados: Se analizaron 77 muestras de las cuales el 56% presentaron adenovirus. La secuenciación de cada muestra positiva permitió la identificación de Ad1, Ad2, Ad3 y Ad8; las secuencias de los serotipos fueron idénticas a las reportadas en el GenBank con las direcciones: AF 534906 y AY 224420 para una secuencia del gen que codifica el filamento de Ad1 y Ad2 respectivamente y AY 854180 y DQ 149614 para una secuencia del gen

show abstract

“…Similarly, the ophthalmologist can act as vector causing transmission of adenovirus particles from patient to patient. 5,6 Previous reports have shown how outbreaks can rapidly develop, 7,8 potentially resulting in temporary closure of ophthalmic units. Their prompt containment is therefore necessary because of their grave implications on the health of the public and workforce.…”

Section: Introductionmentioning

confidence: 99%

Epidemic kerato-conjunctivitis—do outbreaks have to be epidemic?

Cheung

Bremner

Chan

2003

Eye

View full text Add to dashboard Cite

Purpose To study: the epidemiology of an outbreak of adenoviral keratoconjunctivitis in a UK teaching hospital; disease presentation and its effect on clinical diagnostic efficiency; patterns of viral transmission between staff and patients; the effectiveness of infection control procedures in minimising outbreaks.Methods Prospective/retrospective clinical audit and retrospective audit of virological culture results: all viral culture swabs taken during an outbreak of adenoviral keratoconjunctivitis were analysed. The case records of patients whose viral swabs were positive for adenoviral culture were traced.

show abstract

Adenovirus Type 8 Epidemic Keratoconjunctivitis in an Eye Clinic: Risk Factors and Control

Cited by 113 publications

References 14 publications

Quantitative Detection and Rapid Identification of Human Adenoviruses

Quantitative Detection and Rapid Identification of Human Adenoviruses

Caracterización genética de adenovirus aislados de conjuntivitis folicular y queratoconjuntivitis epidémica en un grupo de pacientes mexicanos

Epidemic kerato-conjunctivitis—do outbreaks have to be epidemic?

Contact Info

Product

Resources

About