Adenoviruses from Human Immunodeficiency Virus-Infected Individuals, Including Two Strains That Represent New Candidate Serotypes Ad50 and Ad51 of Species B1 and D, Respectively

Abstract

Adenovirus (Ad) isolates from a large number of human immunodeficiency virus (HIV)-infected individuals were compared serologically and genetically with Ad isolates from immunocompetent patients. Between 1982 and 1994, stool and urine samples from 137 subjects with AIDS hospitalized in The Netherlands yielded 143 Ad strains. Forty additional Ad strains were obtained from 35 HIV-positive patients in Manchester, United Kingdom, in 1992 and 1993. Of these 183 HIV-associated Ad strains, 84% belonged to species D and 3% belonged to species C. These strains were compared with 2,301 Ad strains collected during general diagnostic examinations in The Netherlands from 1973 to 1992. Of the latter strains, 5% belonged to species D and 49% belonged to species C. Two of the Ads isolated from fecal specimens of AIDS patients represent new serotypes: candidate Ad serotype 50 (prototype strain, Wan) of subspecies B1 and candidate Ad serotype 51 (prototype strain, Bom) of species D. The DNA restriction enzyme patterns of strains Wan and Bom differed from the patterns of all established prototypes.

A novel Chimpanzee serotype-based adenoviral vector as delivery tool for cancer vaccines

“…The adenoviruses (Ads) comprise a large family of doublestranded DNA viruses found in amphibians, avians and mammals, which have a nonenveloped icosahedral capsid structure [1][2][3][4]. Ad vectors have been developed for gene therapy purposes with the aim of treating inherited or acquired diseases [5].…”

Molecular Diagnosis of Human Adenoviruses D and E by a Phylogeny-Based Classification Method Using a Partial Hexon Sequence

“…Genome typing has been used for the classification of HAdVs (1,9,16,31,42). This method, based on the cleavage pattern of the full genome of HAdV, is capable of distinguishing the prototype strains of 51 serotypes.…”

“…Human adenoviruses (HAdVs) in the genus Mastadenovirus of the family Adenoviridae form a group of viruses with numerous serotypes (1,9,16,31,38,42). HAdVs infect billions of people worldwide and cause various clinical manifestations, such as keratoconjunctivitis, upper and lower respiratory tract infections, hemorrhagic cystitis, and gastroenteritis (18,21).…”

Emerging Cancer Vaccines: The Promise of Genetic Vectors

“…The adenoviruses comprise a large family of double-stranded DNA viruses found in amphibians, avians and mammals, which have a non-enveloped icosahedral capsid structure [47,48] and genome sizes varying between 36 and 40 kb. In contrast to retroviruses, Ads can transduce dividing and non-dividing cells, without integrating into the genome of the host cell.…”