Adenylate cyclase of rat pituitary gland

Borghi, C.; Nicosia, S.; Giachetti, Antonio; Said, Sami I.

doi:10.1016/0014-5793(79)80573-6

Cited by 29 publications

(5 citation statements)

References 24 publications

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“…vestigators have reported (15) that dopamine failed to inhibit the stimulation of pituitary adenylate cyclase by VIP. A reason for the apparent discrepancy existing between the present results and the report by Borghi et aL (15) might be that they used the whole rat pituitary gland. Because VIP can activate the adenylate cyclase ofposterior pituitary (25), the inhibitory effect ofDA might have been masked by the insensitivity to dopamine inhibition of the VIP-stimulated adenylate cyclase located in posterior pituitary.…”

Section: Methodscontrasting

confidence: 56%

“…This peptide increases the plasma concentration ofprolactin in male (13) or ovariectomized female rats (14). In vitro, VIP activates pituitary adenylate cyclase (15) and stimulates prolactin secretion selectively (16). Thus, the VIP-sensitive adenylate cyclase could be an interesting model to ascertain whether the dopamine recognition sites of mammotrophs are coupled with adenylate cyclase.…”

mentioning

confidence: 99%

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Dopaminergic modulation of adenylate cyclase stimulation by vasoactive intestinal peptide in anterior pituitary.

Onali¹,

Schwartz²,

Costa³

1981

Proc. Natl. Acad. Sci. U.S.A.

105

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Section: Methodscontrasting

confidence: 56%

mentioning

confidence: 99%

Dopaminergic modulation of adenylate cyclase stimulation by vasoactive intestinal peptide in anterior pituitary.

Onali¹,

Schwartz²,

Costa³

1981

Proc. Natl. Acad. Sci. U.S.A.

105

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“…Pituitary VIP receptors are functionally independent from those of other known hypothalamic factors regulating prolactin secretion (Borghi, Nicossia, Giachetti & Said, 1979;Haisenlender, Moy, Gala & Said, 1988) and the density of VIP-binding sites on pituitary membranes has been reported to be modified by prolactin status (Montagne, Vial, Joubert-Breession & Rostene, 1989). The inhibition of prolactin synthesis by dopamine appears to be mediated, at least in part, through inhibition of cyclic AMP (cAMP) production.…”

Section: Discussionmentioning

confidence: 97%

Rat anterior pituitary neuropeptides following chronic prolactin manipulation: a combined radioimmunoassay and mRNA study

O’Halloran

Jones²,

Ghatei³

et al. 1991

Journal of Endocrinology

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Prolactin secretion is highly regulable, and the possibility exists that there are local intrapituitary factors controlling prolactin secretion. Recently, the neuropeptides vasoactive intestinal peptide (VIP), galanin and substance P (SP) have been co-localized to the lactotroph in the female rat. We investigated the effects of alterations in prolactin status in vivo on pituitary and hypothalamic expression of these peptides by specific radioimmunoassays and mRNA analysis. In the anterior pituitary, following haloperidol treatment, the contents of both VIP and galanin were suppressed to below detectable levels. Similarly, after bromocriptine treatment, the content of VIP was decreased to below the detection limit of the assay while galanin (14.2 +/- 1.3 vs control 21.0 +/- 2.1 fmol/mg, P less than 0.05) also showed a significant reduction. The levels of VIP mRNA and galanin mRNA in these groups showed the same qualitative change as their respective peptides. Concurrent treatment with high-dose oestrogen modified the VIP peptide response to bromocriptine (1368.7 +/- 149.2 vs bromocriptine 843.4 +/- 82.7 fmol/mg, P less than 0.05) but not to haloperidol. Oestrogen-induced decreases in galanin content were not influenced by either treatment. The pituitary content of SP showed a fall after oestrogen treatment (1.1 +/- 0.01 vs control 6.4 +/- 0.8 fmol/mg, P less than 0.05) which was not significantly altered by either bromocriptine or haloperidol. Likewise, SP mRNA levels in the pituitary were decreased by 90% following oestrogen treatment. Hypothalamic expression of these peptides did not change with any of the treatments.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…In contrast, aldosterone, progesterone, estradiol, and testosterone have no effect. These results demonstrate that, in normal rat pituitary cells in culture, glucocorticoids at physiological concentrations rapidly inhibit the cAMP production and prolactin release induced by VIP by acting through specific glucocorticoid receptors.Vasoactive intestinal peptide (VIP) (9,10).In the light of both in vivo and in vitro experiments (6, 11), we suggested that corticosteroids could be involved in the regulation of VIP action on the adenohypophysis. We had previously shown that addition of dexamethasone (Dex) for 2 days to cultures of enriched pituitary rat lactotrophs completely abolished the stimulatory effect ofVIP on PRL secretion (6).…”

mentioning

confidence: 99%

“…However, the use of purified anterior pituitary cells [obtained by means ofvelocity sedimentation at unit gravity (6)] from normal rats indicated that, at physiological concentrations (0.1-100 nM), VIP directly stimulated, in a dose-dependent manner, PRL (9,10).…”

mentioning

confidence: 99%

Rapid glucocorticoid inhibition of vasoactive intestinal peptide-induced cyclic AMP accumulation and prolactin release in rat pituitary cells in culture.

Rotsztejn¹,

Dussaillant²,

Nobou³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

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Vasoactive intestinal peptide (VIP) stimulates both adenosine 3',5'-cyclic monophosphate (cAMP) accumulation and prolactin release in normal rat pituitary cells in culture. cAMP accumulation is significant (P < 0.01) at VIP concentrations as low as 1 nM and reaches a maximum with 0.1 ,IM. Addition of dexamethasone as early as 15 min before VIP inhibits VIP stimulation of both cAMP production and PRL secretion. The rapid inhibition is dose-dependent: it appears at doses as low as 0.01 pM and is complete at 1 pM dexamethasone. Increasing concentrations of dexamethasone induce a noncompetitive type of inhibition, as shown by the decrease in Vm.,. with no change in the apparent K.for VIP. Cycloheximide (1 mM) counteracts the inhibitory effect of dexamethasone on VIP-induced cAMP production, which suggests the involvement ofa rapid protein synthesis mechanism. Ru-26988, a specific glucocorticoid devoid of any mineralocorticoid activity and which does not bind to intracellular transcortin-like component, also produces an inhibition of VIP-induced cAMP accumulation. Corticosterone also inhibits VIP-induced cAMP production but at concentrations higher than those ofdexamethasone. In contrast, aldosterone, progesterone, estradiol, and testosterone have no effect. These results demonstrate that, in normal rat pituitary cells in culture, glucocorticoids at physiological concentrations rapidly inhibit the cAMP production and prolactin release induced by VIP by acting through specific glucocorticoid receptors.Vasoactive intestinal peptide (VIP) (9,10).In the light of both in vivo and in vitro experiments (6, 11), we suggested that corticosteroids could be involved in the regulation of VIP action on the adenohypophysis. We had previously shown that addition of dexamethasone (Dex) for 2 days to cultures of enriched pituitary rat lactotrophs completely abolished the stimulatory effect ofVIP on PRL secretion (6). Therefore, we have investigated (a) the involvement of the cAMP system in the biological effect of VIP on PRL release, (b) the kinetics and the stoichiometry of the interaction between VIP and corticosteroids, and (c) the mechanism by which Dex inhibits PRL release at the pituitary level.In the present communication, we present evidence that, in normal rat pituitary cells in culture, Dex at concentrations as low as 1 pM rapidly inhibits both the cAMP accumulation and the PRL release induced by VIP, by acting through specific glucocorticoid receptors. MATERIALS AND METHODSIsolation and Cell Culture. Male Wistar rats (45-60 days old) were used. Fifteen to 20 anterior pituitaries were minced in small pieces and dispersed enzymatically by incubation in 15-20 ml of minimal essential medium with Earle's salts containing 0.1% trypsin (1:250) (GIBCO) and 0.1% fatty acid-free bovine serum albumin (Sigma) for 2 hr at 370C (12) at pH 7.5 in a silicone-treated Bellco spinner flask. Cells were filtered though 100-,um-mesh nylon to remove DNA of broken cells and centrifuged at 400 g for 5 min; the pellet was resuspended, w...

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Adenylate cyclase of rat pituitary gland

Cited by 29 publications

References 24 publications

Dopaminergic modulation of adenylate cyclase stimulation by vasoactive intestinal peptide in anterior pituitary.

Dopaminergic modulation of adenylate cyclase stimulation by vasoactive intestinal peptide in anterior pituitary.

Rat anterior pituitary neuropeptides following chronic prolactin manipulation: a combined radioimmunoassay and mRNA study

Rapid glucocorticoid inhibition of vasoactive intestinal peptide-induced cyclic AMP accumulation and prolactin release in rat pituitary cells in culture.

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