Adenylate cyclases in Physarum polycephalum: Inhibition of a nuclear enzyme by polyamines

Atmar, Valerie J.; Westland, J.A.; García, Gloria Esperanza Castellanos; Kuehn, Glenn D.

doi:10.1016/0006-291x(76)91182-7

Cited by 17 publications

(5 citation statements)

References 27 publications

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“…Perhaps elevated putrescine levels facilitate the increase in the rate of translation observed during Mucor yeast-to-hypha morphogenesis (158). Polyamines are known inhibitors of AC (5) and PDE (49) and have exhibited inhibitory effects on these activities in cell extracts of M. racemosus (154a). Cyclic AMP has been known to repress ODC synthesis in eukaryotic cells (104) and was observed to do so in M. racemosus (105).…”

Section: Rouxiimentioning

confidence: 99%

Mucor dimorphism.

Orlowski¹

1991

Microbiological Reviews

115

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Section: Rouxiimentioning

confidence: 99%

Mucor dimorphism.

Orlowski¹

1991

Microbiological Reviews

115

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“…and extracellular CAMP phosphodiesterases [4] have been demonstrated in this organism, as have an unusual CAMP-inhibited protein kinase [5] and a polyamine-inhibited nuclear adenylate cyclase possibly involved in gene transcription [6]. In the present study, we report an adenylate cyclase from Physarum polycephalum that is activated by its product CAMP.…”

Section: Elsevierjnorth-holland Biomedical Pressmentioning

confidence: 58%

An adenosine‐3′,5′‐monophosphate activated adenylate cyclase in the slime mold Physarum polycephalum

Smith

Mansour

1978

FEBS Letters

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“…Intracellular levels of cAMP are regulated by two enzymes, adenylate cyclase and phosphodiesterase. Previous studies have shown that the synthesis of cAMP may be regulated by PAs (Atmar et al ., 1976; Choi et al ., 1998). In the Δ ChODC mutant, the abundance of cAMP was downregulated by 1.23‐fold (log2) and expression levels of phosphodiesterase were upregulated by 1.393‐fold (log2), and appressorium function was restored when the Δ ChODC mutant was treated with cAMP.…”

Section: Discussionmentioning

confidence: 99%

Ornithine decarboxylase of the fungal pathogen Colletotrichum higginsianum plays an important role in regulating global metabolic pathways and virulence

Yan

Tang

Yuan

et al. 2021

Environmental Microbiology

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Colletotrichum higginsianum is an important fungal pathogen causing anthracnose disease of cruciferous plants. In this study, we characterized a putative orthologue of yeast SPE1 in C. higginsianum, named ChODC. Deletion mutants of ChODC were defective in hyphal and conidial development. Importantly, deletion of ChODC significantly affected appressoriummediated penetration in C. higginsianum. However, polyamines partially restore appressorium function and virulence indicating that loss of ChODC caused significantly decreased virulence by the crosstalk between polyamines and other metabolic pathways. Subsequently, transcriptomic and metabolomic analyses demonstrated that ChODC played an important role in metabolism of various carbon and nitrogen compounds including amino acids, carbohydrates and lipids. Along with these clues, we found deletion of ChODC affected glycogen and lipid metabolism, which were important for conidial storage utilization and functional appressorium formation. Loss of ChODC affected the mTOR signalling pathway via modulation of autophagy. Interestingly, cAMP treatment restored functional appressoria to the ΔChODC mutant, and rapamycin treatment also stimulated formation of functional appressoria in the ΔChODC mutant. Overall, ChODC was associated with the polyamine biosynthesis pathway, as a mediator of cAMP and mTOR signalling pathways to regulate appressorium function. Our study provides evidence of a link between ChODC and the cAMP signalling pathway and defines a novel mechanism by which ChODC regulates infection-associated autophagy and plant infection by fungi.

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Adenylate cyclases in Physarum polycephalum: Inhibition of a nuclear enzyme by polyamines

Cited by 17 publications

References 27 publications

Mucor dimorphism.

Mucor dimorphism.

An adenosine‐3′,5′‐monophosphate activated adenylate cyclase in the slime mold Physarum polycephalum

Ornithine decarboxylase of the fungal pathogen Colletotrichum higginsianum plays an important role in regulating global metabolic pathways and virulence

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