Adenylate kinase isoenzymes in<i>Aspergillus nidulans</i>

Hoang, Van-Quyen; Jaklitsch, Walter M.; Scrutton, Michael C.

doi:10.1111/j.1574-6968.1991.tb04431.x

Cited by 2 publications

(1 citation statement)

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“…When an intense demand for ATP occurs, the cell lowers the ADP concentration, acquiring ATP by the action of adenylate kinase, which was strongly up-regulated. Although this enzyme shows no evidence of being secreted, it has been reported as exosomal and was found in extracellular extracts of A. nidulans grown in glucose . From this group of enzymes, those lacking evidence of secretion presented total spectral counts higher than 59.…”

Section: Discussionmentioning

confidence: 85%

Secretomic Insight into Glucose Metabolism of Aspergillus brasiliensis in Solid-State Fermentation

Volke-Sepúlveda

Salgado-Bautista

Bergmann³

et al. 2016

J. Proteome Res.

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The genus Aspergillus is ubiquitous in nature and includes various species extensively exploited industrially due to their ability to produce and secrete a variety of enzymes and metabolites. Most processes are performed in submerged fermentation (SmF); however, solid-state fermentation (SSF) offers several advantages, including lower catabolite repression and substrate inhibition and higher productivity and stability of the enzymes produced. This study aimed to explain the improved metabolic behavior of A. brasiliensis ATCC9642 in SSF at high glucose concentrations through a proteomic approach. Online respirometric analysis provided reproducible samples for secretomic studies when the maximum CO production rate occurred, ensuring consistent physiological states. Extracellular extracts from SSF cultures were treated by SDS-PAGE, digested with trypsin, and analyzed by LC-MS/MS. Of 531 sequences identified, 207 proteins were analyzed. Twenty-five were identified as the most abundant unregulated proteins; 87 were found to be up-regulated and 95 were down-regulated with increasing glucose concentration. Of the regulated proteins, 120 were enzymes, most involved in the metabolism of carbohydrates (51), amino acids (23), and nucleotides (9). This study shows the high protein secretory activity of A. brasiliensis under SSF conditions. High glucose concentration favors catabolic activities, while some stress-related proteins and those involved in proteolysis are down-regulated.

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Section: Discussionmentioning

confidence: 85%