Adenylate kinase of nuclei and its distribution in several subcellular fractions of neoplastic tissue

An Improved Method for the Isolation of Rat Liver Nuclei by Density Centrifugation

“…This method involves extensive centrifugation of an homogenate of a fasted rat's liver in 2.2 ~ sucrose, in which nuclei migrate to the bottom of the tube and all other cell components migrate upwards. It has been used by several other workers either unmodified (2,3) or for purification of crude nuclear fractions isolated in isotonic sucrose (4)(5)(6)(7)(8)(9) and has ap-parently given satisfactory results. Unfortunately, at least with the animals available in this laboratory, this procedure often yields extensively agglutinated and contaminated nuclei, especially without prior starvation of the rat.…”