Adequacy of methodology for conducting germination test in forage pea seeds

Machado, Carla Gomes; Martins, Cibele Chalita; Silva, Givanildo Zildo da; Cruz, Simério Carlos Silva; Assis, Raissa Macedo

doi:10.14393/bj-v35n2a20198-41747

Cited by 3 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was also used to evaluate germination of citrus rootstock cultivars (SARMIENTO et al, 2016). The best germination performance in paper substrate can be attributed to the vegetative structure's development lower physical impairment and higher aeration of this substrate in comparison to sand (MACHADO et al, 2019). Use of sand causes difficulties in maintaining the sterile laboratory environment, in addition to difficulties in obtaining, storing, disposing and reusing it, recommendations described in the BRASiL (2009).…”

Section: Resultsmentioning

confidence: 99%

Morphology of seeds and seedlings, and substrates in germination of Citrus limetta

et al. 2022

Self Cite

View full text Add to dashboard Cite

This research studied the morphology of seeds and seedlings, in addition to obtaining information about the type and time of germination of sweet lemon seeds in five substrates (on blotting paper, between blotting paper, on washed sterilized sand, between washed sterilized sand and in paper roll). C. limetta seeds were measured, and external description was performed. Afterwards, sown in the sand and kept at 25 ºC to monitoring germination and morphological description of the resulting seedlings. A second experiment was evaluated for substrate efficiency: paper roll, on and between paper, on and between sand in the germination of C. limetta seeds. Speed index, average time and relative frequency of germination were evaluated. In addition, date of the first and last germination count was established. Treatment averages were compared using the Tukey test at 5% probability. C. limetta seeds are ovoid, slightly wrinkled and polyembryonic. Germination is hypogeal, cryptocotylar or phanerocotylar. The seedlings have leathery eophylls with simple leaves, elliptical shape or close to the elliptical, tending to ovate. It has a pivoting root system that is colored yellow to cream with the presence of secondary roots. The average germination time is between 18 and 22 days. The substrate indicated for seed germination is on paper.

show abstract

Section: Resultsmentioning

confidence: 99%

Morphology of seeds and seedlings, and substrates in germination of Citrus limetta

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The counts were performed daily until 14 days after sowing, when the percentage of seedling emergence in the field was calculated according to Nakagawa (1994). Emergence first count of seedlings in the field: emerged seedlings were counted when they represented 50 ± 1 % of the final emergence (MACHADO et al, 2019). Emergence speed index of seedlings in the field: calculated from the data of daily counts of emerged seedlings in the emergence test and determined using the formula proposed by Maguire (1962).…”

Section: Methodsmentioning

confidence: 99%

“…Germination: conducted on a paper roll substrate, moistened with a water volume equivalent to 2.5 times the weight of the dry substrate, and placed in a biochemical oxygen demand germination chamber at 20 °C. The percentage of normal and abnormal seedlings was determined on the seventh day after sowing (MACHADO et al, 2019). To maintain substrate moisture, the rolls with the seeds were placed in plastic bags (COIMBRA et al 2007).…”

Section: Methodsmentioning

confidence: 99%

Accelerated aging test for forage pea seeds

Machado

Silva

Oliveira

et al. 2019

SCA

Self Cite

View full text Add to dashboard Cite

Forage pea is an important annual, cold climate crop with high levels of essential amino acids in its seeds, which are lacking in the composition of cereal grains. Therefore, livestock farmers use its seeds as a nutritious source of forage. To plant fields of this forage, it is necessary to know the quality of the seeds, determined through germination and vigor tests. In the literature, studies on vigor tests for forage pea seeds are scarce. This study aimed to verify the efficiency of vigor tests, with emphasis on accelerated aging to evaluate the physiological quality of forage pea seeds in order to select lots for seedling emergence in the field. Using accelerated aging tests, 10 lots of forage pea were evaluated for moisture content, thousand-seed weight, emergence percentage, emergence first count, speed index of seedlings in the field, germination, germination first count, and electrical conductivity. The following variations of methodology were used: temperatures of 41 and 42 °C, exposure times of 48 and 72 h, and different relative humidity values in the aging atmosphere, using distilled water (100 % RH), saturated solution of potassium chloride (87 % RH), and sodium chloride (76 % RH). The tests of germination, emergence first count of seedlings in the field, emergence speed index, and electrical conductivity are promising to evaluate the vigor of forage pea seed lots. The accelerated aging test conducted in a solution saturated with potassium chloride at 42 °C for 48 h is adequate to estimate the vigor of forage pea seed lots, providing information similar to that of the emergence of seedlings in the field.

show abstract

“…Similarly, a study by R. C. da, Grzybowski and Panobianco (2016) with maize seeds reported that, depending on the substrate, higher and lower vigor lots could show similar performances, proving that the substrate has a greater effect on the results than the physiological quality of the seeds in germination and seedling vigor tests. However, there have also been reports that high vigor fodder pea and safflower lots were less demanding on substrate conditions than low vigor lots (Gama et al, 2019;Machado, Martins, Silva Cruz, Assis, 2019b).…”

Section: Germination and First Count Testmentioning

confidence: 99%

Envelope method for the germination test of Mombasa grass

Jeromini¹,

Botassini²,

Barros³

et al. 2021

Semina: Ciênc. Agrár.

Self Cite

View full text Add to dashboard Cite

The Rules for Seed Analysis reports the use of a paper envelope to allow use of the germination test with small seeds but does not provide any method or use recommendation. As Mombasa grass has high quality and commercial importance, the objective of this study was to evaluate the viability of the envelope method in the germination test of Mombasa grass seeds, with the aim of achieving greater result accuracy and improvement of seed quality analysis processes in laboratories. The germination of eight lots of Mombasa grass seeds was evaluated with two sowing methods (on paper and in paper envelope). The moisture content and the first germination count were also determined. The experiment was conducted with a completely randomized design. The data were analyzed in a 2 x 8 factor scheme (sowing methods x lots), with four repetitions. The data were subjected to analysis of variance using the F test and when significant, treatment means were compared using the Tukey’s test at 5% probability. Results showed that the envelope method is efficient for the germination test of Mombasa grass seeds and can be easily used by seed analysis laboratories, as it present results equivalent or superior to those obtained using the traditional method on paper, while occupying only 2/5 of the germination chamber space used in the conventional method.

show abstract

Adequacy of methodology for conducting germination test in forage pea seeds

Cited by 3 publications

References 11 publications

Morphology of seeds and seedlings, and substrates in germination of Citrus limetta

Morphology of seeds and seedlings, and substrates in germination of Citrus limetta

Accelerated aging test for forage pea seeds

Envelope method for the germination test of Mombasa grass

Contact Info

Product

Resources

About