Adequacy of the tetrazolium test to evaluate the viability of Jatropha curcas L. seeds

Abstract

The tetrazolium test has been recommended for evaluating the viability of seeds with slow and ununiform germination, especially agroforestry species. The aim of this study was to propose a methodology to evaluate the viability of J. curcas L. seeds using the tetrazolium test and to generate visual aids to support the interpretation of the test. In the first trial, methods of seed preparation and staining were evaluated. The most promising methodologies were used to conduct the second test, which consisted of the evaluation of different concentrations (0.10 and 0.25%) and times (2 and 3 h) of immersion of the seeds in the tetrazolium solution, using five lots. The seeds were analyzed individually and classified as viable or non-viable. At the same time, germination, first count, emergence and seedling emergence speed index tests were conducted. The tetrazolium test is efficient for evaluating the viability of seeds of J. curcas L. providing results correlated with germination and seedling emergence. Pre-soaked seeds should have their integument removed and then sectioned longitudinally and medially lengthwise for immersion in 0.10% solution of 2,3,5-triphenyl chloride tetrazolium for three hours at 35 °C.

How to access viability of Zanthoxylum rhoifolium seeds? A protocol of tetrazolium test as an alternative to evaluate a dormant seed

“…In forest seeds, the tetrazolium test has proven its effectiveness, as in Araucaria angustifolia (Oliveira et al, 2014), Libidibia ferrea (Carvalho et al, 2017) Jatropha curcas (Araújo et al, 2019) and Campomanesia phaea (Silva et al, 2021). However, there is still a need for appropriate methodologies sorted by species, especially for native ones (Oliveira et al, 2018) like Z. rhoifolium, which are known especially by the difficulty in promoting their germination.…”

Assessment of the tolerance of soybean seeds to weathering deterioration in the pre-harvest phase by multivariate analysis

“…PCA has been widely used in seed research work due to its high efficiency in identifying relationships between variables . This analysis proved to be effective in studies on the adequacy of methodology for analyzing the viability of Jatropha curcas seeds by the tetrazolium test (Araújo et al, 2019), discrimination of sunflower seed lots with different deterioration levels (Morais et al, 2021), assessment of the physiological quality of wheat seeds produced under different irrigation depths and leaf silicon levels , and assessment of the effect of osmotic conditioning in sunflower seeds (Barros et al, 2021), among others. In addition, it has been adopted as a strategy in the pre-processing of data used in machine learning (Fan et al, 2020;Medeiros et al, 2020;Shi et al, 2020) and exploratory data analysis (Ribeiro et al, 2021;Wang et al, 2021).…”

TETRAZOLIUM AND INTERACTION OF TEMPERATURE AND LIGHT UNDER SEED GERMINATION IN Ormosia arborea (FABACEAE)

“…In this sense, some researches had been carried out with forest species defining the methodology for conducting the tetrazolium test, such as in Leucaena leucocephala (Lam.) (Costa and Santos, 2010), Helianthus annuus (Silva et al, 2013), Plinia cauliflora (Hössel et al, 2013), Acca sellowiana (Sarmento et al, 2018), Eugenia brasiliensis, Eugenia pyriformis (Lamarca and Barbedo, 2014), Tabebuia roseoalba (Abbade and Takaki, 2014), Eugenia uniflora (Kaiser et al, 2014), Apuleia moralis (Reis et al, 2016) and Jatropha curcas (Araújo et al, 2019).…”