Adherence and kinetics of biofilm formation of Staphylococcus epidermidis to different types of intraocular lenses under dynamic flow conditions

Baillif, S.; Écochard, René; Casoli, E; Freney, J.; Burillon, C.; Kodjikian, Laurent

doi:10.1016/j.jcrs.2007.07.058

Cited by 32 publications

(28 citation statements)

References 57 publications

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“…S. epidermidis adhered to a higher extent to the hydrophobic acrylic polymer than to PMMA or the hydrophilic acrylic biomaterial. These results suggest that hydrophobic IOLs are more permissive to S. epidermidis adhesion and growth than hydrophilic IOLs, which is in accordance with previous published data [10,11,12,15]. …”

Section: Discussionsupporting

confidence: 93%

“…To overcome these problems, we have designed an in vitro model that more closely resembled intraocular conditions [14]. This model allowed the study of bacterial biofilm formation (from the primary attachment phase to the biofilm maturation phase) on IOLs [15]. Our first results, using a Staphylococcus epidermidis strain, suggested that hydrophobic IOLs were more permissive to bacterial adhesion and growth than hydrophilic IOLs [15].…”

Section: Introductionmentioning

confidence: 99%

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Staphylococcus epidermidis Biofilm Formation and Structural Organization on Different Types of Intraocular Lenses under in vitro Flow Conditions

2013

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Aim: To compare the adherence and structural organization of Staphylococcus epidermidis biofilm on intraocular lenses (IOLs). Methods: IOLs made of 3 different biomaterials [polymethyl methacrylate (PMMA), hydrophilic acrylic or hydrophobic acrylic] were incubated into an S. epidermidis bacterial solution. Scanning electron microscopy was used to count the bound bacteria and to analyze the structural biofilm architecture. Results: After 4-6 h of incubation, adherence was statistically weakest on the hydrophilic acrylic polymer. On the hydrophobic acrylic material, the bacterial cells tended to cover the substratum in a horizontal spread in a continuous monolayer. On the hydrophilic acrylic material or on the PMMA material bacterial cells tended to form only few, small scattered cell clusters. Conclusions: The data suggest that the pattern of S. epidermidis adhesion varies with the IOL biomaterial. Hydrophobic IOLs seem to be more permissive to S. epidermidis adhesion.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Staphylococcus epidermidis Biofilm Formation and Structural Organization on Different Types of Intraocular Lenses under in vitro Flow Conditions

2013

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“…Reducing the number of organisms present on the ocular surface with the preoperative application of topical povidone iodine in the conjunctival sac and periocular skin is the only measure with proven prophylactic value against endophthalmitis (11,12). Endophthalmitis is a potentially sight-threatening infection (13). Detection of endophthalmitis and identification of the causative agent are therefore essential to ensure that rational treatment is given (14), yet routine microbiologic diagnosis relies on time consuming classical culture techniques that offer low sensitivity; indeed, the positivity rates range from 22% to 30% in the aqueous humor and from 40% to 69% in the vitreous humor (15).…”

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confidence: 99%

Analysis of Intraocular Lens Biofilms and Fluids After Long-Term Uncomplicated Cataract Surgery

Mazoteras

Quiles

Bispo

et al. 2016

American Journal of Ophthalmology

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“…In ophthalmology, many authors have observed that IOLs, described as being hydrophobic, were more permissive to bacterial adhesion and growth than IOLs described as being hydrophilic [24,25]. Clear-cut relations between bacterial adhesion and IOL surface hydrophobicity are not obtained through these studies.…”

Section: Discussionmentioning

confidence: 94%

Calculation of Intraocular Lens Surface Free Energy and its Components from Contact Angle Measurements

et al. 2013

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One of the most important biomaterial characteristics involved in bacterial adhesion on intraocular lenses (IOLs) is hydrophobicity. We calculated the hydrophobicity parameters of IOLs made of 6 different materials (polymethylmethacrylate, PMMA, heparin surface-modified PMMA, HSM-PMMA, silicone, hydrophilic and hydrophobic acrylics and collamer). Values of IOL surface free energy components were determined from contact angle measurements, using the Fowkes, Owens-Wendt and Good-van Oss calculations. Contact angles were higher for silicone and hydrophobic acrylic materials and lower for collamer and hydrophilic acrylic materials. The values of IOL surface free energy components obtained with the 3 different calculations were homogenous. According to the Owens-Wendt calculation, the IOLs could be separated into dispersive implants (hydrophobic acrylic, silicone and PMMA) and polar implants (collamer, hydrophilic acrylic and HSM-PMMA).

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Adherence and kinetics of biofilm formation of Staphylococcus epidermidis to different types of intraocular lenses under dynamic flow conditions

Abstract: Bacterial adhesion to and biofilm development on the IOL surface depended on the characteristics of the biomaterial.

Cited by 32 publications

References 57 publications

<b><i>Staphylococcus epidermidis</i></b> Biofilm Formation and Structural Organization on Different Types of Intraocular Lenses under in vitro Flow Conditions

<b><i>Staphylococcus epidermidis</i></b> Biofilm Formation and Structural Organization on Different Types of Intraocular Lenses under in vitro Flow Conditions

Analysis of Intraocular Lens Biofilms and Fluids After Long-Term Uncomplicated Cataract Surgery

Calculation of Intraocular Lens Surface Free Energy and its Components from Contact Angle Measurements

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