Adherence &lt;i&gt;in vitro&lt;/i&gt; of &lt;i&gt;Neisseria gonorrhoeae, Escherichia coli&lt;/i&gt; and Group B Streptococci to Vaginal Epithelial Cells of Post-Menopausal Women

Forslin, Lars; Danielsson, Dan; Falk, Viking

doi:10.1159/000299855

Cited by 12 publications

(6 citation statements)

References 15 publications

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“…gonorrhoeae was isolated from the vaginal aspirates of 17 (85%) of the 20 patients with gonorrhoea in this study. Although gonococci have been shown to adhere to vaginal epithelial cells in vitro Forslin, Danielsson and Falk, 1980), gonococci aspirated from the vaginal pool are probably derived from the cervix, the site of multiplication of the organisms (Ward, Watt and Robertson, 1974). The mean viable count of gonococci in vaginal aspirates (8.4 x lo4 cfulml) was considerably lower than that in cervical aspirates (1 x 1 O6 cfu/ml) although the difference did not attain statistical significance (p > 0.05).…”

Section: Discussionmentioning

confidence: 91%

Non-cultural detection of Neisseria gonorrhoeae in cervical and vaginal washings

Young

Sarafian

Harris

et al. 1983

Journal of Medical Microbiology

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SUMMARY. Genetic transformation, an indirect sandwich enzymelinked immunosorbent assay (ELISA) and the Limulus amoebocyte assay were used to indicate the presence of products of Neisseria gonorrhoeae in vaginal and uterine cervical aspirates from 37 women attending a Department of Genito-Urinary Medicine. In parallel with these tests, qualitative and quantitative assessments of the microbial content of aspirates were made. There was wide variation in the numbers of gonococci cultured. The mean viable count for cervical aspirates was 1 x 1 O6 cfu/ml and the range was (5 x 103)--(8 x lo6) cfu/ml; the mean count for vaginal aspirates was 8.4 x lo4 cfu/ml and the range (1 x 102)-(1 x lo6) cfu/ml. Viable counts of organisms other than gonococci in vaginal aspirates were two to tenfold greater than the corresponding counts for cervical aspirates. Of 20 patients with gonorrhoea confirmed by conventional diagnostic cultures, aspirates from 15 (75%) gave a positive transformation result, and 12 (60%) a positive ELISA result; 16 (84.2%) out of 19 of these aspirates tested by the Limulus lysate assay were positive at a dilution of 1 in 100.

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Section: Discussionmentioning

confidence: 91%

Non-cultural detection of Neisseria gonorrhoeae in cervical and vaginal washings

Young

Sarafian

Harris

et al. 1983

Journal of Medical Microbiology

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“…Estrogen treatment increases the bacterial adherence capacity of rat bladders (501) and HeLa cells (511). However, whether hormonal fluxes account for cyclic changes in bacterial adherence capacity in women is uncertain, since there is no apparent increase in adherence capacity associated with the use of oral contraceptives (475) or with estrogen replacement therapy after menopause (132). Increasing age is not consistently associated with an increased epithelial-cell adherence capacity (440,475,502).…”

Section: Host-pathogen Interactions In Adherencementioning

confidence: 99%

Virulence factors in Escherichia coli urinary tract infection

1991

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Uropathogenic strains of Escherichia coli are characterized by the expression of distinctive bacterial properties, products, or structures referred to as virulence factors because they help the organism overcome host defenses and colonize or invade the urinary tract. Virulence factors of recognized importance in the pathogenesis of urinary tract infection (UTI) include adhesins (P fimbriae, certain other mannose-resistant adhesins, and type 1 fimbriae), the aerobactin system, hemolysin, K capsule, and resistance to serum killing. This review summarizes the virtual explosion of information regarding the epidemiology, biochemistry, mechanisms of action, and genetic basis of these urovirulence factors that has occurred in the past decade and identifies areas in need of further study. Virulence factor expression is more common among certain genetically related groups of E. coli which constitute virulent clones within the larger E. coli population. In general, the more virulence factors a strain expresses, the more severe an infection it is able to cause. Certain virulence factors specifically favor the development of pyelonephritis, others favor cystitis, and others favor asymptomatic bacteriuria. The currently defined virulence factors clearly contribute to the virulence of wild-type strains but are usually insufficient in themselves to transform an avirulent organism into a pathogen, demonstrating that other as-yet-undefined virulence properties await discovery. Virulence factor testing is a useful epidemiological and research tool but as yet has no defined clinical role. Immunological and biochemical anti-virulence factor interventions are effective in animal models of UTI and hold promise for the prevention of UTI in humans.

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“…The highest levels of adherence were consistently obtained with E. coli 100, which caused MS hemagglutination of guinea pig erythrocytes. (19), pelvic inflammatory disease induced by Neisseria gonorrhoeae (5,6), and experimental endometritis caused by E. coli (12). Predisposition to infection by different organisms appears to be affected by differing hormonal influences.…”

Section: Resultsmentioning

confidence: 99%

Enhancement of Escherichia coli adherence to epithelial cells derived from estrogen-stimulated rats

Sobel¹,

Kaye²

1986

Infect Immun

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The effect of exogenous estrogen administered to male and oophorectomized female rats was investigated with regard to in vitro adherence of eight uropathogenic strains of Escherichia coli to exfoliated bladder and vaginal epithelial cells. Uroepithelial cells obtained from estrogenized male and estrogenized oophorectomized female rats and vaginal cells obtained from estrogenized oophorectomized female rats demonstrated significantly enhanced (P less than 0.005) host cell avidity for E. coli attachment, irrespective of bacterial adhesin expressed, when compared with such cells from nonestrogenized male and female oophorectomized rats. These animal studies suggest that female reproductive hormones may contribute to urinary-tract infection in premenopausal females by enhancing susceptibility to E. coli colonization of uroepithelial cells.

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Adherence <i>in vitro</i> of <i>Neisseria gonorrhoeae, Escherichia coli</i> and Group B Streptococci to Vaginal Epithelial Cells of Post-Menopausal Women

Cited by 12 publications

References 15 publications

Non-cultural detection of Neisseria gonorrhoeae in cervical and vaginal washings

Non-cultural detection of Neisseria gonorrhoeae in cervical and vaginal washings

Virulence factors in Escherichia coli urinary tract infection

Enhancement of Escherichia coli adherence to epithelial cells derived from estrogen-stimulated rats

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