Adherence of Acinetobacter baumannii strains to human bronchial epithelial cells

Lee, Je Chul; Koerten, Henk K.; Broek, Peterhans van den; Beekhuizen, Henry; Wolterbeek, Ron; Barselaar, Maria van den; Reijden, Tanny van der; Meer, Joke van der; Gevel, Joke van de; Dijkshoorn, Lenie

doi:10.1016/j.resmic.2005.09.011

Cited by 134 publications

(108 citation statements)

References 31 publications

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“…The protein equivalent to CsuE of Vibrio parahaemolyticus, a chaperone has been identified as a key factor in pilus and biofilm formation in a pioneer study (Tomoras et al, 2004). Surprisingly, considerable variation in quantitative adherence was observed among different strains of A. baumannii isolated from the same geographical region (Lee et al, 2006). This observation of varying degree of adherence among the strains is in concordance with our studies (unpublished data).…”

Section: Biofilm Development Mechanisms In a Baumanniisupporting

confidence: 92%

“…A. baumannii has the ability to colonize both abiotic and medical devices (Tomoras et al, 2003) and form biofilms that display decreased susceptibility to multiple antibiotics (Uma Karthika et al, 2008). Adherence of A. baumannii to human bronchial epithelial cells and erythrocytes has already been demonstrated, with pilus like structures appear to be important for adherence (Gospodarek et al, 1998, Lee et al, 2006. This process is considered to be a first step in the colonization process of A. baumannii.…”

Section: Biofilm Development Mechanisms In a Baumanniimentioning

confidence: 95%

See 1 more Smart Citation

Antibiotic Resistance, Biofilms and Quorum Sensing in Acinetobacter Species

Prashanth¹,

Vasanth²,

Saranathan³

et al. 2012

Antibiotic Resistant Bacteria - A Continuous Challenge in the New Millennium

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Section: Biofilm Development Mechanisms In a Baumanniisupporting

confidence: 92%

Section: Biofilm Development Mechanisms In a Baumanniimentioning

confidence: 95%

Antibiotic Resistance, Biofilms and Quorum Sensing in Acinetobacter Species

Prashanth¹,

Vasanth²,

Saranathan³

et al. 2012

Antibiotic Resistant Bacteria - A Continuous Challenge in the New Millennium

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“…medical devices and environmental surfaces (3)(4)(5)(6); (ii) its ability to adhere to, colonize and invade human epithelial cells (7,8); (iii) its repertoire of antibiotic resistance mechanisms that are able to be promptly up-regulated as required; and (iv) its ability to acquire foreign genetic material through lateral gene transfer to promote its own survival under antibiotic and host selection pressures (9,10). Specific virulence mechanisms identified to date are summarized in Table 1.…”

Section: Introductionmentioning

confidence: 99%

Insights into Acinetobacter baumannii pathogenicity

2011

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SummaryAcinetobacter spp. have justifiably received significant attention from the public, scientific, and medical communities. Over recent years, Acinetobacter, particularly Acinetobacter baumannii, has become a ''red-alert'' human pathogen, primarily because of its exceptional ability to develop resistance to all currently available antibiotics. This characteristic is compounded by its unique abilities to survive in a diverse range of environments, including those within healthcare institutions, leading to problematic outbreaks. Historically, the virulence of the organism has been questioned, but recent clinical reports suggest that Acinetobacter can cause serious, life-threatening infections. Furthermore, its metabolic adaptability gives it a selective advantage in harsh hospital environments. This review focuses on current understanding of A. baumannii pathogenesis and the model systems used to study this interesting organism.

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“…Our knowledge regarding the interaction between A. baumannii and epithelial cells is very limited. Investigations by Lee et al found that A. baumannii is able to adhere to human bronchial epithelial cells (3). These studies were advanced further as this group studied the innate immune response in human epithelial cells challenged with A. baumannii outer membrane protein A (AbOmpA) (4) and found that while the levels of Toll-like receptor 2 (TLR-2) and inducible nitric oxide synthase (iNOS) were elevated, no change in proinflammatory cytokines/chemokine levels was evident.…”

mentioning

confidence: 99%

Epithelial Innate Immune Response to Acinetobacter baumannii Challenge

et al. 2014

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dCurrently, Acinetobacter baumannii is recognized as one of the major pathogens seriously threatening our health care delivery system. Aspects of the innate immune response to A. baumannii infection are not yet well understood. Human ␤-defensins (hBDs) are epithelial cell-derived cationic antimicrobial peptides (AMPs) that also function to bridge the innate and adaptive immune system. We tested the induction of hBD-2 and -3 by A. baumannii on primary oral and skin epithelial cells and found that A. baumannii induces hBD-3 transcripts to a greater extent than it induces hBD-2 transcripts on both types of cells. In addition, we found that A. baumannii is susceptible to hBD-2 and -3 killing at submicromolar concentrations. Moreover, hBD-3 induction by A. baumannii was found to be dependent on epidermal growth factor receptor (EGFR) signaling. Inhibition of mitogen-activated protein kinase resulted in reduced expression of both hBD-2 and -3. Lastly, a disintegrin and metalloprotease 17 (ADAM17; also known as TACE) was found to be critical for hBD-3 induction, while ADAM10 and dual oxidase 1 (Duox1) were not required for hBD-3 induction. Induction of AMPs is an important component of innate sensing of pathogens and may play an important role in triggering systemic immune responses to A. baumannii infection. Further studies on the interactions between epithelial cells and A. baumannii will help us understand early stages of infection and may shed light on why some individuals are more vulnerable to A. baumannii infection.

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Adherence of Acinetobacter baumannii strains to human bronchial epithelial cells

Cited by 134 publications

References 31 publications

Antibiotic Resistance, Biofilms and Quorum Sensing in Acinetobacter Species

Antibiotic Resistance, Biofilms and Quorum Sensing in Acinetobacter Species

Insights into Acinetobacter baumannii pathogenicity

Epithelial Innate Immune Response to Acinetobacter baumannii Challenge

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