Adherence of an Oral spirochete Treponema denticola to glass surfaces in vitro.

Nakatani, Yoshihiro; Umemoto, Toshihiko; Nakamura, Yoshinori; Noda, Mitsuhiro; Ito, Uichi; Namikawa, Isamu

doi:10.2330/joralbiosci1965.34.374

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…The dilution of the rabbit anitiserum against a surface 53 kDa protein of T denticola ATCC 33520 exhibited decreased adherence of this strain to the antisera-coated glass cover-slips in a similar adherence assay (19), suggesting that surface proteins, including a 53 kDa protein as a CBP, involve specific spirochetal adherence. In order to confirm specific treponemal adherence by the CBPs detected in this study, a more sophisticated adherence inhibiton experiment may be required.…”

Section: Discussionmentioning

confidence: 78%

Adherence of Human Oral Spirochetes by Collagen‐Binding Proteins

Umemoto

Li²,

Namikawa

1997

Microbiology and Immunology

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The ability of spirochetes to adhere to collagens was compared among three species of human oral treponemes. Immunoblot analysis demonstrated that type I-, IV-, and V-collagen-binding polypeptides (CBPs) were detected in the heated and unheated preparations from both Treponema denticola ATCC 33520 and T socranskii subsp. buccale ATCC 35534. Few CBPs, however, were detected in the heated and unheated preparations from a recently characterized isolate, T medium strain G7201. Immunoelectron microscopy using rabbit antisera against the CBPs from the unheated preparations demonstrated that four CBPs, a 27 kDa type V-CBP of T denticola ATCC 33520, a 95 kDa type IV-CBP and a 110 kDa type I-CBP of T socranskii subsp. buccale ATCC 35534, and a 95 kDa type IV-CBP of T medium strain G7201, were located on the outer envelopes of the individual cells. The adherence of T denticola to the collagen-coated surfaces was significantly greater than that of T medium, suggesting that the CBPs on the oral spirochetal cells play an important role in their adherence to collagen-rich connective tissues of the host.

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Section: Discussionmentioning

confidence: 78%

Adherence of Human Oral Spirochetes by Collagen‐Binding Proteins

Umemoto

Li²,

Namikawa

1997

Microbiology and Immunology

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“…Fibronectin can bind to gram-negative or gram-positive microorganisms and mediates their cytoadherence (6,9,11). Treponema pallidum, a causative pathogen of syphilis, and a human oral spirochete T. denticola in subgingival plaques, also bind to the fibronectin (1 -3 , 9 , 11).…”

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confidence: 99%

Fibronectin‐Binding Proteins of a Human Oral Spirochete Treponema denticola

Umemoto

Nakatani

Nakamura

et al. 1993

Microbiology and Immunology

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Major polypeptides from a human oral spirochete Treponema denticola ATCC 33520 were examined to demonstrate their ability to bind to human plasma fibronectin by immunoblot analysis. Of three main polypeptides separated on sodium dodecyl sulfate polyacrylamide gels 53,000-daltons (53-kDa) and 72-kDa surface antigenic proteins and a 38-kDa axial flagellar protein showed the ability to bind to fibronectin, suggesting that fibronectin on host cells can mediate cytoadherence of T. denticola by its binding to the surface proteins or the exposed 38-kDa axial flageller protein.

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Adherence of an Oral spirochete Treponema denticola to glass surfaces in vitro.

Cited by 2 publications

References 22 publications

Adherence of Human Oral Spirochetes by Collagen‐Binding Proteins

Adherence of Human Oral Spirochetes by Collagen‐Binding Proteins

Fibronectin‐Binding Proteins of a Human Oral Spirochete Treponema denticola

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