Adherence of Coagulase-Negative Staphylococci onto Polyethylene Catheters in vitro and in vivo: A Study on the Influence of various Plasma Proteins

Abstract

Bacterial adherence on PE catheters inserted into the carotid artery of rabbits was assessed at 5, 30, and 240 min after injection with bacteria of five coagulase-negative staphylococci (CN Staph). These studies revealed that CN Staph adhere onto PE catheters 5 min after injection with bacteria. At 240 min after injection with bacteria almost all catheters were sterile, indicating that initially adherent bacteria on PE catheters disappear with time. EM studies revealed high numbers of platelets and leukocytes and many fibrin deposits on the surface of the PE catheter. In addition, the adherence of the five test strains on PE catheters was determined in vitro. In these in vitro studies the bacteria and catheters were pretreated with either fibrinogen, fibronectin, albumin or citrated whole plasma or the liquid adherence medium was supplemented with these proteins or the adherence assay was done in citrated whole plasma. The presence of proteins in the adherence medium or pretreatment of the catheter or bacteria with either fibronectin, albumin or whole citrated plasma markedly inhibited bacterial adherence. In contrast, pretreatment of bacteria or both bacteria and catheters with fibrinogen enhanced bacterial adherence suggesting the presence of ligands for fibrinogen on the cell surface of CN Staph.

Biofilm growth of individual and dual strains of Klebsiella oxytoca from the dairy industry on ultrafiltration membranes

“…This protein blocking of attachment has been seen by others [2]. It has also been suggested that fresh proteins present in the liquid inhibit bacterial adhesion [3,8]. It is clear that the behaviour of bioWlms on new and used membranes merits further investigation.…”

“…This involved completing 3 3 = 27 experiments plus some extra experiments as controls. The results were based on four randomly sampled coupons in each experiment.…”

Pathophysiology and Pathogenesis of Osteomyelitis

“…aureus is a common cause of metal-biomaterial, bone-joint, and soft-tissue infections (Petty et al, 1985), while S. epidermidis is more common with polymer-associated implant infections (von Eiff et al, 2002). It has been shown that both fibrinogen (Brokke et al, 1991) and fibronectin (Fischer et al, 1996) deposited in vivo onto the implant surface mediate bacterial adherence. Bacteria compete with host cells for attachment to the implant surface, a phenomenon that has been referred to as 'the race for the surface' (Gristina, 1987) (Fig.…”

Preventing Biofilm Formation on Biomedical Surfaces

“…However, in general, albumin proteins are reported to inhibit bacterial adhesion on material surfaces irrespectively of the type of material [25][26][27], whereas fibrinogen promotes the bacterial adhesion process [26,28]. Fibronectin, on the other hand, shows inhibiting as well as promoting behavior toward bacterial adhesion, depending upon the bacterial strains [28][29][30].…”