Adherence of environmental Arcobacter butzleri and Vibrio spp. isolates to epithelial cells in vitro

Carbone, M.; Maugeri, Teresa L.; Giannone, M.; Gugliandolo, Concetta; Midiri, Angelina; Fera, M. T.

doi:10.1016/s0740-0020(02)00172-7

Cited by 28 publications

(21 citation statements)

References 28 publications

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“…The observation possibly indicates evidence of adherence factors and colonization of the intestine by pilation process described as ability of bacteria to adhere to entero-receptor sites on specific cells surface thereby enabling the organism to colonize the intestine (Carbone et al, 2003).…”

Section: Discussionmentioning

confidence: 84%

Histopathological Changes Associated with Experimental Infection of Arcobacter butzleri in Albino Rats

Adesiji¹,

Benjamin²,

Olaitan³

2010

Sierra Leone j. biomed. res.

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Arcobacters are emerging food borne pathogens potentially associated with prolonged diarrhoea and occasional systemic infections but the pathogenic mechanisms of these bacteria are largely unknown. This study was designed to investigate the pathogenicity of Arcobacter isolates. Two strains of A. butzleri isolated from stool of healthy chickens were confirmed with real time PCR and tested on albino rat by giving a single oral challenge of 10 9 cfu/ml to 65 healthy adult male rats. Five (5) uninfected animals were used as control. Diarrheoal illness occurred in all rats from the fifth day and resolved from day 21 post infection, severe histopathological lesion such as hepatic necrosis, villous erosion, desquamation, matting and necrosis of the segments of small intestine was also observed. In this study, the toxic ileitis necrosis pattern of pathology in the gut of experimentally infected rats could be an indication of observed persistent watery diarrhea associated with the clinical presentation of Arcobacter infection in humans. The pathology of A. butzleri in albino rats had not been previously described, and it appears that the present study is the first report in Nigeria. It may therefore be useful for further investigation.

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Section: Discussionmentioning

confidence: 84%

Histopathological Changes Associated with Experimental Infection of Arcobacter butzleri in Albino Rats

Adesiji¹,

Benjamin²,

Olaitan³

2010

Sierra Leone j. biomed. res.

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“…Mostly abundant in corals, oysters, turbot larval gut, Haemolytic, septicaemic, necrotic Sugumar et al (1998), Thompson et al (2003), Koren and Rosenberg (2006) Arcobacter butzleri Brackish marine environment Human and animal pathogen, secretion of toxic factors Carbone et al (2003), Gugliandolo et al (2007) Pseudoalteromonas group #1 and Vibrio group #4 contain closely related pathogenic and non-pathogenic species structure and activities at cellular level are well documented (Thuesen and Kogure 1989;Do et al 1990;Ritchie et al 2000). TTX-carrying animals are known from different marine, limnic and terrestrial habitats of warmer regions.…”

Section: Discussionmentioning

confidence: 99%

“…Jellyfish Cyanea lamarckii provides the natural habitat for Moritella viscosa, which causes necrotic ''winter ulcer'' in salmons (Lunder et al 2000;Heidarsdottir et al 2008), additionally Arcobacter butzleri, a human pathogen species (Carbone et al 2003;Gugliandolo et al 2007), and finally members of group #4 (see the previous paragraph). The Lions mane jellyfish C. capillata carries the haemolytic species Shewanella waksmanii, a member of group #3 (Ivanova et al 2003), and pathogenic endobionts of the already described Photobacterium profundum and the putatively pathogenic species of groups #1 and #4 (see the previous paragraph).…”

Section: Pathogenic Activities Of Bacterial Endobiontsmentioning

confidence: 99%

Endobiotic bacteria and their pathogenic potential in cnidarian tentacles

Schuett

Doepke

2009

Helgol Mar Res

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Endobiotic bacteria colonize the tentacles of cnidaria. This paper provides first insight into the bacterial spectrum and its potential of pathogenic activities inside four cnidarian species. Sample material originating from Scottish waters comprises the jellyfish species Cyanea capillata and C. lamarckii, hydrozoa Tubularia indivisa and sea anemone Sagartia elegans. Mixed cultures of endobiotic bacteria, pure cultures selected on basis of haemolysis, but also lyophilized samples were prepared from tentacles and used for DGGE-profiling with subsequent phylogenetic analysis of 16S rDNA fragments. Bacteria were detected in each of the cnidarian species tested. Twenty-one bacterial species including four groups of closely related organisms were found in culture material. The species within these groups could not be differentiated from each other (one group of Pseudoalteromonas spp., two groups of Shewanella spp., one group of Vibrio spp.). Each of the hosts exhibits a specific endobacterial spectrum. Solely Cyanea lamarckii harboured Moritella viscosa. Only in Cyanea capillata, members of the Shewanella group #2 and the species Pseudoalteromonas arctica, Shewanella violacea, Sulfitobacter pontiacus and Arcobacter butzleri were detected. Hydrozoa Tubularia indivisa provided an amazingly wide spectrum of nine bacterial species. Exclusively, in the sea anemone Sagartia elegans, the bacterial species P. aliena was found. Overall eleven bacterial species detected were described recently as novel species. Four 16S rDNA fragments generated from lyophilized material displayed extremely low relationship to their next neighbours. These organisms are regarded as members of the endobiotic ''terra incognita''. Since the origin of cnidarian toxins is unclear, the possible pathogenic activity of endobiotic bacteria has to be taken into account. Literature data show that their next neighbours display an interesting diversity of haemolytic, septicaemic and necrotic actions including the production of cytotoxins, tetrodotoxin and R-toxin. Findings of haemolysis tests support the literature data. The potential producers are Endozoicimonas elysicola, Moritella viscosa, Photobacterium profundum, P. aliena, P. tetraodonis, Shewanella waksmanii, Vibrio splendidus, V. aestuarius, Arcobacter butzleri.

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“…Collectively, in those studies 56% (55/59), 20% (9/44), and 85% (164/194) of the strains tested showed adhesion, invasion, and cytotoxicity, respectively (Table 3), with toxicity and adherence therefore being the most commonly observed effects (17,60,66,76,87,114,153,158). The differences observed among the different studies may be due to the origin of the strains (environmental versus clinical) as well as to different cell lines used in those studies (162).…”

Section: Virulence Factorsmentioning

confidence: 94%

“…The pathogenicity and virulence mechanisms of Arcobacter species are still poorly understood, despite several studies having investigated their adhesion capacity (17,60,66,76,114,153), invasiveness (47,66,114,153), and cytotoxicity (17,60,87,114,153,158) in several cell lines (Table 3). Collectively, in those studies 56% (55/59), 20% (9/44), and 85% (164/194) of the strains tested showed adhesion, invasion, and cytotoxicity, respectively (Table 3), with toxicity and adherence therefore being the most commonly observed effects (17,60,66,76,87,114,153,158).…”

Section: Virulence Factorsmentioning

confidence: 99%

Taxonomy, Epidemiology, and Clinical Relevance of the Genus Arcobacter

2011

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SUMMARY The genus Arcobacter , defined almost 20 years ago from members of the genus Campylobacter , has become increasingly important because its members are being considered emergent enteropathogens and/or potential zoonotic agents. Over recent years information that is relevant for microbiologists, especially those working in the medical and veterinary fields and in the food safety sector, has accumulated. Recently, the genus has been enlarged with several new species. The complete genomes of Arcobacter butzleri and Arcobacter nitrofigilis are available, with the former revealing diverse pathways characteristic of free-living microbes and virulence genes homologous to those of Campylobacter. The first multilocus sequence typing analysis showed a great diversity of sequence types, with no association with specific hosts or geographical regions. Advances in detection and identification techniques, mostly based on molecular methods, have been made. These microbes have been associated with water outbreaks and with indicators of fecal pollution, with food products and water as the suspected routes of transmission. This review updates this knowledge and provides the most recent data on the taxonomy, species diversity, methods of detection, and identification of these microbes as well as on their virulence potential and implication in human and animal diseases.

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Adherence of environmental Arcobacter butzleri and Vibrio spp. isolates to epithelial cells in vitro

Cited by 28 publications

References 28 publications

Histopathological Changes Associated with Experimental Infection of Arcobacter butzleri in Albino Rats

Histopathological Changes Associated with Experimental Infection of Arcobacter butzleri in Albino Rats

Endobiotic bacteria and their pathogenic potential in cnidarian tentacles

Taxonomy, Epidemiology, and Clinical Relevance of the Genus Arcobacter

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