Adherence of human mesenchymal stem cells on Ti and TiO2 nano-columnar surfaces fabricated by glancing angle sputter deposition

Motemani, Yahya; Greulich, C.; Khare, Chinmay; Lopian, Michael; Buenconsejo, Pio John S.; Schildhauer, Thomas; Ludwig, Alfred; Köller, Manfred

doi:10.1016/j.apsusc.2013.12.022

Cited by 29 publications

(20 citation statements)

References 41 publications

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“…[3][4][5][6] Characterized by a high self-renewal rate, these cells are regarded as a potential candidate for bone tissue engineering, 3,7 as well as for use within in vitro models for tissue-biomaterial response testing. 8,9 Recently, human adipose-derived stem cells (ADSCs) have aroused tremendous research interest as alternative sources of MSCs primarily because of their ease of isolation, extensive proliferation ability, and hypoimmunogenic nature. 10 Unlike MSCs, they represent an abundant source of pluripotent stem cells that can be easily isolated from subcutaneous adipose tissue through minimally invasive procedures such as Taken together with all these advantages, the use of ADSCs as an alternative stem cell source for various biomedical applications holds great promise.…”

Section: Introductionmentioning

confidence: 99%

Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces

Tan¹,

Tay²,

Chua³

et al. 2014

IJN

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Two important criteria of an ideal biomaterial in the field of stem cells research are to regulate the cell proliferation without the loss of its pluripotency and to direct the differentiation into a specific cell lineage when desired. The present study describes the influence of TiO 2 nanofibrous surface structures on the regulation of proliferation and stemness preservation of adipose-derived stem cells (ADSCs). TiO 2 nanofiber arrays were produced in situ onto Ti-6Al-4V substrate via a thermal oxidation process and the successful fabrication of these nanostructures was confirmed by field emission scanning electron microscopy (FESEM), energy dispersive spectrometer (EDS), X-ray diffractometer (XRD), and contact angle measurement. ADSCs were seeded on two types of Ti-6Al-4V surfaces (TiO 2 nanofibers and flat control), and their morphology, proliferation, and stemness expression were analyzed using FESEM, AlamarBlue assay, flow cytometry, and quantitative real-time polymerase chain reaction (qRT-PCR) after 2 weeks of incubation, respectively. The results show that ADSCs exhibit better adhesion and significantly enhanced proliferation on the TiO 2 nanofibrous surfaces compared to the flat control surfaces. The greater proliferation ability of TiO 2 nanofibrous surfaces was further confirmed by the results of cell cycle assay. More importantly, TiO 2 nanofibrous surfaces significantly upregulate the expressions of stemness markers Sox-2, Nanog3, Rex-1, and Nestin. These results demonstrate that TiO 2 nanofibrous surfaces can be used to enhance cell adhesion and proliferation while simultaneously maintaining the stemness of ADSCs, thereby representing a promising approach for their potential application in the field of bone tissue engineering as well as regenerative therapies.

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Section: Introductionmentioning

confidence: 99%

Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces

Tan¹,

Tay²,

Chua³

et al. 2014

IJN

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“…Several attempts were done in the literature in order to increase the TiO 2 layer porosity above the Thornton limit by using sputtering or evaporation approaches which generated cauliflower37, penniform38 or zig-zag39 structures. Some other attempts were done by GLancing Angle Deposition (GLAD)40 to deposit Ti nanostructures414243 which requires ex-situ oxidation for TiO 2 reaction; or by using array of template materials (e.g. polystyrene nano-spheres) to exploit their shadowing effect during TiO 2 growth44.…”

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confidence: 99%

Multi-Scale-Porosity TiO2 scaffolds grown by innovative sputtering methods for high throughput hybrid photovoltaics

Sanzaro

Smecca

Mannino

et al. 2016

Sci Rep

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We propose an up-scalable, reliable, contamination-free, rod-like TiO2 material grown by a new method based on sputtering deposition concepts which offers a multi-scale porosity, namely: an intra-rods nano-porosity (1–5 nm) arising from the Thornton’s conditions and an extra-rods meso-porosity (10–50 nm) originating from the spatial separation of the Titanium and Oxygen sources combined with a grazing Ti flux. The procedure is simple, since it does not require any template layer to trigger the nano-structuring, and versatile, since porosity and layer thickness can be easily tuned; it is empowered by the lack of contaminations/solvents and by the structural stability of the material (at least) up to 500 °C. Our material gains porosity, stability and infiltration capability superior if compared to conventionally sputtered TiO2 layers. Its competition level with chemically synthesized reference counterparts is doubly demonstrated: in Dye Sensitized Solar Cells, by the infiltration and chemisorption of N-719 dye (∼1 × 1020 molecules/cm3); and in Perovskite Solar Cells, by the capillary infiltration of solution processed CH3NH3PbI3 which allowed reaching efficiency of 11.7%. Based on the demonstrated attitude of the material to be functionalized, its surface activity could be differently tailored on other molecules or gas species or liquids to enlarge the range of application in different fields.

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“…The deposition method glancing angle deposition (GLAD) enables the creation of nanostructured thin films. This method is mostly implemented in physical vapor deposition (PVD) techniques [2], [3]. However, there are also other techniques that use this method, e.g., electron beam evaporation [4].…”

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confidence: 99%

“…The GLAD method is based on the variation of the particle's incidence angle toward the substrate. The variation of the incidence angle provides the formation of thin films with oriented columnar nanostructure [5], which subsequently influences a wide range of the thin films properties [2]- [4] (e.g., specific surface, roughness, photocatalytic activity in case of TiO 2 thin films [6]) and thus their applications. Nanostructured TiO 2 thin films were deposited by GLAD PVD-reactive magnetron sputtering (Fig.…”

mentioning

confidence: 99%

Sputter Deposition of Nanostructured TiO₂ Thin Films

Horáková

Cerny

Kriz

et al. 2014

IEEE Trans. Plasma Sci.

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A physical vapor deposition (PVD) method using plasma discharge-glancing angle deposition (GLAD), based on a variation of the particle's incidence angle to the substrate, enables the formation of TiO 2 thin films with oriented columnar nanostructure. The incidence angle influences crystalline, nanostructure, and surface properties of the deposited films. Images illustrating the GLAD PVD method and scanning electron microscopy images of the TiO 2 thin films deposited with different incidence angles α are presented.

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Adherence of human mesenchymal stem cells on Ti and TiO2 nano-columnar surfaces fabricated by glancing angle sputter deposition

Cited by 29 publications

References 41 publications

Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces

Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces

Multi-Scale-Porosity TiO2 scaffolds grown by innovative sputtering methods for high throughput hybrid photovoltaics

Sputter Deposition of Nanostructured TiO₂ Thin Films

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Adherence of human mesenchymal stem cells on Ti and TiO2 nano-columnar surfaces fabricated by glancing angle sputter deposition

Cited by 29 publications

References 41 publications

Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in&nbsp;situ TiO2 nanofibrous surfaces

Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in&nbsp;situ TiO2 nanofibrous surfaces

Multi-Scale-Porosity TiO2 scaffolds grown by innovative sputtering methods for high throughput hybrid photovoltaics

Sputter Deposition of Nanostructured TiO2 Thin Films

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Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces

Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces

Sputter Deposition of Nanostructured TiO₂ Thin Films