Adherence of Staphylococcus epidermidis to fibrin-platelet clots in vitro mediated by lipoteichoic acid

Chugh, T.D.; Burns, Gary R.; H, Shuhaiber; Bahr, George M.

doi:10.1128/iai.58.2.315-319.1990

Cited by 52 publications

(11 citation statements)

References 22 publications

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“…When platelet tethering is initiated the bacteria and platelets may become colocalized on a surface even without interacting directly with each other (57). It is noteworthy that adherence of streptococci and staphylococci to an immobilized fibrin matrix is largely increased by platelets (59,60). However, soluble fibrin and the release of ␣-granule proteins, particularly TSP1, are required for the direct association of staphylococci with platelets (61).…”

Section: Discussionmentioning

confidence: 99%

Thrombospondin‐1 promotes cellular adherence of Gram‐positive pathogensviarecognition of peptidoglycan

Rennemeier¹,

Hammerschmidt²,

Niemann³

et al. 2007

FASEB j.

107

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Thrombospondin-1 (TSP1) is a matricellular glycoprotein that has key roles in interactions between human cells and components of the extracellular matrix. Here we report a novel role for the lectin TSP1 in pathogen-host interactions. Binding assays and flow cytometric analysis demonstrate that Streptococcus pneumoniae and other gram-positive pathogens including S. pyogenes, Staphylococcus aureus, and Listeria monocytogenes interact specifically with human TSP1. We also show for the first time that host cell-bound TSP1 promotes adherence of gram-positive pathogens to human epithelial and endothelial cell lines. Pretreatment of bacteria with sodium periodate but not Pronase E substantially reduced TSP1-mediated bacterial adherence to host cells, suggesting that a glycoconjugate on the bacterial cell surface functions as the receptor for TSP1. Lipoteichoic acids did not affect TSP1-mediated adherence of S. pneumoniae to host cells. In contrast, attachment of S. pneumoniae and other gram-positive pathogens to host cells via TSP1 was blocked by soluble peptidoglycan, indicating recognition of bacterial peptidoglycan by TSP1. In conclusion, our results demonstrate that recognition of gram-positive pathogens by TSP1 promotes bacterial colonization of host tissue cells. In this scenario, peptidoglycan functions as adhesin and TSP1 acts as a molecular bridge linking gram-positive bacteria with receptors on the host cell.

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Section: Discussionmentioning

confidence: 99%

Thrombospondin‐1 promotes cellular adherence of Gram‐positive pathogensviarecognition of peptidoglycan

Rennemeier¹,

Hammerschmidt²,

Niemann³

et al. 2007

FASEB j.

107

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show abstract

“…Future strategies designed to reduce or eliminate implant-associated infections will likely target the docking stage of bacterial adhesion to subvert colonization. Primary adhesion to plastic has been demonstrated in vitro for both slime-positive and -negative strains of S. epidermidis (5,10) and, therefore, is independent of slime production. It is probably simplistic to think that primary bacterial adhesion in vivo involves only hydrophobic or ligand-specific interactions.…”

mentioning

confidence: 98%

The Effects of Magnesium, Calcium, EDTA, and pH on the In Vitro Adhesion of Staphylococcus epidermidis to Plastic

Dunne

Burd

1992

Microbiology and Immunology

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“…Host proteins do not enhance adherence of coagulase-negative staphylococci to biomaterials.Coagulase-negative staphylococci (CoNS) have become major nosocomial pathogens because of their capacity to colonize plastic biomaterials used in catheters and prosthetic devices (7). Several mechanisms have been proposed to explain this adherence to biomaterials, but the problem is far from solved (6,10,18,20). Recent studies suggest that host proteins coated onto biomaterials promote adherence of CoNS (10, 21).We have proposed that the capsular polysaccharide/adhesin (PS/A) mediates the initial adherence of CoNS to plastic biomaterials.…”

mentioning

confidence: 99%

Blood proteins do not promote adherence of coagulase-negative staphylococci to biomaterials

et al. 1991

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We studied the effects of in vitro and in vivo coating of catheters with human blood proteins on binding of coagulase-negative staphylococci. Coating resulted in no enhancement of binding. Catheters coated in vitro bound fewer organisms than uncoated catheters. Host proteins do not enhance adherence of coagulase-negative staphylococci to biomaterials.Coagulase-negative staphylococci (CoNS) have become major nosocomial pathogens because of their capacity to colonize plastic biomaterials used in catheters and prosthetic devices (7). Several mechanisms have been proposed to explain this adherence to biomaterials, but the problem is far from solved (6,10,18,20). Recent studies suggest that host proteins coated onto biomaterials promote adherence of CoNS (10, 21).We have proposed that the capsular polysaccharide/adhesin (PS/A) mediates the initial adherence of CoNS to plastic biomaterials. We noted that strains of CoNS which produced PS/A and slime (PS/A' slime') (20) adhered in high numbers to native, uncoated plastics, such as silicon elastomer. PS/Aslime-strains adhere to plastics in much smaller numbers. In the present study, we examined the effect of precoating catheters in vitro with human blood, serum, and purified blood proteins on adherence of both PS/A' slime' and PS/Aslimestrains of CoNS. We also studied adherence of CoNS to catheters coated in vivo with blood proteins after insertion into volunteers or hospitalized patients.CoNS were isolated from patients with clear-cut clinical diagnoses of catheter-related sepsis, dialysis-related peritonitis, endocarditis, or cerebrospinal fluid shunt infection and were identified as Staphylococcus epidermidis by a Sceptor System (Staphylococcus MIC/ID Panel; Becton Dickinson, Towson, Md.). Strains were categorized as PS/A' or PS/Aby enzyme-linked immunosorbent assay inhibition using polyclonal antibody specific for the PS/A antigen purified from S. epidermidis RP62A (ATCC 35984) (20). PS/A' strains showed a mean (+ standard deviation) of 75% + 12% inhibition of binding of PS/A-specific polyclonal rabbit antibody, while PS/Astrains showed a mean of 3.1% + 7% inhibition. Slime production was quantitated by measuring the optical density at 490 nm of the safranin-stained biofilms produced by inoculation of 105 to 106 CFU of CoNS per ml followed by overnight growth at 37°C in tryptic soy broth (TSB) in flat-bottomed 96-well tissue culture plates (Costar, Cambridge, Mass.). PS/A' strains produced a mean optical density of 1.25 ± 0.4, while PS/Astrains produced a mean optical density of 0.34 ± 0.08. Staphylococcus aureus Reynolds, Becker, Wood 46, and Cowan 1 were provided by Jean Lee of Harvard Medical School and were also used in the studies. * Corresponding author.To measure adherence to catheter tubing, CoNS were grown at 37°C overnight in static TSB cultures containing 25 ,uCi of Na [1-'4C]acetate (Du Pont, NEN Research Products, Boston, Mass.). The bacteria were sedimented by centrifugation and suspended in TSB. On the basis of previous reports (10, 21) that a protein ...

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Adherence of Staphylococcus epidermidis to fibrin-platelet clots in vitro mediated by lipoteichoic acid

Cited by 52 publications

References 22 publications

Thrombospondin‐1 promotes cellular adherence of Gram‐positive pathogensviarecognition of peptidoglycan

Thrombospondin‐1 promotes cellular adherence of Gram‐positive pathogensviarecognition of peptidoglycan

The Effects of Magnesium, Calcium, EDTA, and pH on the In Vitro Adhesion of Staphylococcus epidermidis to Plastic

Blood proteins do not promote adherence of coagulase-negative staphylococci to biomaterials

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