Adherence properties ofPseudomonaspili to epithelial cells of the human cornea

Rw, Reichert; Nd, Das; Zs, Zam

doi:10.3109/02713688209000772

Cited by 28 publications

(7 citation statements)

References 13 publications

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“…Thus, this bacterial strain may be poorly or not pil- iated. P. fluorescens previously shown to adhere to plant roots, meat and human corneal epithelial cells [9][10][11] adhered to A549 cells in a dose-dependent manner. This adherence to human pulmonary epithelial cells is consistent with the P. fluorescens implication in nosocomial respiratory infections [7,8].…”

Section: Discussionmentioning

confidence: 99%

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Effects of Antibiotics on Adherence of Pseudomonas aeruginosa and Pseudomonas fluorescens to A549 Pneumocyte Cells

Martino

Rebière-Huët²,

Hulen³

2000

Chemotherapy

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The aim of this study was to evaluate the effect of antibiotics at subminimal inhibitory concentrations (sub-MIC) on fluorescent pseudomonas adherence to A549 pneumocyte cells. Pseudomonas fluorescens MF0 isolated from contaminated raw milk and Pseudomonas aeruginosa NK125502 isolated from a cystic fibrosis patient’s lung adhered to A549 cells. As previously shown for P. aeruginosa, P. fluorescens bound to A549 cells in a dose-dependent manner over a wide range of bacterial concentrations. Bacterial growth in the presence of polymyxin B or gentamicin at MIC/2 had no effect on the adherence of NK125502 and MF0 to A549 cells. Instead, MIC/2 and MIC/8 of cefsulodin or chloramphenicol decreased the adherence of the two strains. A decrease in MF0 adherence was also observed with cefsulodin at MIC/32. We conclude that, in addition to their antibacterial activity, cefsulodin and chloramphenicol could be effective in preventing Pseudomonas adherence to respiratory epithelium.

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Section: Discussionmentioning

confidence: 99%

“…Since this microorganism is a typical rhizosphere leaving bacterium and a usual food-contaminating species, some studies have been conducted to investigate P. fluorescens adherence to vegetables and meat [9,10], but little is known about adherence of this microorganism to human tissues [11].…”

Section: Introductionmentioning

confidence: 99%

Effects of Antibiotics on Adherence of Pseudomonas aeruginosa and Pseudomonas fluorescens to A549 Pneumocyte Cells

Martino

Rebière-Huët²,

Hulen³

2000

Chemotherapy

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“…It has been suggested that the adherence of P. aeruginosa to eukaryotic cells, such as buccal, corneal, tracheal and phagocytic cells, is mediated by fimbriae (Woods et al, 19806;Reichert et al, 1983;Paranchych et al, 1986). In our study, in as much as all the bacterial strains used were fimbriate, we could not attribute the observed difference to the capability of fimbriate bacteria to adhere to HLE-treated mucosa.…”

Section: Discussionmentioning

confidence: 99%

Adherence of Pseudomonas aeruginosa to respiratory epithelium and the effect of leucocyte elastase

Plotkowski

Beck²,

Tournier³

et al. 1989

Journal of Medical Microbiology

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Summary. The tracheobronchial secretions from patients with cystic fibrosis often contain high amounts of free proteases. To evaluate whether human leucocyte elastase (HLE) can favour the persistence of bacterial airways infection, we exposed the frog palate mucosa to HLE and then to radiolabelled Pseudomonas aeruginosa and followed the sequence of events by scanning electronmicroscopy. In response to HLE there was a marked outpouring of mucus and a desquamation of the epithelium. P. aeruginosa was shown to adhere to recently secreted granules of mucus and to the exposed submucosal underlying connective tissues. For the eight different bacterial strains studied, a significative adherence to HLE-injured mucosa was observed only in strains that possessed internal haemagglutinating activity. Neither the presence of fimbriae, nor of the mucoid exopolysaccharide, nor of the bacterial surface haemagglutinating activity could be related to adherence of P. aeruginosa to the injured mucosa. These results support the hypothesis that HLE enhances bacterial infection of the respiratory mucosa both by inducing mucus hypersecretion and by exposing receptors to the microbial adhesins. It is also suggested that P. aeruginosa internal lectins may be implicated in adherence to host tissues.

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“…Attachment of bacteria to host tissues involves interactions between molecules present on both the microorganism itself and on the host cell surface, and determines the tissue tropism of the subsequent infection. Typical bacterial factors involved in this step are the pili, flagella, exopolysacharides, lipopolysaccharide (LPS) and outer membrane proteins [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

In vitro identification of two adherence factors required for in vivo virulence of Pseudomonas fluorescens

Pimenta

Martino

Bouder

et al. 2003

Microbes and Infection

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Adherence properties ofPseudomonaspili to epithelial cells of the human cornea

Cited by 28 publications

References 13 publications

Effects of Antibiotics on Adherence of <i>Pseudomonas aeruginosa</i> and <i>Pseudomonas fluorescens</i> to A549 Pneumocyte Cells

Effects of Antibiotics on Adherence of <i>Pseudomonas aeruginosa</i> and <i>Pseudomonas fluorescens</i> to A549 Pneumocyte Cells

Adherence of Pseudomonas aeruginosa to respiratory epithelium and the effect of leucocyte elastase

In vitro identification of two adherence factors required for in vivo virulence of Pseudomonas fluorescens

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