Dear Sir,It is well known that women who are infected with the human immunodeficiency virus (HIV) have higher rates of infection with human Papillomaviruses (HPV) 1,2 and that these women present an approximately 10-fold higher risk for invasive cervical cancer (ICC) than age-matched women in the general population. 3 In addition to the high frequency of HPV infection, the increased ICC risk in HIVpositive women could be explained by the immunosuppressive effect of HIV, which may accelerate HPV-related oncogenesis. 4,5 Moreover, because of life-styles often associated with HIV infection (e.g., use of intravenous drugs and prostitution), HIV-positive women may be less likely to receive appropriate cervical screening through Papanicolaou (Pap)-smear than HIV-negative women.In this epidemiological research, we investigated I) the extent to which HIV-infected women were aware that Pap-smear could prevent cervical cancer, ii) factors eventually associated with lack of awareness of Pap-smear, and iii) whether lack of awareness affected the use of Pap-smear screening.A cross-sectional study was conducted between January 2003 and June 2004 at the HIV Outpatient Gynaecological Clinic of the National Institute for Infectious Disease L. Spallanzani, Rome. During this period, 534 HIV-positive women were referred by infectious disease specialists for gynaecological examination, including Pap-smear and, when appropriate, colposcopy. Of these 534 HIV-positive women, 147 were not timely traced. According to a protocol approved by the Ethical Committee of the National Institute for Infectious Disease L. Spallanzani, Rome, 387 women (72.5%) were thus invited to take part in the study. Three hundred eighty-one of them accepted participation and signed the informed consent. Following the aim of this analysis, 44 out of the 381 HIV-positive women who accepted participation were excluded because of missing information on the study endpoint: the remaining 337 HIV-positive women constitute the study group.Relevant information was collected through a questionnaire-based interview that was conducted, before the gynaecological examination, by 2 gynaecologists (CP and GP). To assess awareness of the Pap-smear, study participants were first asked if they were ever told about Pap-smear and, second, if they knew that Pap-smear was a cancer preventive tool. Following the aims of this analysis, women who knew that a Pap-test was used to prevent cervical cancer (or cancer of the uterus) were considered aware of Pap-smear. Other collected data included sociodemographic factors (e.g., age, education and country of origin), smoking and reproductive history, sexual habits (e.g., age at first intercourse, number of partners and contraceptive methods) and history of HIV infection (e.g., date of first seropositive test and mode of HIV acquisition). History of Papsmear was investigated in detail to assess lifetime testing, age at first smear, number of smears done in the 5 years and in the last year preceding the interview, and a self-reported history o...