Adhesin-Encoding Genes from Shiga Toxin-Producing Escherichia coli Are More Prevalent in Atypical than in Typical Enteropathogenic E. coli

Gomes, Tânia A. T.; Hernandes, Rodrigo T.; Torres, Alfredo G.; Salvador, Fábia A.; Guth, Beatriz E. C.; Vaz, Tânia; Irino, Kinue; Silva, Rosa Maria; Vieira, Mônica A. M.

doi:10.1128/jcm.00779-11

Cited by 26 publications

(22 citation statements)

References 31 publications

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“…Almost all EPEC isolates (32 aEPEC and 1 tEPEC) were FAS positive (data not shown), one aEPEC isolate being the only exception (C144‐1) interacted with HeLa cells in an undefined pattern. LAL has been the most common adherence pattern detected among aEPEC , and the association of this feature with the ability to produce AE lesion has been found in association with diarrheal disease .…”

Section: Resultsmentioning

confidence: 97%

Diarrheagenic Escherichia coli pathotypes investigation revealed atypical enteropathogenic E. coli as putative emerging diarrheal agents in children living in Botucatu, São Paulo State, Brazil

Dias

Santos

et al. 2016

APMIS

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The aim of the present study was to investigate the prevalence of Diarrheagenic Escherichia coli (DEC) pathotypes, a leading cause of diarrhea worldwide, among diarrheal and healthy children, up to 5 years of age, living in the city of Botucatu, São Paulo, Brazil. DEC, investigated by PCR detection of virulence factor-encoding genes associated with the distinct pathotypes, was isolated from 18.0% of the patients, and 19.0% of the controls, with enteroaggregative E. coli (EAEC), the most frequent pathotype, being detected in equal proportion between patients and controls (10.0%). Among the enteropathogenic E. coli (EPEC) isolates, only one isolate was able to produce the localized adherence pattern to HeLa cells, being thus the only typical EPEC identified. All the remaining EPEC were classified as atypical (aEPEC), and detected in 8.0% and 8.5% of the patients and controls, respectively. Regarding the serotypes, 26.5% of the analyzed EPEC isolates belonged to classical EPEC-serogroups, and the only two STEC found were serotyped as O26:H11 (patient) and O119:H7 (control). Antimicrobial susceptibility tests revealed that 43.6%, 29.5% and 2.6% of the DEC isolates were resistant to ampicillin, cotrimoxazole and gentamicin, respectively. Our data indicate that EAEC remains prevalent among children living in Botucatu, and revealed atypical EPEC as emerging putative diarrheal agents in this geographical region.

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Section: Resultsmentioning

confidence: 97%

Diarrheagenic Escherichia coli pathotypes investigation revealed atypical enteropathogenic E. coli as putative emerging diarrheal agents in children living in Botucatu, São Paulo State, Brazil

Dias

Santos

et al. 2016

APMIS

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“…Intimins classified as beta1, epsilon1 and theta appear as the most frequent among aEPEC 78, 82, 83, 84, 85. In addition, some aEPEC strains bear adhesive-encoding genes that have been originally described in other DEC pathotypes and/or in extraintestinal pathogenic E. coli 79, 80, 82, 86, 87, 88. This observation suggests that aEPEC could employ additional adherence mechanisms besides the Tir-intimin interaction.…”

Section: Typical and Atypical Enteropathogenic E Colimentioning

confidence: 87%

“…Most aEPEC produce adherence patterns categorized as LA-like, with loosened microcolonies compared to those of the tEPEC LA pattern 12, 76, 77. In addition, some isolates express the aggregative (AA) or diffuse (DA) patterns of adherence, which are characteristics of the EAEC and DAEC pathotypes, respectively,20, 78 or adhere in undefined patterns or are non-adherent 20, 78, 79, 80. Remarkably, the epithelial cell adherence phenotype displayed by aEPEC is determined in prolonged assays (6 h) of bacteria-cell interaction 12, 76, 77.…”

Section: Typical and Atypical Enteropathogenic E Colimentioning

confidence: 99%

Diarrheagenic Escherichia coli

Gomes

Elias

Scaletsky

et al. 2016

Brazilian Journal of Microbiology

433

353

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Most Escherichia coli strains live harmlessly in the intestines and rarely cause disease in healthy individuals. Nonetheless, a number of pathogenic strains can cause diarrhea or extraintestinal diseases both in healthy and immunocompromised individuals. Diarrheal illnesses are a severe public health problem and a major cause of morbidity and mortality in infants and young children, especially in developing countries. E. coli strains that cause diarrhea have evolved by acquiring, through horizontal gene transfer, a particular set of characteristics that have successfully persisted in the host. According to the group of virulence determinants acquired, specific combinations were formed determining the currently known E. coli pathotypes, which are collectively known as diarrheagenic E. coli. In this review, we have gathered information on current definitions, serotypes, lineages, virulence mechanisms, epidemiology, and diagnosis of the major diarrheagenic E. coli pathotypes.

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“…Other studies have shown the presence of adherence factors, other than EspA, in aEPEC (21,23,24), but none of them are ATs. Also, genes encoding proteins involved in biofilm formation (such as cah, ehaABCDJ, sab, and tibA) might be important in the persistence of both EPEC groups in the intestine.…”

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confidence: 99%

Autotransporter Protein-Encoding Genes of Diarrheagenic Escherichia coli Are Found in both Typical and Atypical Enteropathogenic E. coli Strains

Abreu

Bueris

Porangaba

et al. 2013

Appl Environ Microbiol

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c Autotransporter (AT) protein-encoding genes of diarrheagenic Escherichia coli (DEC) pathotypes (cah, eatA, ehaABCDJ, espC, espI, espP, pet, pic, sat, and tibA) were detected in typical and atypical enteropathogenic E. coli (EPEC) in frequencies between 0.8% and 39.3%. Although these ATs have been described in particular DEC pathotypes, their presence in EPEC indicates that they should not be considered specific virulence markers.

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Adhesin-Encoding Genes from Shiga Toxin-Producing Escherichia coli Are More Prevalent in Atypical than in Typical Enteropathogenic E. coli

Cited by 26 publications

References 31 publications

Diarrheagenic Escherichia coli pathotypes investigation revealed atypical enteropathogenic E. coli as putative emerging diarrheal agents in children living in Botucatu, São Paulo State, Brazil

Diarrheagenic Escherichia coli pathotypes investigation revealed atypical enteropathogenic E. coli as putative emerging diarrheal agents in children living in Botucatu, São Paulo State, Brazil

Diarrheagenic Escherichia coli

Autotransporter Protein-Encoding Genes of Diarrheagenic Escherichia coli Are Found in both Typical and Atypical Enteropathogenic E. coli Strains

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