Adhesins as Targets for Vaccine Development

Wizemann, Theresa; Adamou, John E.; Langermann, Solomon

doi:10.3201/eid0503.990310

Cited by 155 publications

(113 citation statements)

References 59 publications

Supporting

Mentioning

111

Contrasting

Unclassified

Order By: Relevance

“…Adherence is the first step in colonization of hosts by bacterial pathogens, and bacterial adhesins may be useful vaccine antigens [6]. In S. pyogenes, adherence could be mediated by different surface exposed proteins, which include among others, fibronectin binding proteins (FBPs) [7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Streptococcus pyogenes prtFII, but not sfbI, sfbII or fbp54, is represented more frequently among invasive-disease isolates of tropical Australia

et al. 2002

View full text Add to dashboard Cite

Streptococcus pyogenes (group A streptococcus) strains may express several distinct ®bronectinbinding proteins (FBPs) which are considered as major streptococcal adhesins. Of the FBPs, SfbI was shown in Šitro to promote internalization of the bacterium into host cells and has been implicated in persistence. In the tropical Northern Territory, where group A streptococcal infection is common, multiple genotypes of the organism were found among isolates from invasive disease cases and no dominant strains were observed. To determine whether any FBPs is associated with invasive disease propensity of S. pyogenes, we have screened streptococcal isolates from bacteraemic and necrotizing fasciitis patients and isolates from uncomplicated infections for genetic endowment of 4 FBPs. No difference was observed in the distribution of sfbII, fbp54 and sfbI between the blood isolates and isolates from uncomplicated infection. We conclude that the presence of sfbI does not appear to promote invasive diseases, despite its association with persistence. We also show a higher proportion of group A streptococcus strains isolated from invasive disease cases possess prtFII when compared to strains isolated from non-invasive disease cases. We suggest that S. pyogenes may recruit different FBPs for different purposes. Keywords CMMB SUMMARYStreptococcus pyogenes (group A streptococcus) strains may express several distinct fibronectinbinding proteins (FBPs) which are considered as major streptococcal adhesins. Of the FBPs, SfbI was shown in itro to promote internalization of the bacterium into host cells and has been implicated in persistence. In the tropical Northern Territory, where group A streptococcal infection is common, multiple genotypes of the organism were found among isolates from invasive disease cases and no dominant strains were observed. To determine whether any FBPs is associated with invasive disease propensity of S. pyogenes, we have screened streptococcal isolates from bacteraemic and necrotizing fasciitis patients and isolates from uncomplicated infections for genetic endowment of 4 FBPs. No difference was observed in the distribution of sfbII, fbp54 and sfbI between the blood isolates and isolates from uncomplicated infection. We conclude that the presence of sfbI does not appear to promote invasive diseases, despite its association with persistence. We also show a higher proportion of group A streptococcus strains isolated from invasive disease cases possess prtFII when compared to strains isolated from non-invasive disease cases. We suggest that S. pyogenes may recruit different FBPs for different purposes.

show abstract

Section: Introductionmentioning

confidence: 99%

Streptococcus pyogenes prtFII, but not sfbI, sfbII or fbp54, is represented more frequently among invasive-disease isolates of tropical Australia

et al. 2002

View full text Add to dashboard Cite

show abstract

“…Recent research has greatly expanded the molecular details of the specific adhesin:ligand couples employed by Gram-positive bacteria (S. epidermidis, S. aureus, Group A and B Streptococcus, E. faecalis), Gram-negative bacteria (P. aeruginosa, Klebsiella pneumoniae, P. gingivalis, E. coli), and yeast (Candida spp.) to colonize and infect living tissue and implanted biomedical devices (Wizemann et al, 1999). Thus, the concept of biomaterials designed to engineer infection immunity, targeting specific adhesins, could be applied to numerous situations.…”

Section: Engineering Infection Immunitymentioning

confidence: 99%

“…Both an innate and an induced antibody response could prevent attachment and abrogate colonization. The ideal antigens to promote both levels of immune response would be the very surface proteins (bacterial ''adhesins'') that mediate specific bacterial cell adhesion to ligands present on host tissue or device surfaces (Wizemann et al, 1999). Given that bacterial specific adhesion can trigger expression of many virulence factors leading to acute and chronic inflammation, a vaccine approach that blocks bacterial adhesion may have multiple advantages.…”

Section: Engineering Infection Immunitymentioning

confidence: 99%

Medical biofilms

Bryers

2008

Biotech & Bioengineering

665

527

View full text Add to dashboard Cite

For more than two decades, Biotechnology and Bioengineering has documented research focused on natural and engineered microbial biofilms within aquatic and subterranean ecosystems, wastewater and waste-gas treatment systems, marine vessels and structures, and industrial bioprocesses. Compared to suspended culture systems, intentionally engineered biofilms are heterogeneous reaction systems that can increase reactor productivity, system stability, and provide inherent cell:product separation. Unwanted biofilms can create enormous increases in fluid frictional resistances, unacceptable reductions in heat transfer efficiency, product contamination, enhanced material deterioration, and accelerated corrosion. Missing from B&B has been an equivalent research dialogue regarding the basic molecular microbiology, immunology, and biotechnological aspects of medical biofilms. Presented here are the current problems related to medical biofilms; current concepts of biofilm formation, persistence, and interactions with the host immune system; and emerging technologies for controlling medical biofilms.

show abstract

“…Furthermore, E. coli expressing both P and type 1 fimbriae were found to activate TLR4 in mucosal tissues (6). FimH, the adhesin portion of type 1 fimbriae, is involved in bacterial attachment to epithelial cells through interactions with mannose (5,7). Although the role of FimH in pathogenicity is well appreciated, there is no report of FimH inducing innate host responses.…”

mentioning

confidence: 99%

Cutting Edge: FimH Adhesin of Type 1 Fimbriae Is a Novel TLR4 Ligand

Mossman

Mian

Lauzon

et al. 2008

The Journal of Immunology

112

View full text Add to dashboard Cite

Several TLR ligands of bacterial origin induce innate immune responses. Although FimH, the adhesin portion of type 1 fimbria, plays an important role in the pathogenicity of some Gram-negative bacteria, its ability to stimulate the innate immune system via TLR signaling remains unclear. In this study we report that FimH induces potent innate responses in a MyD88-dependent fashion. The FimH-induced innate activity was restricted to cells expressing TLR4. In addition, FimH was able to bind directly to TLR4. More importantly, cells unresponsive to LPS were responsive to FimH and the presence or absence of MD-2 and CD14 had no effect on FimH activity. Our data suggest that TLR4 is a functional receptor for the adhesin portion of bacterial type 1 fimbria.

show abstract

Adhesins as Targets for Vaccine Development

Cited by 155 publications

References 59 publications

Streptococcus pyogenes prtFII, but not sfbI, sfbII or fbp54, is represented more frequently among invasive-disease isolates of tropical Australia

Streptococcus pyogenes prtFII, but not sfbI, sfbII or fbp54, is represented more frequently among invasive-disease isolates of tropical Australia

Medical biofilms

Cutting Edge: FimH Adhesin of Type 1 Fimbriae Is a Novel TLR4 Ligand

Contact Info

Product

Resources

About