Adhesion among neural cells of the chick embryo. II. Purification and characterization of a cell adhesion molecule from neural retina.

Thiéry, Jean‐Paul; Brackenbury, Robert; Rutishauser, Urs; Edelman, Gerald M.

doi:10.1016/s0021-9258(17)39926-x

Cited by 405 publications

(52 citation statements)

References 10 publications

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“…Within a few years, his group and others identified the founding members of the calcium-dependent cadherin superfamily, cdh1-3 (Takeichi, 2018). In parallel, Edelman and colleagues (Thiery et al, 1977) described the first calcium-independent adhesion molecule, N-CAM, now known to be a member of the immunoglobulin (Ig) superfamily. These landmark discoveries, along with technical advances, ushered in a new era of discovery (de Wit and Ghosh, 2016).…”

Section: Families Of Recognition Molecules Underlie Synaptic Specificitymentioning

confidence: 99%

Synaptic Specificity, Recognition Molecules, and Assembly of Neural Circuits

Sanes

Zipursky

2020

Cell

261

196

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Developing neurons connect in specific and stereotyped ways to form the complex circuits that underlie brain function. By comparison to earlier steps in neural development, progress has been slow in identifying the cell surface recognition molecules that mediate these synaptic choices, but new high-throughput imaging, genetic, and molecular methods are accelerating progress. Over the past decade, numerous large and small gene families have been implicated in target recognition, including members of the immunoglobulin, cadherin, and leucine-rich repeat superfamilies. We review these advances and propose ways in which combinatorial use of multifunctional recognition molecules enables the complex neuron-neuron interactions that underlie synaptic specificity.

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Section: Families Of Recognition Molecules Underlie Synaptic Specificitymentioning

confidence: 99%

Synaptic Specificity, Recognition Molecules, and Assembly of Neural Circuits

Sanes

Zipursky

2020

Cell

261

196

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“…The antigen that mediates the blocking activity of this immune serum is a glycoprotein present on granule neurons, but not on PC12 cell membranes (Edmondson et al, 1985). Other antisera raised against the neuronal antigens NILE (Salton et al, 1983), Ll (Lindner et al, 1985), Ng-CAM (Grumet and Edelman, 1984), BSP-2 (Langley et al, 1983), and N-CAM (Thiery et al, 1977) do not disrupt neuron&a relationships, block neuronal migration or affect astroglial morphology in this microculture system (Edmondson et al, 1985). Since gliophilic neuronal migration in other regions of the developing weaver mouse brain is normal (Caviness and Rakic, 1978), it will be of interest to determine whether neuron-glia interaction molecules are distinct in different brain regions.…”

Section: Discussionmentioning

confidence: 99%

Weaver mouse cerebellar granule neurons fail to migrate on wild-type astroglial processes in vitro

Hatten¹,

Liem²,

Mason³

1986

J. Neurosci.

136

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To study the regulation of glial-guided neuronal migration, we have analyzed the behavior of cerebellar granule neurons purified from the homozygous weaver (wv/wv) B6CBA-w mouse, an autosomal recessive genetic mutation that suffers a failure of granule cell migration along Bergmann glial processes (Rakic and Sidman, 1973a, b; Rezai and Yoon, 1972), on the processes of astroglia purified from homozygous normal B6CBA-Aw-J-wv (+/+) mouse cerebella. When co-cultured with normal astroglia, weaver granule neurons failed to form neuron-glia contacts characteristic of migrating neurons and impaired normal astroglial morphological differentiation. Normal astroglial cells co-cultured with weaver granule cells had enlarged cell somata with stunted processes and enlarged endfeet compared to normal astroglia co-cultured with normal granule cells. In contrast, normal neurons associated with weaver astroglia, forming tight appositions seen for migrating neurons in vivo, and enhanced weaver astroglial morphological differentiation. Weaver astroglia co-cultured with normal granule cells contained a more normal complement of glial filaments and had a smaller perikaryon with longer, more tapered processes than their counterparts co-cultured with weaver neurons. These results suggest, in agreement with the study of Goldowitz and Mullen (1982) on heterozygous mutant chimeras, that the granule neuron is a primary site of action of the weaver gene, and further support our previous findings that neuron-glia interactions regulate astroglial morphological differentiation (Hatten, 1985).

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“…The cultures were then incubated in and mounted in DABCO. For double monolayer experiments, 1.5 ϫ primary antibody in 1% NGS overnight at 4ЊC: TAG-1 (1:1, provided 10 6 P6 EGL cells were added 30 min after the labeled rhombic lip by J. Dodd and T. Jessell) (Dodd et al, 1988); NCAM (1:100, Sigma) (Thiery et al, 1977); GFAP (1:1000, Dako) (Hatten and Liem, 1981); cells were plated onto the first monolayer.…”

Section: Immunocytochemistry Of Cellular Markers and Coculture Of Rhombic Lip Cells With Other Cell Types Markers Of Granule Cell Differementioning

confidence: 99%

Embryonic Precursor Cells from the Rhombic Lip Are Specified to a Cerebellar Granule Neuron Identity

Alder

Cho

Hatten

1996

Neuron

213

141

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The specification of diverse classes of neurons is critical to the development of the cerebellar cortex. Here, we describe the purification of early embryonic precursors of cerebellar granule neurons from the rhombic lip, the dorsal aspect of the midbrain/hindbrain region. Isolation of rhombic lip cells reveals a homogenous population of precursor cells that express general neuronal markers and the granule cell marker RU49, but fail to extend neurites or express differentiation markers. Differentiation is induced by coculture with external germinal layer (EGL) cells, or their membranes, suggesting that a local inducing factor acts after formation of the EGL. Thus, proliferating precursors within the rhombic lip are specified to be granule cells very early, with the availability of an inducing factor increasing over the course of development.

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Adhesion among neural cells of the chick embryo. II. Purification and characterization of a cell adhesion molecule from neural retina.

Cited by 405 publications

References 10 publications

Synaptic Specificity, Recognition Molecules, and Assembly of Neural Circuits

Synaptic Specificity, Recognition Molecules, and Assembly of Neural Circuits

Weaver mouse cerebellar granule neurons fail to migrate on wild-type astroglial processes in vitro

Embryonic Precursor Cells from the Rhombic Lip Are Specified to a Cerebellar Granule Neuron Identity

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