Adhesion and aggregation properties of probiotic and pathogen strains

Collado, María Carmen; Meriluoto, Jussi; Salminen, Seppo

doi:10.1007/s00217-007-0632-x

Cited by 467 publications

(392 citation statements)

References 29 publications

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“…rhamnosus GG and Lb. farciminis 103136 or to the pathogens tested, and this is consistent with previous studies (Collado et al, 2007(Collado et al, , 2008. Our data suggest that autoaggregation of Lb.…”

Section: Discussionsupporting

confidence: 93%

“…farciminis 3699 involves carbohydrate-lectin interactions and/or proteinaceous components present on the surface. Autoaggregation is a known property of some Lactobacillus isolates (Collado et al, 2007(Collado et al, , 2008 and allows the formation of biofilm in the gastrointestinal tract which contributes, as a physico-chemical barrier, to the preservation of the intestinal wall, along with the stimulation of the immune system (Jankovic et al, 2003;Castagliuolo et al, 2005;Voltan et al, 2007;Turpin et al, 2010), and to protection against colonization by pathogenic bacteria (Collado et al, 2007(Collado et al, , 2008. Furthermore, bacteria with a high autoaggregation potential will more easily colonize the intestinal and urogenital tracts (Boris et al, 1998;Cesena et al, 2001;Collado et al, 2005Collado et al, , 2007Collado et al, , 2008.…”

Section: Discussionmentioning

confidence: 99%

“…The ability of bacteria to autoaggregate and coaggregate was assessed according to the method described by Collado et al (2008) and expressed as a percentage of the total number of bacteria present. Briefly, stationary-phase cells from batch cultures were centrifuged, washed with PBS and resuspended in PBS to OD 600 0.3±0.05 (equivalent to 10 8 c.f.u.ml 21 ).…”

Section: Methodsmentioning

confidence: 99%

“…Several workers have suggested that the ability of beneficial micro-organisms to aggregate and adhere aids in colonization of the gut and in the establishment of a barrier which prevents enteropathogens from establishing an infection. Factors which prevent infection include the physical presence of beneficial micro-organisms and modulation of the gut immune system by these organisms (Cesena et al, 2001;Voltan et al, 2007;Collado et al, 2008;Sherman et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Tareb

Bernardeau²,

Guéguen

et al. 2013

Journal of Medical Microbiology

113

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Bacterial aggregation and/or adhesion are key factors for colonization of the digestive ecosystem and the ability of probiotic strains to exclude pathogens. In the present study, two probiotic strains, Lactobacillus rhamnosus CNCM-I-3698 and Lactobacillus farciminis CNCM-I-3699, were evaluated as viable or heat-killed forms and compared with probiotic reference Lactobacillus strains (Lb. rhamnosus GG and Lb. farciminis CIP 103136). The autoaggregation potential of both forms was higher than that of reference strains and twice that of pathogenic strains. The coaggregation potential of these two beneficial micro-organisms was evaluated against several pathogenic agents that threaten the global safety of the feed/food chain: Escherichia coli, Salmonella spp., Campylobacter spp. and Listeria monocytogenes. The strongest coaggregative interactions were demonstrated with Campylobacter spp. by a coaggregation test, confirmed by electron microscopic examination for the two forms. Viable forms were investigated for the nature of the bacterial cell-surface molecules involved, by sugar reversal tests and chemical and enzymic pretreatments. The results suggest that the coaggregation between both probiotic strains and C. jejuni CIP 70.2 T is mediated by a carbohydrate-lectin interaction. The autoaggregation potential of the two probiotics decreased upon exposure to proteinase, SDS or LiCl, showing that proteinaceous components on the surface of the two lactobacilli play an important role in this interaction. Adhesion abilities of both Lactobacillus strains were also demonstrated at significant levels on Caco-2 cells, mucin and extracellular matrix material. Both viable and heat-killed forms of the two probiotic lactobacilli inhibited the attachment of C. jejuni CIP 70.2 T to mucin. In conclusion, in vitro assays showed that Lb. rhamnosus CNCM-I-3698 and Lb. farciminis CNCM-I-3699, as viable or heat-killed forms, are adherent to different intestinal matrix models and are highly aggregative in vitro with pathogens, especially Campylobacter spp., the most commonly reported zoonotic agent in the European Union. This study supports the need for further in vivo investigations to demonstrate the potential food safety benefits of Lb. rhamnosus CNCM-I-3698 and Lb. farciminis CNCM-I-3699, live or heat-killed, in the global feed/food chain.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Tareb

Bernardeau²,

Guéguen

et al. 2013

Journal of Medical Microbiology

113

View full text Add to dashboard Cite

show abstract

“…Adherence to mucosal surfaces by probiotic microorganisms is an important ability for the colonisation of the human gastrointestinal system, which prevents their elimination by peristalsis and provides a competitive advantage over pathogens (Rivera-Espinoza et al 2010). Self-aggregation seems to be required for the adhesion of probiotic strains to intestinal epithelial cells and the ability to co-aggregate can form a barrier that prevents colonisation by the pathogens (Collado et al 2008). Due to the importance of elucidating the adhesion ability of P. acidilactici B14 in intestinal epithelial cells, it was considered relevant to assess the properties of self-aggregation and coaggregation of this strain.…”

Section: Resultsmentioning

confidence: 99%