Adhesion and Colonization of the Probiotic Lactobacillus rhamnosus Labeled by Dsred2 in Mouse Gut

Li, Chen; Bei, Tingting; Niu, Zhihua; Guo, Xin; Wang, Miaoshu; Lu, Haiquan; Gu, Xiaosong; Tian, Hongtao

doi:10.1007/s00284-019-01706-8

Cited by 18 publications

(7 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the persistence patterns of L. plantarum RS-09 in the small intestine (duodenum, jejunum and ileum) and the large intestine (cecum, colon and rectum) were different. This result was consistent with another study that treated mice with the probiotic Lacticaseibacillus rhamnosus , where the number of colonization strains increased from the proximal to the distal small intestine (duodenum < jejunum < ileum) ( Li et al, 2019 ). The persistence rate of L. plantarum in the large intestine was higher than that in the small intestine.…”

Section: Discussionsupporting

confidence: 91%

Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract

Zhao

Yang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

The beneficial effects of the probiotic strain Lactiplantibacillus plantarum (formerly Lactobacillus plantarum) are based on its adherence and colonization ability in the gut. However, little is known about the migration and long-term gut colonization of the strain. This study evaluated the gut colonization modes of Lactiplantibacillus plantarum RS-09 to identify the strain with long-term gut colonization potential. We established CFDA/SE-labeled RS-09 to study the temporal and spatial distribution of RS-09 in the intestine as well as to analyze its persistence in different parts of the intestine by flow cytometry. This study has shown that the RS-09 strain maintains strong adhesion abilities under acid (pH 2.5) and base (pH 8.5) conditions. In addition, CFDA/SE can be used as an indicator for the labeling of L. plantarum RS-09 in the intestinal tract in vivo. We established a growth kinetics model of RS-09 to elucidate its persistence in the intestine. In vivo persistence experiments showed that the persistence rate of RS-09 was the highest in the cecum (69.5%) and the lowest in the duodenum (12.8%) at 96 h. After 20 days, RS-09 was predominantly localized in the cecum and colon steadily. These studies provide new insights into the long-term persistence of L. plantarum in the gastrointestinal tract. The CFDA/SE label system may be used to study the in vivo colonization dynamics of other probiotic strains.

show abstract

Section: Discussionsupporting

confidence: 91%

Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract

Zhao

Yang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…This study used oligosaccharides commonly found in dairy products as emerging prebiotics. This study was based on high-throughput sequencing results, and red fluorescent protein-labeled L. rhamnosus was constructed according to the method described by Li et al (2019). The activated labeled L. rhamnosus was inoculated with erythromycin (1 μg/mL) in an amount of 1% inoculum, and XOS and IMO (0.4, 0.8, 1.2, 1.6, and 2.0% each), FOS (1.2, 1.6, 2.0, 2.4, and 2.8%), and inulin (0.2, 0.4, 0.6, and 0.8%) of the basal medium, respectively, were added and cultured at 37°C.…”

Section: In Vitro Screening Of Synbioticsmentioning

confidence: 99%

Probiotics, prebiotics, and synbiotics regulate the intestinal microbiota differentially and restore the relative abundance of specific gut microorganisms

Niu

Zou

et al. 2020

Journal of Dairy Science

Self Cite

View full text Add to dashboard Cite

Fermented milk is an effective carrier for probiotics, the consumption of which improves host health. The beneficial effects of probiotics, prebiotics, and synbiotics on gut dysbiosis have been reported previously. However, the way in which specific probiotics, prebiotics, and synbiotics regulate intestinal microbes remains unclear. Therefore, the probiotics Lactobacillus rhamnosus AS 1.2466 and Lactobacillus delbrueckii ssp. bulgaricus ATCC 11842 and the prebiotics xylooligosaccharide and red ginseng extracts were fed to mice to determine their effects on the intestinal microbiota. Then, mice were administered xylooligosaccharide and L. rhamnosus (synthesis) by gavage, and the number of L. rhamnosus was determined in the intestine at different times. The results show that probiotics and prebiotics can quickly reduce the Firmicutes/Bacteroidetes ratio, inhibit harmful bacteria (such as Klebsiella and Escherichia coli), and accelerate the recovery of beneficial intestinal microorganisms (such as Lactobacillus). In a complex intestinal microecology, different probiotics and prebiotics have different effects on specific intestinal microorganisms that cannot be recovered in the short term. In addition, after 20 d of intragastric xylooligosaccharide addition at 0.12 g/kg of body weight, L. rhamnosus colonization in the mouse ileum was 7.48 log cfu/mL, which was higher than in the lowdose group, prolonging colonization time and increasing the number of probiotics in the intestine. Therefore, this study demonstrated that probiotics and prebiot-ics can promote the balance of intestinal microbiota by regulating specific microbes in the intestine, and the effects of a suitable combination of synbiotics are beneficial, laying the foundation for the development of new dairy products rich in synbiotics.

show abstract

“…), coupled with the survival through the hostile gastrointestinal tract (GIT) environment (acidic pH, bile salts, digestive enzymes, etc.) and the ability to colonize the gut, are the major challenges ( Li et al, 2019a ). However, microencapsulation seems to be a highly productive and holistic approach to overcome this problem ( Feng et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Therapeutic, Prophylactic, and Functional Use of Probiotics: A Current Perspective

2020

View full text Add to dashboard Cite

Probiotics are considered as the twenty-first century panpharmacon due to their competent remedial power to cure from gastrointestinal dysbiosis, systematic metabolic diseases, and genetic impairments up to complicated neurodegenerative disorders. They paved the way for an innovative managing of various severe diseases through palatable food products. The probiotics’ role as a “bio-therapy” increased their significance in food and medicine due to many competitive advantages over traditional treatment therapies. Their prophylactic and therapeutic potential has been assessed through hundreds of preclinical and clinical studies. In addition, the food industry employs probiotics as functional and nutraceutical ingredients to enhance the added value of food product in terms of increased health benefits. However, regardless of promising health-boosting effects, the probiotics’ efficacy still needs an in-depth understanding of systematic mechanisms and factors supporting the healthy actions.

show abstract

Adhesion and Colonization of the Probiotic Lactobacillus rhamnosus Labeled by Dsred2 in Mouse Gut

Cited by 18 publications

References 21 publications

Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract

Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract

Probiotics, prebiotics, and synbiotics regulate the intestinal microbiota differentially and restore the relative abundance of specific gut microorganisms

Therapeutic, Prophylactic, and Functional Use of Probiotics: A Current Perspective

Contact Info

Product

Resources

About