Adhesion and Proliferation of Human Periodontal Ligament Cells on Poly(2-methoxyethyl acrylate)

Abstract

Human periodontal ligament (PDL) cells obtained from extracted teeth are a potential cell source for tissue engineering. We previously reported that poly(2-methoxyethyl acrylate) (PMEA) is highly biocompatible with human blood cells. In this study, we investigated the adhesion, morphology, and proliferation of PDL cells on PMEA and other types of polymers to design an appropriate scaffold for tissue engineering. PDL cells adhered and proliferated on all investigated polymer surfaces except for poly(2-hydroxyethyl methacrylate) and poly[(2-methacryloyloxyethyl phosphorylcholine)-co-(n-butyl methacrylate)]. The initial adhesion of the PDL cells on PMEA was comparable with that on polyethylene terephthalate (PET). In addition, the PDL cells on PMEA spread well and exhibited proliferation behavior similar to that observed on PET. In contrast, platelets hardly adhered to PMEA. PMEA is therefore expected to be an excellent scaffold for tissue engineering and for culturing tissue-derived cells in a blood-rich environment.

Design of Polymeric Biomaterials: The “Intermediate Water Concept”

“…The intermediate water plays a significant role in blood compatibility. Recently, we have reported that endothelial cells, 130,131 smooth muscle cells, 130 periodontal ligament cells, 132 and tumor cells, 109,116,117,133 but not platelets can attach to PMEA and its analogous polymers. Also, we revealed that tumor cell adhesion and morphology on these polymers are affected by protein adsorption behavior such as fibronectin.…”

Fabrication of polymeric biomaterials: a strategy for tissue engineering and medical devices

“…112 Another example is the Gecko feet which consists of about 5 Â 10 5 setae and can generate a strong adhesive force which has drawn significant research interest. 113,114 Inspired by the 3D pattern existing in biological structures, polymer and biomaterial scientists have developed 3D hierarchical and sophisticated architecture in the order of micron-to nano-structure from functional polymers and biomaterials [112][113][114][115][116][117][118][119][120][121][122][123][124][125] alternative to the existing lithography techniques.…”

Novel Tough and Transparent Ultra-Extensible Nanocomposite Elastomers Based on Poly(2-methoxyethylacrylate) and Their Switching between Plasto-Elasticity and Viscoelasticity

“…Additionally, the same materials could play analogous bio-inspired roles in robotic applications. Related to the mentioned implant or artificial-tissue-applications is the potential of polyMEA as scaffold in tissue engineering [ 55 , 56 ]. The high transparency of the studied polyMEA nanocomposites is a further valuable property, both for some biomedical (e.g., sensors), as well as for technical applications.…”