Adhesion and Proliferation of Osteoblast-Like Cells on Porous Polyetherimide Scaffolds

Zhang, Yanbo; Li, Ruiyan; Wu, Wenzheng; Qing, Yun’an; Tang, Xiongfeng; Ye, Wenli; Zhang, Zhiyong; Qin, Yanguo

doi:10.1155/2018/1491028

Cited by 12 publications

(10 citation statements)

References 22 publications

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“…Short-term cell adhesion can be quantified by cell viability (Figure 6A). Cells were allowed to adhere for 2 h before quantification was performed (similar to a previous study, Zhang et al, 2018). The number of adherent cells on TC4p was higher than those on the CaP-p and Sr-CaP-p groups, which had nearly identical OD values (which represent the relative cell number).…”

Section: Cell Adhesionmentioning

confidence: 70%

Improved Osteogenesis of Selective-Laser-Melted Titanium Alloy by Coating Strontium-Doped Phosphate With High-Efficiency Air-Plasma Treatment

Xing

Wei

et al. 2020

Front. Bioeng. Biotechnol.

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Surface treatment and bioactive metal ion incorporation are effective methods for the modification of titanium alloys to be used as biomaterials. However, few studies have demonstrated the use of air-plasma treatment in orthopedic biomaterial development. Additionally, no study has performed a direct comparison between unmodified titanium alloys and air-plasma-treated alloys with respect to their biocompatibility and osteogenesis. In this study, the biological activities of unmodified titanium alloys, air-plasma-treated titanium alloys, and air-plasma-treated strontium-doped/undoped calcium phosphate (CaP) coatings were compared. The strontium-doped CaP (Sr-CaP) coating on titanium alloys were produced by selective laser melting (SLM) technology as well as micro-arc oxidation (MAO) and air-plasma treatment. The results revealed that rapid air-plasma treatment improved the biocompatibility of titanium alloys and that Sr-CaP coating together with air-plasma treatment significantly enhanced both the biocompatibility and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Overall, this study demonstrated that low temperature air-plasma treatment is a fast and effective surface modification which improves the biocompatibility of titanium alloys. Additionally, air-plasma-treated Sr-CaP coatings have numerous practical applications and may provide researchers with new tools to assist in the development of orthopedic implants.

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Section: Cell Adhesionmentioning

confidence: 70%

Improved Osteogenesis of Selective-Laser-Melted Titanium Alloy by Coating Strontium-Doped Phosphate With High-Efficiency Air-Plasma Treatment

Xing

Wei

et al. 2020

Front. Bioeng. Biotechnol.

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“…While the limited interfacial area of scaffolds with large pores can inhibit cell adhesion, they can play a crucial role in the proliferation and migration of the cells into the scaffold. 9 As such, cell infiltration may be restricted by pore size reduction due to greater surface area to volume ratio. Therefore, designing a scaffold architecture that has a sufficiently high surface area to promote cell adhesion but with a pore size optimal for cell proliferation and infiltration could favorably alter cell behavior.…”

Section: Discussionmentioning

confidence: 99%

“…8 In contrast, larger pore sizes (500 μm) did not enhance cell attachment or promote cell infiltration. 9,10 Similarly, a scaffold pore size below 100 μm was associated with fibroblast differentiation and nonmineralized soft tissue infiltration. 11 Apart from pore size, 3D-printed scaffolds with different strut offsets, particularly those with values of 50% and 100%, can allow cells to grow homogeneously due to a higher number of anchorage points for attachment, while interconnected struts create the angles which can influence cell function through mechanotransduction.…”

Section: Introductionmentioning

confidence: 99%

Effects of Gradient and Offset Architectures on the Mechanical and Biological Properties of 3-D Melt Electrowritten (MEW) Scaffolds

Abbasi

Abdal‐hay

Hamlet

et al. 2019

ACS Biomater. Sci. Eng.

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This study describes the fabrication and characterization of three-dimensional (3-D) poly(ε-caprolactone) (PCL) scaffolds with defined pore architectures prepared using the melt electrowriting (MEW) technique. Three homogeneous pore-sized (250, 500, and 750 μm) scaffolds, two fiber offset (30/70% and 50/50%), and a three-layered (250 μm bottom–500 μm middle–750 μm top) gradient pore-sized scaffolds were designed and printed with ∼10 μm fibers. The mechanical properties (tensile and compression tests), total surface area, porosity of these scaffolds, and their ability to promote the attachment and proliferation of human osteoblasts were then compared. All scaffolds induced good tensile properties; however, they reacted differently during compressive testing. The offset 30/70 scaffold had the highest surface area to volume ratio which enhanced osteoblast attachment after 3 days of cell culture. While the highest initial level of osteoblast attachment at day 1 was found on the 250 μm homogeneous scaffold, the highest degree of cell proliferation and infiltration at day 30 was observed in the three-layered graded porosity scaffold. In terms of physical and biological properties to support bone cell distribution and migration through the entire structure of the scaffold, our results suggest that melt electrowritten offset and gradient scaffolds are good candidate platforms for cell infiltration and growth compared to homogeneous scaffolds.

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“…After seeding the cells on the samples for 2 h, quantification was performed to measure the cell attachment according to a previous study ( Zhang et al, 2018 ) ( Figure 3C ). Notably, the bilayer coatings facilitated the cells adhering on the substrate rather than the bare PLLA film.…”

Section: Resultsmentioning

confidence: 99%

Improved Osteogenesis by Mineralization Combined With Double-Crosslinked Hydrogel Coating for Proliferation and Differentiation of Mesenchymal Stem Cells

You

Cao

et al. 2021

Front. Bioeng. Biotechnol.

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In consideration of improving the interface problems of poly-L-lactic acid (PLLA) that hindered biomedical use, surface coatings have been explored as an appealing strategy in establishing a multi-functional coating for osteogenesis. Though the layer-by-layer (LBL) coating developed, a few studies have applied double-crosslinked hydrogels in this technique. In this research, we established a bilayer coating with double-crosslinked hydrogels [alginate–gelatin methacrylate (GelMA)] containing bone morphogenic protein (BMP)-2 [alginate-GelMA/hydroxyapatite (HA)/BMP-2], which displayed great biocompatibility and osteogenesis. The characterization of the coating showed improved properties and enhanced wettability of the native PLLA. To evaluate the biosafety and inductive ability of osteogenesis, the behavior (viability, adherence, and proliferation) and morphology of human bone mesenchymal stem cells (hBMSCs) on the bilayer coatings were tested by multiple exams. The satisfactory function of osteogenesis was verified in bilayer coatings. We found the best ratios between GelMA and alginate for biological applications. The Alg70-Gel30 and Alg50-Gel50 groups facilitated the osteogenic transformation of hBMSCs. In brief, alginate-GelMA/HA/BMP-2 could increase the hBMSCs’ early transformation of osteoblast lineage and promote the osteogenesis of bone defect, especially the outer hydrogel layer such as Alg70-Gel30 and Alg50-Gel50.

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Adhesion and Proliferation of Osteoblast-Like Cells on Porous Polyetherimide Scaffolds

Cited by 12 publications

References 22 publications

Improved Osteogenesis of Selective-Laser-Melted Titanium Alloy by Coating Strontium-Doped Phosphate With High-Efficiency Air-Plasma Treatment

Improved Osteogenesis of Selective-Laser-Melted Titanium Alloy by Coating Strontium-Doped Phosphate With High-Efficiency Air-Plasma Treatment

Effects of Gradient and Offset Architectures on the Mechanical and Biological Properties of 3-D Melt Electrowritten (MEW) Scaffolds

Improved Osteogenesis by Mineralization Combined With Double-Crosslinked Hydrogel Coating for Proliferation and Differentiation of Mesenchymal Stem Cells

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