Adhesion and proliferation of skeletal muscle cells on single layer poly(lactic acid) ultra-thin films

Ricotti, Leonardo; Taccola, Silvia; Pensabene, Virginia; Mattoli, Virgilio; Fujie, Toshinori; Takeoka, Shinji; Menciassi, Arianna; Dario, P.

doi:10.1007/s10544-010-9435-0

Cited by 50 publications

(49 citation statements)

References 33 publications

(30 reference statements)

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“…Characterization of the nanofilm by atomic force microscopy confirmed previously published data for similar films (9,10,11), showing thickness of the PLLA film of 120±25 nm and average roughness RA below 10 nm. Next, to test the ability of the nanofilm to repair uterine defects, we developed a modified version of the device described by Perrini and colleagues (12).…”

Section: Adhesive and Sealing Performance On Human Chor Ion-amnionsupporting

confidence: 85%

Repairing Fetal Membranes with a Self-adhesive Ultrathin Polymeric Film: Evaluation in Mid-gestational Rabbit Model

et al. 2014

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Preterm premature rupture of membranes (PPROM) causes 40% of all preterm births, affecting 150,000 women each year in the United States. Prenatal diagnostic procedures and surgical interventions increase incidence of adverse events, leading to iatrogenic membrane rupture after a fetoscopic procedure in 45% of cases. We propose an ultrathin, self-adherent, poly-L-lactic acid patch ("nanofilm") as a reparative wound closure after endoscopic/fetoscopic procedures. These nanofilms are compatible with application in wet conditions and with minimally invasive instrumentation.Ex vivo studies to evaluate the nanofilm were conducted using human chorion-amnion (CA) membranes. A custom-built inflation device was used for mechanical characterization of CA membranes and for assessment of nanofilm adhesion and sealing of membrane defects up to 3 mm in size. These ex vivo tests demonstrated the ability of the nanofilm to seal human CA defects ranging in size from 1 to 3 mm in diameter. In vivo survival studies were conducted in 25 mid-gestational rabbits, defects were created by perforating the uterus and the CA membranes and subsequently using the nanofilm to seal these wounds. These in vivo studies confirmed the successful sealing of defects smaller than 3 mm observed ex vivo.Histological analysis of whole harvested uteri 7 days after surgery showed intact uterine walls in 59% of the nanofilm repaired fetuses, along with increased uterine size and intrauterine development in 63% of the cases. In summary, we have developed an ultrathin, self-adhesive nanofilm for repair of uterine membrane defects.

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Section: Adhesive and Sealing Performance On Human Chor Ion-amnionsupporting

confidence: 85%

Repairing Fetal Membranes with a Self-adhesive Ultrathin Polymeric Film: Evaluation in Mid-gestational Rabbit Model

et al. 2014

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“…However, most groups have focused on only one or two of the numerous aspects affecting the development of muscle constructs, such as micro/nano-topography [8], [14], [26], [27], [28], [29], [30], matrix stiffness [16], [31], [32], [33], chemical cues [34], [35] and electrical stimulation [17], [36], [37], [38]. The combination of multiple mechanical, electrical, topographical and chemical cues constitutes an active field of research and it holds great promises for the commitment of stem cells towards specific cell phenotypes [39], but also for the late differentiation of skeletal muscle cells [40].…”

Section: Introductionmentioning

confidence: 99%

Boron Nitride Nanotube-Mediated Stimulation of Cell Co-Culture on Micro-Engineered Hydrogels

et al. 2013

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In this paper, we describe the effects of the combination of topographical, mechanical, chemical and intracellular electrical stimuli on a co-culture of fibroblasts and skeletal muscle cells. The co-culture was anisotropically grown onto an engineered micro-grooved (10 µm-wide grooves) polyacrylamide substrate, showing a precisely tuned Young’s modulus (∼ 14 kPa) and a small thickness (∼ 12 µm). We enhanced the co-culture properties through intracellular stimulation produced by piezoelectric nanostructures (i.e., boron nitride nanotubes) activated by ultrasounds, thus exploiting the ability of boron nitride nanotubes to convert outer mechanical waves (such as ultrasounds) in intracellular electrical stimuli, by exploiting the direct piezoelectric effect. We demonstrated that nanotubes were internalized by muscle cells and localized in both early and late endosomes, while they were not internalized by the underneath fibroblast layer. Muscle cell differentiation benefited from the synergic combination of topographical, mechanical, chemical and nanoparticle-based stimuli, showing good myotube development and alignment towards a preferential direction, as well as high expression of genes encoding key proteins for muscle contraction (i.e., actin and myosin). We also clarified the possible role of fibroblasts in this process, highlighting their response to the above mentioned physical stimuli in terms of gene expression and cytokine production. Finally, calcium imaging-based experiments demonstrated a higher functionality of the stimulated co-cultures.

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“…55-58 The chemical biocompatibility of PLLA nanofilms has been widely characterized in vitro with different cell types 12,16 , and assessed with in vivo animal model 8,41,55 .…”

Section: Device Assemblymentioning

confidence: 99%

Ultrathin Polymer Membranes with Patterned, Micrometric Pores for Organs-on-Chips

Costa

Terekhov

Gnecco³

et al. 2016

ACS Appl. Mater. Interfaces

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The basal lamina or basement membrane (BM) is a key physiological system that participates in physicochemical signaling between tissue types. Its formation and function are essential in tissue maintenance, growth, angiogenesis, disease progression, and immunology. In vitro models of the BM, e.g., Boyden and transwell chambers, are common in cell biology and lab-on-a-chip devices where cells require apical and basolateral polarization. Extravasation, intravasation, membrane transport of chemokines, cytokines, chemotaxis of cells, and other key functions are routinely studied in these models. The goal of the present study was to integrate a semipermeable ultrathin polymer membrane with precisely positioned pores of 2 μm diameter in a microfluidic device with apical and basolateral chambers. We selected poly (L-lactic acid) (PLLA), a transparent biocompatible polymer, to prepare the semipermeable ultrathin membranes. The pores were generated by pattern transfer using a three-step method coupling femtosecond laser machining, polymer replication, and spin coating. Each step of the fabrication process was characterized by scanning electron microscopy to investigate reliability of the process and fidelity of pattern transfer. In order to evaluate the compatibility of the fabrication method with organs-on-a-chip technology, porous PLLA membranes were embedded in polydimethylsiloxane (PDMS) microfluidic devices and used to grow human umbilical vein endothelial cells (HUVECS) on top of the membrane with perfusion through the basolateral chamber. Viability of cells, optical transparency of membranes and strong adhesion of PLLA to PDMS were observed, thus confirming the suitability of the prepared membranes for use in organs-on-a-chip devices.

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Adhesion and proliferation of skeletal muscle cells on single layer poly(lactic acid) ultra-thin films

Cited by 50 publications

References 33 publications

Repairing Fetal Membranes with a Self-adhesive Ultrathin Polymeric Film: Evaluation in Mid-gestational Rabbit Model

Repairing Fetal Membranes with a Self-adhesive Ultrathin Polymeric Film: Evaluation in Mid-gestational Rabbit Model

Boron Nitride Nanotube-Mediated Stimulation of Cell Co-Culture on Micro-Engineered Hydrogels

Ultrathin Polymer Membranes with Patterned, Micrometric Pores for Organs-on-Chips

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