Adhesion complexes implicated in intestinal epithelial cell‐matrix interactions

Stutzmann, J; Bellissent‐Waydelich, Anne; Fontao, Lionel; Launay, Jean-François; Simon‐Assmann, Patricia

doi:10.1002/1097-0029(20001015)51:2<179::aid-jemt9>3.3.co;2-w

Cited by 6 publications

(7 citation statements)

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“…Cell-cell/cell-matrix interactions. The role of interaction between integrins and extracellular matrix during enterocyte differentiation is well characterized (4,54) and involves the coordinated production of proteins from both the intestinal epithelium and mesenchymal cells (46). Our data suggest that distinct families of genes involved in cell-cell or cell-matrix interactions are expressed in proliferating as opposed to postproliferative or differentiated cells.…”

Section: Discussionmentioning

confidence: 70%

Gene expression profiling of Caco-2 BBe cells suggests a role for specific signaling pathways during intestinal differentiation

Fleet

Wang

Vitek

et al. 2003

Physiological Genomics

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We examined the pattern of gene expression resulting from spontaneous differentiation of Caco-2 BBe cells to gain insight into the molecular changes necessary for enterocyte differentiation. RNA was prepared from cells harvested at three cell stages: proliferating (50% confluent, 2 days in culture), postproliferative nondifferentiated (8 days), and differentiated (15 days). Gene expression profiles were determined using Affymetrix Human Genome U95A GeneChips. Differentially expressed genes were identified following statistical analysis (i.e., ANOVA, bootstrapping adjustments to P values, false detection rate criterion). We identified 1,150 unique genes as differentially expressed; expression of 48.6% fell and 46% increased from 2 to 15 days, while 5.4% had expression that either peaked or dipped at 8 days. Genes expressed during differentiation included several small-intestine-specific genes involved in nutrient transport/metabolism, e.g., DCT1, hephaestin, folate receptor 1, sucrase-isomaltase, and apolipoproteins CI, CIII, B100, H, and M, indicating that this colonic adenocarcinoma cell line has a hybrid colonocyte/enterocyte phenotype. Patterns of gene expression based upon functional classification suggest a role for cell-cell/cell-matrix interactions, suppression of Wnt signaling, and activation of TGFbeta and phosphatidylinositol 3-kinase pathways during enterocyte differentiation.

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Section: Discussionmentioning

confidence: 70%

Gene expression profiling of Caco-2 BBe cells suggests a role for specific signaling pathways during intestinal differentiation

Fleet

Wang

Vitek

et al. 2003

Physiological Genomics

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“…42 Moreover, the integrin a5b1 is expressed by both IGR39 and HT1080 cells. 41 It is also worth pointing out, that HT29-D4 cells, that do not express a5b1 integrin, 18,43 are very little sensitive to lebectin. The identification of the specific integrin(s) involved in the effects of lebectin on tumour cells behaviour is currently under investigation.…”

Section: Discussionmentioning

confidence: 99%

Lebectin, a novel C-type lectin from Macrovipera lebetina venom, inhibits integrin-mediated adhesion, migration and invasion of human tumour cells

Sarray

Berthet

Calvete

et al. 2004

Laboratory Investigation

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The adhesion receptors of the integrin family play an essential role during tumour progression and thus represent interesting potential targets for the development of new therapeutic agents. The snake venom contains natural inhibitors of integrin-ligand interactions called disintegrins. It also contains C-type lectin proteins mainly known as modulators of platelet aggregation. In this study, we demonstrate that lebectin, a novel C-type lectin isolated from Macrovipera lebetina venom, displayed an anti-integrin activity. Lebectin inhibited the integrin-mediated attachment of various tumour cell lines to different adhesion substrata. The C-type lectin also completely blocked cell migration towards fibronectin in haptotaxis assays and prevented invasion of fibrin gels by tumour cells. In addition, lebectin proved to be a potent inhibitor of tumour cell proliferation. Although the specific integrins affected by lebectin are not identified in this study, the integrin alpha 5 beta 1 might be involved.

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“…Cell lines present the same repertoire of genetic alterations as primary tumors (Gayet et al, 2001) and could be useful for relating biological and pharmacological changes with particular genetic alterations. Furthermore, they can contribute to the understanding of the dynamics of cell-extracellular matrix interactions involved in cell migration, proliferation, and tumorigenicity (Stutzmann et al, 2000). As an example, a link between laminin expression and tumor growth was reported using HT-29 cells.…”

Section: Human Tumor Colorectal Cell Lines Are Potent Models In Intesmentioning

confidence: 99%

In vitro models of intestinal epithelial cell differentiation

Turck²,

et al. 2006

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The intestinal epithelium is a particularly interesting tissue as (1) it is in a constant cell renewal from a stem cell pool located in the crypts which form, with the underlying fibroblasts, a stem cell niche and (2) the pluripotent stem cells give rise to four main cell types: enterocytes, mucus, endocrine, and Paneth cells. The mechanisms leading to the determination of phenotype commitment and cell-specific expressions are still poorly understood. Although transgenic mouse models are powerful tools for elucidating the molecular cascades implicated in these processes, cell culture approaches bring easy and elegant ways to study cellular behavior, cell interactions, and cell signaling pathways for example. In the present review, we will describe the major tissue culture technologies that allow differentiation of epithelial cells from undifferentiated embryonic or crypt cells. We will point to the necessity of the re-creation of a complex microenvironment that allows full differentiation process to occur. We will also summarize the characteristics and interesting properties of the cell lines established from human colorectal tumors.

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Adhesion complexes implicated in intestinal epithelial cell‐matrix interactions

Cited by 6 publications

References 0 publications

Gene expression profiling of Caco-2 BBe cells suggests a role for specific signaling pathways during intestinal differentiation

Gene expression profiling of Caco-2 BBe cells suggests a role for specific signaling pathways during intestinal differentiation

Lebectin, a novel C-type lectin from Macrovipera lebetina venom, inhibits integrin-mediated adhesion, migration and invasion of human tumour cells

In vitro models of intestinal epithelial cell differentiation

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