Adhesion dynamics of Flavobacterium columnare to channel catfish Ictalurus punctatus and zebrafish Danio rerio after immersion challenge

Olivares-Fuster, Óscar; Bullard, Stephen A.; McElwain, Andrew; Llosa, Maria Jose; Arias, Covadonga R.

doi:10.3354/dao02371

Cited by 68 publications

(65 citation statements)

References 21 publications

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“…Formation of biofilm under water flow occurred on a time scale (5 h for early attachment and more than 12 for microcolony formation) similar to that of biofilm formation under static conditions. This contrasts with the short time (5 to 30 min) required for the cells to attach to host tissues while the host is actively swimming (8,20). This time difference between in vivo and in vitro adhesion dynamics could be due to the positive chemotaxis toward host tissues that F. columnare displays (21).…”

Section: Discussionmentioning

confidence: 41%

“…Previous studies have focused on in vivo attachment to host tissues and showed that both gill and skin tissues can be rapidly colonized after exposure to the pathogen (19,20). However, significant differences were found between genomovars I and II in terms of both chemotaxis response and attachment to host tissues, with genomovar II showing a higher affinity for channel catfish tissues than genomovar I (20,21).…”

mentioning

confidence: 57%

“…Previous studies of F. columnare adhesion mechanisms focused on host-pathogen interactions by using in vivo models (20) or proxies such as explants of living tissues (19) or polystyrene plates (32). Herein, we have shown that F. columnare can colonize a variety of man-made materials and have characterized the progression of biofilm development on different surfaces.…”

Section: Discussionmentioning

confidence: 98%

“…This contrasts with what was observed under static conditions since both strains developed microcolonies and mature biofilm in similar time frames (data not shown for ARS-1). In a previous study, we examined the adhesion capability of ARS-1 and observed that although it was able to attach to host tissues at high levels (equal to those of virulent strain BGFS-27), it failed to persist in the host over time (20). The mechanisms used by F. columnare to attach to a host or to man-made surfaces are still unknown because of the difficulty of creating mutants of this species (36).…”

Section: Discussionmentioning

confidence: 99%

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Biofilm Formation by the Fish Pathogen Flavobacterium columnare: Development and Parameters Affecting Surface Attachment

Cai

Fuente

Arias

2013

Appl Environ Microbiol

102

105

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bFlavobacterium columnare is a bacterial fish pathogen that affects many freshwater species worldwide. The natural reservoir of this pathogen is unknown, but its resilience in closed aquaculture systems posits biofilm as the source of contagion for farmed fish. The objectives of this study were (i) to characterize the dynamics of biofilm formation and morphology under static and flow conditions and (ii) to evaluate the effects of temperature, pH, salinity, hardness, and carbohydrates on biofilm formation. Nineteen F. columnare strains, including representatives of all of the defined genetic groups (genomovars), were compared in this study. The structure of biofilm was characterized by light microscopy, confocal laser scanning microscopy, and scanning electron microscopy. F. columnare was able to attach to and colonize inert surfaces by producing biofilm. Surface colonization started within 6 h postinoculation, and microcolonies were observed within 24 h. Extracellular polysaccharide substances and water channels were observed in mature biofilms (24 to 48 h). A similar time course was observed when F. columnare formed biofilm in microfluidic chambers under flow conditions. The virulence potential of biofilm was confirmed by cutaneous inoculation of channel catfish fingerlings with mature biofilm. Several physicochemical parameters modulate attachment to surfaces, with the largest influence being exerted by hardness, salinity, and the presence of mannose. Maintenance of hardness and salinity values within certain ranges could prevent biofilm formation by F. columnare in aquaculture systems.

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Section: Discussionmentioning

confidence: 41%

mentioning

confidence: 57%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Biofilm Formation by the Fish Pathogen Flavobacterium columnare: Development and Parameters Affecting Surface Attachment

Cai

Fuente

Arias

2013

Appl Environ Microbiol

102

105

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“…25 Interestingly, F. columnare shows a strong chemotactic attraction to catfish mucus, where it appears to associate with goblet cells. [26][27][28] Mucin levels are also altered by short-term fasting, a nutritional state previously associated with higher F. columnare mortality. [29][30][31] Goblet cell proliferation appears to be under the control of anterior gradient 2 protein (AGR2) in zebrafish.…”

Section: Mucins In the Gillmentioning

confidence: 96%

Physiology and immunology of mucosal barriers in catfish (Ictalurus spp.)

et al. 2015

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The mucosal barriers of catfish (Ictalurus spp) constitute the first line of defense against pathogen invasion while simultaneously carrying out a diverse array of other critical physiological processes, includ\ing nutrient adsorption, osmoregulation, waste excretion, and environmental sensing. Catfish depend more heavily on mucosal barriers than their terrestrial counterparts as they are continuously interacting with the aquatic microbiota. Our understanding of these barriers, while growing, is still limited relative to that of mammalian model systems. Nevertheless, a combination of molecular and cellular studies in catfish over the last few decades, and particularly within the last few years, has helped to elucidate many of the primary actors and pathways critical to their mucosal health. Here we describe aspects of innate and adaptive immune responses in the primary mucosal tissues (skin, gill, and intestine) of catfish, focusing on mucus-driven responses, pathogen recognition, soluble mediators, and immunoglobulin and T-cell derived immunity. Modulation of mucosal barriers will be critical moving forward for crafting better diets, improving vaccine delivery, enhancing water quality, and ensuring sustainable production practices in catfish.

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Gut dysbiosis induced by florfenicol increases susceptibility to Aeromonas hydrophila infection in Zebrafish Danio rerio after the recommended withdrawal period

Burgos,

Cai,

Arias

2024

J Aqua Anim Hlth

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Florfenicol is a broad‐spectrum antibiotic approved by the US Food and Drug Administration (FDA) to treat both systemic and external bacterial infections in food fish. The objective of this study was to evaluate the effect of Florfenicol (FFC)‐medicated feed on the gut microbiota of zebrafish to determine i) if the therapeutic dose of FFC‐medicated feed induces dysbiosis, and ii) if fish with altered gut microbiota were more susceptible to subsequent infection by the common opportunistic fish pathogen Aeromonas hydrophila. We used 16S rRNA gene sequencing to analyze the gut microbiota of FFC‐medicated feed‐treated zebrafish, and our results found that FFC‐medicated feed induced disruption of the gut microbiota. Dysbiosis was observed in all treated groups with a significant increase in bacterial diversity, and it was characterized by a remarked bloom of Proteobacteria and a drastic decline of Mycoplasma and Cetobacterium in treated animals without noticeable clinical signs or mortalities. In addition, the increase of Proteobacteria wasn´t significantly reduced after the recommended 15‐day antibiotic‐withdrawal period, and the zebrafish treated with FFC‐medicated feed exhibited a significantly higher mortality rate when they were subsequently challenged with A. hydrophila compared to the regular feed groups. Interestingly, the most dramatic changes in the gut microbiome composition occurred at the transition time between the late stage of the medicated treatment and the beginning of the withdrawal period, instead of the time during the Aeromonas infection. To conclude, the administration of FFC‐medicated feed at the recommended dose induced gut dysbiosis in zebrafish, and it did not recover to the baseline after the recommended withdrawal period. Our findings suggest that the use of antibiotics in fish elicits a similar response to those previously described in mammals and possibly makes the host more susceptible to subsequent infections of opportunistic pathogens. This study using a controlled model system suggests that antibiotics in aquaculture may have long‐term effects on the general wellbeing of the fish.

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Adhesion dynamics of Flavobacterium columnare to channel catfish Ictalurus punctatus and zebrafish Danio rerio after immersion challenge

Cited by 68 publications

References 21 publications

Biofilm Formation by the Fish Pathogen Flavobacterium columnare: Development and Parameters Affecting Surface Attachment

Biofilm Formation by the Fish Pathogen Flavobacterium columnare: Development and Parameters Affecting Surface Attachment

Physiology and immunology of mucosal barriers in catfish (Ictalurus spp.)

Gut dysbiosis induced by florfenicol increases susceptibility to Aeromonas hydrophila infection in Zebrafish Danio rerio after the recommended withdrawal period

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