Adhesion mechanisms of human lens epithelial cells on 4 intraocular lens materials

Iersura, Piera; Torreggiani, Alberto; Cellini, Mauro; Caramazza, R

doi:10.1016/s0886-3350(99)80050-9

Cited by 54 publications

(34 citation statements)

References 26 publications

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“…On the hydrophobic acrylic and PMMA IOLs, the fi gure of mononuclear macrophage apposition at the 1-and 4-week time points ( fi g. 3 b) contrasts that of LEC outgrowth ( fi g. 3 a). This can be further supported by observations reporting that PMMA causes successful spread and proliferation of LECs in vitro [33,34] due to the formation of focal contacts and stress fi bers. In this study, we hypothesize that the minimal outgrowth of LECs on PMMA results from the strong reaction of monocytes/macrophages in vivo as opposed to the cited in vitro reports, and we also hypothesize that the severe formation of lens fi ber cells at 8 weeks on PMMA IOLs ( fi g. 4 ) occurred after the amelioration of monocyte/macrophage reaction, and resulted from these focal contacts.…”

Section: Lec Outgrowthsupporting

confidence: 57%

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Biological Compatibility of Polymethyl Methacrylate, Hydrophilic Acrylic and Hydrophobic Acrylic Intraocular Lenses

Barbour¹,

Saika²,

Miyamoto³

et al. 2005

Ophthalmic Res

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Purpose: Extensive clinical investigations of the biocompatibility of different intraocular lenses (IOLs) have been made in an effort to optimize the outcome of modern cataract surgery. The aim of this study was to add animal eye experimental implantation data regarding cellular reaction on the anterior surface of IOLs. Methods: Thirteen adult albino rabbits had phacoemulsification/aspiration of the crystalline lens followed by implantation of a posterior chamber IOL in each eye. Three types of IOLs were studied: Hydroview^® (Bausch & Lomb; n = 7), Acrysof^® (Alcon, USA; n = 7), and polymethyl methacrylate (PMMA; HOYA, Japan; n = 7). The animals were killed by intravenous pentobarbital 1, 4, or 8 weeks later. The IOLs were explanted and stained with hematoxylin and eosin, and observed under a light microscope. The shape of mouse ascites-induced macrophages on the anterior surface of the three different IOL types (Hydroview, PMMA, and Acrysof) was studied after 24 h of oven culture. Results: Hydrophilic acrylic IOLs showed the highest affinity for lens epithelial cell (LEC) outgrowth, and the lowest and slowest maturation rate reaction of macrophages. PMMA IOLs showed the lowest affinity for LEC outgrowth, and the highest reaction of macrophages. Hydrophobic acrylic IOLs showed intermediate results both regarding LECs and macrophages. Conclusions: Results suggest that IOL biomaterial properties are the key factor that influences the quantity of monocytes/macrophages as well as the process of their maturation/senescence. LEC outgrowth is influenced both by the biomaterial of IOLs and by the monocyte/macrophage reaction.

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Section: Lec Outgrowthsupporting

confidence: 57%

“…Regarding Hydroview IOLs, we propose that the later peak of mononuclear macrophages at 4 weeks ( fi g. 3 b) causes the regression of LECs ( fi g. 3 a) before resumption to outgrow thereafter. This observation of an inverse relation was also noticed from the impact of uveitic eyes [34,35] and increased BAB damage [3] on LEC outgrowth.…”

Section: Lec Outgrowthsupporting

confidence: 54%

Biological Compatibility of Polymethyl Methacrylate, Hydrophilic Acrylic and Hydrophobic Acrylic Intraocular Lenses

Barbour¹,

Saika²,

Miyamoto³

et al. 2005

Ophthalmic Res

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“…Together with clinical evidence indicating that PCO insurgence may be related to the type of IOL material [5,6,27], in vitro studies have shown that LECs preferentially adhere to PMMA rather than other IOL materials [15].…”

Section: Discussionmentioning

confidence: 93%

“…Biomaterials become coated with proteins soon after they are immersed in biological fluids and the cells grow and spread on their surface interacting with the proteins adsorbed on them [15,38,39].…”

Section: Discussionmentioning

confidence: 99%

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Bendazac Lysine Inhibition of Human Lens Epithelial Cell Adhesion to Polymethylmethacrylate Intraocular Lenses

Soldo¹,

Milanese²,

Pinza³

et al. 2004

Ophthalmic Res

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The aim of the present work was to evaluate the effect of bendazac lysine on the human lens epithelial cell line HLE-B3 adhesion to polymethylmethacrylate (PMMA) intraocular lenses (IOLs). After adherence to IOLs, cells were incubated in the presence of the drug for 24 h. The number of cells contained in a 6-mm² area was then counted with an inverted phase microscope and adherent cells were distinguished from detached floating cells by focusing through the medium. Results obtained show that bendazac is able to induce a linear dose-dependent inhibition of HLE-B3 adhesiveness to PMMA IOLs. In particular, treatment with bendazac 33, 100 and 300 µM resulted in a 15, 32 and 54% inhibition, respectively. Statistical analysis shows that this effect is significant at 100 µM (p < 0.05) and 300 µM (p < 0.01). The analysis of the effects of bendazac on the viability and on the proliferative capacity of HLE-B3 cells did not show any drug-related toxicity up to the concentration of 400 µM. The present study demonstrates that bendazac lysine is able to inhibit adhesion of lens epithelial cells to PMMA IOLs and suggests the potential beneficial use of this drug in preventing secondary cataract development.

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Stimulated Cellular Response of Novel Hybrid Network Structure Elastomers with Inorganic Short Chain Cross‐Links for Soft Tissue Reconstruction

et al. 2010

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The flexible and highly ductile silicone elastomer widely used as a soft‐tissue substitute for joint reconstruction and replacement fragments prematurely because of low tensile strength and inadequate bone build‐up around the implants. In this regard, an excellent vehicle to favorably modulate cellular response and retain intrinsic elastic recovery of soft tissue implants is to consider hybrid network structure elastomers with inorganic short chain cross‐links. The concept involves covalently linking nanocrystalline titania with a bi‐functional agent, acrylic acid, which has a carboxylic group to coordinate with titania and a vinyl group to form short chain cross‐links as an integral part of the silicone network during curing at elevated pressure. This approach provides high strength‐at‐break and undiminished intrinsic ductility of silicone elastomer and high cytocompatibility. Interestingly, cellular response is favorably modulated, as described here. The biologic response in terms of initial cell attachment, cell viability, and proliferation was consistently greater in relation to stand alone silicone elastomer such that cell spreading, morphology, and density were distinctly different. Pre‐osteoblasts grown on hybrid network structure elastomers were widely spread on the surface as a sheet after a culture time of 24 h. In contrast, these features were less pronounced on the stand alone silicone elastomer. Furthermore, immunofluorescence study illustrated distinct fibronectin expression level, stronger vinculin focal adhesion contacts associated with abundant actin stress fibers in pre‐osteoblasts grown on hybrid network structure elastomer compared to stand alone silicone elastomer, implying enhanced cell–substrate interactions. This finding was consistent with the observation of total protein content and sodium dodecyl sulfate polyacrylamide gel analysis. Based on the study described here, our hypothesis is that silicone–titania network structure elastomer with high strength–high ductility combination and favorable cellular response is a model hybrid network structure elastomer for joint reconstruction and soft tissue substitution. The integration of cellular and molecular biology with materials science and engineering aspects described here provides a new direction toward the development of new generation of hybrid network structure silicone elastomer. This subject is germane from the view point of fundamental understanding and from the view of hybrid elastomers as soft tissue reconstruction.

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Adhesion mechanisms of human lens epithelial cells on 4 intraocular lens materials

Abstract: This study confirms the multifactorial pathogenesis of posterior capsule opacification and suggests its incidence will be reduced by improving surgical techniques and using IOL surfaces that discourage cell adhesion.

Cited by 54 publications

References 26 publications

Biological Compatibility of Polymethyl Methacrylate, Hydrophilic Acrylic and Hydrophobic Acrylic Intraocular Lenses

Biological Compatibility of Polymethyl Methacrylate, Hydrophilic Acrylic and Hydrophobic Acrylic Intraocular Lenses

Bendazac Lysine Inhibition of Human Lens Epithelial Cell Adhesion to Polymethylmethacrylate Intraocular Lenses

Stimulated Cellular Response of Novel Hybrid Network Structure Elastomers with Inorganic Short Chain Cross‐Links for Soft Tissue Reconstruction

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