Adhesion mechanisms of lactic acid bacteria: conventional and novel approaches for testing

Alp, Duygu; Kuleaşan, Hakan

doi:10.1007/s11274-019-2730-x

Cited by 55 publications

(37 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…TA is the main component of the cell wall of Gram-positive bacteria. TA is composed of glycerol or ribitol chains connected by phosphoric acid and phosphodiester bridges [ 25 ]. Peptidoglycan is made up of mainly N-acetylmuramate and N-acetylglucosamine, which are derivatives of sugar, and several amino acids such as D-alanine, L-alanine, D-glutamic acid, and diaminopimelic acid.…”

Section: Resultsmentioning

confidence: 99%

“…A criterion for determining which LAB can be considered as probiotic is the bacteria’s capacity to attach itself to the epithelium of the intestinal mucosal [ 25 ]. Hydrophobicity is related to the presence of cell wall components, such as phospholipids, polysaccharides, and other external components on the bacterial cell surface [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

“…They have several important mechanisms for intestinal epithelial cell adhesion. They generally use various structures to adhere to intestinal cells, such as flagella, pili, S-layer proteins, lipoteichoic acid, EPSs, and mucus-binding proteins [ 25 ]. All the LAB in this research yielded negative results on the blood agar test, which involves hemolysis.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia

et al. 2020

View full text Add to dashboard Cite

Background and Aim: Probiotics play an important role in maintaining a healthy gut and consequently promote good health. This study aimed to find novel probiotic lactic acid bacteria (LAB) from indigenous fermented foods of West Sumatera, Indonesia. Materials and Methods: This study utilized 10 LAB previously isolated from fermented buffalo milk (dadih), fermented fish (budu), and fermented cassava (tape) which have the ability to produce gamma-aminobutyric acid. The study commenced with the screening of LAB for certain properties, such as resistance to acid and bile salts, adhesion to mucosal surface, and antagonism against enteric pathogens (Escherichia coli, Salmonella Enteritidis, and Staphylococcus aureus). The promising isolates were identified through biochemical and gram staining methods. Results: All isolates in this study were potential novel probiotics. They survived at a pH level of 2.5 for 3 h (55.27-98.18%) and 6 h (50.98-84.91%). Survival in bile at a concentration of 0.3% was 39.90-58.61% and the survival rate was 28.38- 52.11% at a concentration of 0.5%. The inhibitory diameter ranged from 8.75 to 11.54 mm for E. coli, 7.02 to 13.42 mm for S. aureus, and 12.49 to 19.00 mm for S. Enteritidis. All the isolates (84.5-92%) exhibited the ability to adhere to mucosal surfaces. This study revealed that all the isolates were potential probiotics but N16 proved to be superior because it was viable at a pH level of 2 (84.91%) and it had a good survival rate in bile salts assay (55.07%). This isolate was identified as Lactobacillus spp., Gram-positive bacilli bacteria, and tested negative in both the catalase and oxidase tests. Conclusion: All the isolates in this study may be used as probiotics, with isolate N16 (Lactobacillus spp.) as the most promising novel probiotic for poultry applications based on its ability to inhibit pathogenic bacteria.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia

et al. 2020

View full text Add to dashboard Cite

show abstract

“…To achieve a sustainable effect on the human microbiome, a strain must either be able to integrate stably into the microbiome or be supplied continuously. For stable integration, factors that favor inclusion into the gut microbiome must be considered, such as resistance to low pH, ability to tolerate bile salts, pancreatin, pepsin, lysozyme, and H 2 S [ 52 ], the ability to compete with other gut bacteria, and the ability to adhere to intestinal epithelial cells or mucus [ 59 , 60 ]. It may be possible to predict some of these factors by studying the genomes of the strains.…”

Section: Resultsmentioning

confidence: 99%

In Silico Comparison Shows that the Pan-Genome of a Dairy-Related Bacterial Culture Collection Covers Most Reactions Annotated to Human Microbiomes

et al. 2020

View full text Add to dashboard Cite

The diversity of the human microbiome is positively associated with human health. However, this diversity is endangered by Westernized dietary patterns that are characterized by a decreased nutrient variety. Diversity might potentially be improved by promoting dietary patterns rich in microbial strains. Various collections of bacterial cultures resulting from a century of dairy research are readily available worldwide, and could be exploited to contribute towards this end. We have conducted a functional in silico analysis of the metagenome of 24 strains, each representing one of the species in a bacterial culture collection composed of 626 sequenced strains, and compared the pathways potentially covered by this metagenome to the intestinal metagenome of four healthy, although overweight, humans. Remarkably, the pan-genome of the 24 strains covers 89% of the human gut microbiome’s annotated enzymatic reactions. Furthermore, the dairy microbial collection covers biological pathways, such as methylglyoxal degradation, sulfate reduction, γ-aminobutyric (GABA) acid degradation and salicylate degradation, which are differently covered among the four subjects and are involved in a range of cardiometabolic, intestinal, and neurological disorders. We conclude that microbial culture collections derived from dairy research have the genomic potential to complement and restore functional redundancy in human microbiomes.

show abstract

“…This finding implied that these three bifidobacteria strains could effectively adhere to intestinal epithelial cells. The components of bifidobacteria act as adhesive factors, including lipoteichoic acids, carbohydrates, extracellular proteins, and surface proteins located on the outer surface of the cell wall ( Fanning et al, 2012a ; González-Rodríguez et al, 2012 ; Alp and Kuleaşan, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Potential Immunomodulatory Activity of a Selected Strain Bifidobacterium bifidum H3-R2 as Evidenced in vitro and in Immunosuppressed Mice

et al. 2020

View full text Add to dashboard Cite

The microbiota is directly involved in the development and modulation of the intestinal immune system. In particular, members of the genus Bifidobacterium play a primary role in immune regulation. In the present study, Bifidobacterium bifidum H3-R2 was screened from 15 bifidobacterium strains by in vitro experiment, showing a positive tolerance to digestive tract conditions, adhesion ability to intestinal epithelial cells and a regulatory effect on immune cell activity. Immunostimulatory activity of B. bifidum H3-R2 was also elucidated in vivo in cytoxan (CTX)-treated mice. The results showed that the administration of B. bifidum H3-R2 ameliorated the CTX-induced bodyweight loss and imbalanced expression of inflammatory cytokines, enhanced the production of secretory immunoglobulin A (SIgA), and promoted splenic lymphocyte proliferation, natural killer (NK) cell activity and phagocytosis of macrophages in immunosuppressed mice. In addition, B. bifidum H3-R2 restored injured intestinal mucosal, and increased the villus length and crypt depth in CTX-treated mice. The results could be helpful for understanding the functions of B. bifidum H3-R2, supporting its potential as a novel probiotic for immunoregulation.

show abstract

Adhesion mechanisms of lactic acid bacteria: conventional and novel approaches for testing

Cited by 55 publications

References 78 publications

Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia

Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia

In Silico Comparison Shows that the Pan-Genome of a Dairy-Related Bacterial Culture Collection Covers Most Reactions Annotated to Human Microbiomes

Potential Immunomodulatory Activity of a Selected Strain Bifidobacterium bifidum H3-R2 as Evidenced in vitro and in Immunosuppressed Mice

Contact Info

Product

Resources

About