Adhesion of corneal epithelial cells to cell adhesion peptide modified pHEMA surfaces

Merrett, Kimberley; Griffith, May; Deslandes, Yves; Pleizier, G.; Sheardown, Heather

doi:10.1163/156856201316883467

Cited by 71 publications

(49 citation statements)

References 46 publications

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“…Surprisingly, the adsorption of laminin to the surfaces prior to culture, while resulting in an increase in the epithelial cell coverage relative to the controls, did not provide the dramatic effect expected based on our previous results. 14,15 The effect of the TGF-␤2 and its activity on the surface is clearly demonstrated by the increases in epithelial cell coverage on all surfaces with TGF-2 (relative to the aminated controls) and more importantly by the significant decrease in coverage in the presence of anti-TGF-␤ under serum-free conditions.…”

Section: Cell Culture Studiesmentioning

confidence: 99%

“…Stromal cells were cultured in either Dubellco's Modified Eagle Medium (DMEM) (Gibco BRL Life Technologies, Burlington, ON) supplemented with insulin-transferrin-selenium (1 mL/L) (ITS) and gentamycin or DMEM supplemented with ITS, gentamycin, and 10% fetal bovine serum (FBS). Additional surfaces were preadsorbed with laminin based on positive results with YIGSR and related peptides in our previous work 14,15 for a period of 2 h prior to culture under serum-free conditions. Samples were examined on a daily basis for cell attachment, spread, and proliferation.…”

Section: Cell Culture Studiesmentioning

confidence: 99%

“…Adsorption of serum proteins or specific adsorption of collagen or fibronectin has been used to promote the migration of stromal keratocytes into the pores of a polymeric support and the subsequent production of collagen. 4 -6 Techniques to promote epithelialization have included surface modification with cell adhesion proteins, [7][8][9][10][11][12] either by passive adsorption or covalent attachment, surface modification, with cell adhesion peptides 7,[13][14][15] or plasma modification of the optic surface. 16 -20 Although significant progress has been made in the development of surfaces that promote the adhesion of both cell types, the problem of epithelial downgrowth at the interface between the prosthesis and the native tissue has not been adequately addressed, and remains a significant problem to be overcome.…”

Section: Introductionmentioning

confidence: 99%

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Interactions of corneal cells with transforming growth factor β2‐modified poly dimethyl siloxane surfaces

Merrett

Griffith

Deslandes

et al. 2003

J Biomedical Materials Res

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The downgrowth of corneal epithelial cells at the interface of an artificial cornea and the host eye tissue poses a significant problem to be overcome in developing a successful implant. As a means of inhibiting the proliferation of corneal epithelial cells on the stromal surface of the implant, we examined the immobilization of transforming growth factor beta-2 (TGF-beta2) via a bifunctional poly ethylene glycol (PEG) spacer to poly dimethyl siloxane (PDMS) surfaces. Growth factor immobilization was confirmed by modification with (125)I-labeled TGF-beta 2. The modified surfaces were also characterized by advancing water contact angles, X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). Although the amount of growth factor covalently bound to the surface was difficult to quantify apparently due to strong interactions between the growth factor and the PEG layer and high levels of adsorption, differences in the modified surfaces, suggestive of the presence of a significant amount of TGF-beta 2, were found. In vitro interactions of the modified surfaces with human corneal epithelial and stromal cells were examined. Growth factor surface concentrations as well as culture in the absence and presence of serum and other adhesive proteins were examined. Corneal stromal and epithelial cells cultured on the TGF-beta 2-modified surfaces consistently gave results opposite to those expected. Likely, the most notable and surprising result was the almost complete lack of adhesion of the stromal cells, with coverage averaging between 3 and 5%. In comparison, corneal epithelial cell growth appeared to be promoted by the presence of the immobilized growth factor, with cell coverage averaging 50-60% at 7 days of culture. A TGF-beta 2 concentration effect was noted with both cell types in the absence of serum, with increases in the coverage at higher TGF-beta 2 concentrations. The observed cell growth appeared to be the result of interactions between the cells and active growth factor, because the addition of anti-TGF-beta 2 to the culture medium reduced cell coverage to levels similar to those noted on control surfaces. Therefore, although TGF-beta 2-modified surfaces may not be suitable as corneal epithelial cell inhibiting surfaces, interactions of surface immobilized growth factor and corneal cells are complex and should be further examined.

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Section: Cell Culture Studiesmentioning

confidence: 99%

Section: Cell Culture Studiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Interactions of corneal cells with transforming growth factor β2‐modified poly dimethyl siloxane surfaces

Merrett

Griffith

Deslandes

et al. 2003

J Biomedical Materials Res

Self Cite

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show abstract

“…Although hydrogels characteristically have low protein adsorption and therefore low native cell interactions, many studies have examined addition of biological molecules to promote cell interactions. These include addition of adhesion peptides such as RGD and YIGSR [142][143][144][145][146] which enhance interactions of specific cell types, polysaccharides such as heparin that are able to present growth factors [147,148], and growth factors themselves [149,150].…”

Section: Biological Evaluationmentioning

confidence: 99%

Conducting polymer-hydrogels for medical electrode applications

Green

Baek

Poole-Warren

et al. 2010

Science and Technology of Advanced Materials

247

182

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Conducting polymers hold significant promise as electrode coatings; however, they are characterized by inherently poor mechanical properties. Blending or producing layered conducting polymers with other polymer forms, such as hydrogels, has been proposed as an approach to improving these properties. There are many challenges to producing hybrid polymers incorporating conducting polymers and hydrogels, including the fabrication of structures based on two such dissimilar materials and evaluation of the properties of the resulting structures. Although both fabrication and evaluation of structure-property relationships remain challenges, materials comprised of conducting polymers and hydrogels are promising for the next generation of bioactive electrode coatings.

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“…Cell adhesion peptides, including RGD, YIGSR, and a novel collagen peptoid Gly-Pro-nLeu, have also been studied, but these peptide/peptoid modifications have produced only modest increases in the rate of epithelialization. [37][38][39][40][41] To date, although a few replacement materials with either coating and/or direct attachment of biological factors (both proteins and peptides) to their surfaces have had some short-term success in maintaining a somewhat normal epithelial layer, long-term analysis (up to 8 years) has shown that the density of the epithelial layer is not maintained. [42][43][44][45] We have investigated the chemical tethering of extracellular matrix proteins and peptides with epithelial cell-surface adhesion binding activities to a hydrogel surface.…”

Section: Introductionmentioning

confidence: 99%

Corneal epithelial cell growth over tethered‐protein/peptide surface‐modified hydrogels

Jacob

Rochefort

et al. 2004

J Biomed Mater Res

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In this study, we investigated the corneal epithelial cell growth rate and adhesion to novel hydrogels with (1) extracellular matrix proteins [fibronectin, laminin, substance P, and insulin-like growth factor-1 (IGF-1)] and (2) peptide sequences [RGD and fibronectin adhesion-promoting peptide (FAP)] tethered to their surface on poly(ethylene glycol) (PEG) chains. The growth rate to confluence of primary rabbit cornea epithelial cells was compared for plain polymethacrylic acid-co-hydroxyethyl methacrylate (PHEMA/MAA) hydrogels, PHEMA/MAA hydrogels coated with extracellular matrix proteins or peptides, and PHEMA/ MAA hydrogels with tethered extracellular matrix proteins or peptides on the surface. The development of focal adhesions by the epithelial cells grown on the surfaces was determined by F-actin staining. Little to no epithelial cell growth occurred on the plain hydrogel surfaces throughout the 15-day culture period. Of the coated hydrogels, only the fibronectin-coated surfaces showed a significant increase in cell growth compared to plain hydrogels (p < 0.009). However, even these surfaces reached a maximum of only 20% confluence. Laminin, fibronectin adhesion-promoting peptide (FAP), and fibronectin/laminin (1:1) tether-modified hydrogels all achieved 100% confluence by the end of the culture period, although the rates at which confluence was reached differed. F-actin staining showed that focal adhesions were formed for the laminin, FAP, and fibronectin/laminin tether-modified surfaces. The results support the hypothesis that tethering certain extracellular matrix proteins and/or peptides to the hydrogel surface enhances epithelial cell growth and adhesion, compared with that seen for proteincoated or plain hydrogel surfaces.

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Adhesion of corneal epithelial cells to cell adhesion peptide modified pHEMA surfaces

Cited by 71 publications

References 46 publications

Interactions of corneal cells with transforming growth factor β2‐modified poly dimethyl siloxane surfaces

Interactions of corneal cells with transforming growth factor β2‐modified poly dimethyl siloxane surfaces

Conducting polymer-hydrogels for medical electrode applications

Corneal epithelial cell growth over tethered‐protein/peptide surface‐modified hydrogels

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