Adhesion of Corynebacterium renale, Corynebacterium pilosum, and Corynebacterium cystitidis to bovine urinary bladder epithelial cells of various ages and levels of differentiation

Abstract

The adhesion of Corynebacterium renale, Corynebacterium pilosum, and Corynebacterium cystitidis to various epithelial cell layers of bovine urinary bladders was examined. Adhesion was most efficient to the urinary sediment epithelial cells and the superficial cells immediately before shedding, followed by the remaining superficial cells and intermediate cells in this order, and least efficient to the deeper intermediate and basal cells. Incubation of the intermediate cells for 6 h increased the number of bacteria that adhered to these cells.

Isolation of Corynebacterium pilosum and Actinomyces pyogenes from Cystitis and Vulvovaginitis Infection in a 2‐month‐old Female C

“…The adhesion of corynebacteria to urinary bladder epithelial cells is thought to be a prerequisite for the development of bovine cystitis and pyelonephritis (GILLESPIE and TIMONEY, 1981). Adhesion of C. renale, C. pilosum and C. cystitidis to the epithelial cells from bovine urinary bladders has been demonstrated (TAKAI et al, 1980;SATO et al, 1982). Although C. pilosum was not isolated from the urinary bladder epithelium, it probably played an important role, together with Actinomyces pyogenes, in the pathogenesis of the described disease.…”

Clinical presentations and antimicrobial susceptibilities of Corynebacterium cystitidis associated with renal disease in four beef cattle

“…Of the species C. cystitidis , C. renale , and C. pilosum , C. renale is thought to be the most virulent pathogen 3 . Corynebacterium cystitidis , C. renale , and C. pilosum preferentially adhere to bladder epithelial cells in contact with the urine and older superficial cells (immediately before shedding), indicating that adhesion probably occurs in senescent cells first 4 …”

Genomic differences among strains of Corynebacterium cystitidis isolated from uterus of camels

“…Recently, Smith et al, 2020 reported the isolation of C. cystitidis from 4 cases of pyelonephritis in cow [3]. Currently, studies on C. cystitidis are rare with one study showing bacterium ability to survive in soil for two months [4] and another study describing adhesion to urinary epithelial cells through pili structures existing on the bacteria cell surface [5]. Yet, there is no information about uterine infection caused by C. cystitidis in animals nor report of isolation from others site in animals than cow.…”