Adhesion of endometrial cells labeled with 111Indium-tropolonate to peritoneum: a novel in vitro model to study endometriosis

Abstract

Objective: To evaluate, in a new original in vitro assay, putative factors that could modulate the adhesion of endometrial cells to peritoneum. Design: Prospective, controlled in vitro study. Setting: Academic research laboratory. Result(s):The adhesion of human endometrial cells to mouse peritoneum was increased by treatment with proinflammatory cytokines (interleukin IL-1 β, IL-6, TNF α, TGF-β1). Whereas heparan sulfate had no effect on cell adhesion, a gel of ferric hyaluronate (Intergel) was able to counteract the pro-adhesive effect of cytokines. Interestingly, the pretreatment of peritoneum with cytokines, 24 hours before cell seeding in the presence of the ferric hyaluronate gel, restored the cytokine-promoting effect on cell adhesion. Conclusion(s):Proinflammatory cytokines promote the in vitro peritoneal adhesion of endometrial cells. An antiadhesive hyaluronate gel used in clinics decreases the adhesion in a dose-dependent manner and reduces cytokine bioavailability.

Association of body mass index with severity of endometriosis in Korean women

“…Current investigation being chiefly focused on the metabolism and biological action of adipokines, only a few studies on the relation between endometriosis and adipokines have been published to date. Leptin, an angiogenic adipokine, appears to have a favorable effect on endometrial lesions [17]-while tumor necrosis factor α and some interleukins appear to have a favorable effect on endometrial adhesions in the peritoneal cavity [18,19]. In addition, adiponectin was recently found to have an anti-inflammatory and anti-angiogenic effect [20].…”

Endometriosis and Ovarian Cancer

“…These factors promote the adhesion of endometrial cells to peritoneum in patients with endometriosis (86). …”

Increased activation of nuclear factor-kappa B (NF-κB) in isolated peritoneal macrophages of patients with endometriosis

“…Indeed, upon arrival in the peritoneal cavity, endometrial tissue is likely to adhere to the mesothelial lining (14). This adhesion process may be promoted by cell adhesion molecules and soluble factors produced by peritoneal macrophages (15). Endometrial tissue may also adhere if there is too much regurgitated endometrial tissue or if the capacity of intra-abdominal cells to clear the abdominal cavity is impaired (4,5).…”