Adhesion of fibroblasts on micro- and nanostructured surfaces prepared by chemical vapor deposition and pulsed laser treatment

Abstract

The development of micro- and nanostructured surfaces which improve the cell-substrate interaction is of great interest in today's implant applications. In this regard, Al/Al2O3 bi-phasic nanowires were synthesized by chemical vapor deposition of the molecular precursor (tBuOAlH2)2. Heat treatment of such bi-phasic nanowires with short laser pulses leads to micro- and nanostructured Al2O3 surfaces. Such surfaces were characterized by scanning electron microscopy (SEM), electron dispersive spectroscopy and x-ray photoelectron spectroscopy. Following the detailed material characterization, the prepared surfaces were tested for their cell compatibility using normal human dermal fibroblasts. While the cells cultivated on Al/Al2O3 bi-phasic nanowires showed an unusual morphology, cells cultivated on nanowires treated with one and two laser pulses exhibited morphologies similar to those observed on the control substrate. The highest cell density was observed on surfaces treated with one laser pulse. The interaction of the cells with the nano- and microstructures was investigated by SEM analysis in detail. Laser treatment of Al/Al2O3 bi-phasic nanowires is a fast and easy method to fabricate nano- and microstructured Al2O3-surfaces for studying cell-surface interactions. It is our goal to develop a biocompatible Al2O3-surface which could be used as a coating material for medical implants exhibiting a cell selective response because of its specific physical landscape and especially because it promotes the adhesion of osteoblasts while minimizing the adhesion of fibroblasts.

Reduced myofibroblast differentiation on femtosecond laser treated 316LS stainless steel

“…Dalby et al recently described filopodia as possible sensors for the three-dimensional environment [28]. Previously, we postulated that an enhanced production of such filopodia by fibroblasts is characteristic for a better cell adherence on a certain surface [29]. In the present context, the phenomenon of increased filopodia appears only on non-treated steel surfaces and coincides with the highest cell numbers and the highest MF differentiation, thus supporting previous results.…”

Guidance of glial cells and neurites from dorsal root ganglia by laser induced periodic patterning of biphasic core/shell nanowires

“…Two‐beam LIP provided linear periodic patterns on the biphasic Al/Al 2 O 3 layer and energy dispersive X‐ray spectroscopy (EDX) analysis shows only the presence of Al and O peaks (not shown here). Furthermore our previous investigation showed that as‐deposited and laser treated Al/Al 2 O 3 NWs exhibit identical chemistry . The core‐shell biphasic NWs act as a self‐healing material.…”

Recombinant Phage Coated 1D Al2O3Nanostructures for Controlling the Adhesion and Proliferation of Endothelial Cells

“…The decrease in cell viabilities and populations can be explained by the difference in the surface topography induced by the deposited 1D Al 2 O 3 nanostructures [ 18 , 19 ]. However, overcoating of 1D Al 2 O 3 nanostructures with re-phages led to a clear increase in cell populations and viabilities around 40% and 30%, respectively, as compared to 1D Al 2 O 3 nanostructures.…”