Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4

Crosby, Heather A.; Ross, Emma J.; Newsome, Philip N.; Adams, David H.

doi:10.1016/j.jhep.2009.06.021

Cited by 38 publications

(32 citation statements)

References 45 publications

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“…Likewise, it has been shown that SDF-1 may increase, in vivo and in vitro, the adhesion of inflammatory cells to the endothelium [27,39]. These results can be explained by the capacity of SDF-1 to upregulate the expression of various adhesion …”

Section: Sdf-1 and Cxcr4 Expression By Lsecmentioning

confidence: 93%

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Stromal-Derived Factor-1 and Its Receptor, CXCR4, Are Constitutively Expressed by Mouse Liver Sinusoidal Endothelial Cells: Implications for the Regulation of Hematopoietic Cell Migration to the Liver During Extramedullary Hematopoiesis

Mendt

Cardier

2012

Stem Cells and Development

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Stromal-derived factor (SDF)-1 is the main regulating factor for trafficking/homing of hematopoietic stem cells (HSC) to the bone marrow (BM). It is possible that this chemokine may also play a fundamental role in regulating the migration of HSC to several organs during extramedullary hematopoiesis. Because liver sinusoidal endothelial cells (LSEC) constitute an extramedullary niche for HSC, it is possible that these cells represent one of the main cellular sources of SDF-1 at the liver. Here, we show that LSEC express SDF-1 at the mRNA and protein level. Biological assays showed that conditioned medium from LSEC (LSEC-CM) stimulated the migration of BM progenitor lineage-negative (BM/Lin -) cells. This effect was significantly reduced by AMD3100, indicating that the SDF-1/CXCR4 axis is involved in the stimulatory migrating effect induced by LSEC-CM. Early localization of HSC in SDF-1-expressing LSEC microenvironment together with increased levels of this chemokine in hepatic homogenates was found in an experimental model of liver extramedullary hematopoiesis. Flow cytometry studies showed that LSEC express the CXCR4 receptor. Functional assays showed that activation of this receptor by SDF-1 stimulated the migration of LSEC and increased the expression of PECAM-1. Our findings suggest that LSEC through the production of SDF-1 may constitute a fundamental niche for regulation of HSC migration to the liver. To our knowledge, this is the first report showing that LSEC not only express and secrete SDF-1, but also its receptor CXCR4.

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Section: Sdf-1 and Cxcr4 Expression By Lsecmentioning

confidence: 93%

“…These findings suggest that LSEC may provide signals to regulate the migration and homing of hematopoietic cells to the liver, during extramedullary hematopoiesis [20,27]. In vitro, we have shown that cloned LSEC support the multilineage differentiation of murine hematopoietic cells HSC [19,28].…”

Section: Introductionmentioning

confidence: 99%

Stromal-Derived Factor-1 and Its Receptor, CXCR4, Are Constitutively Expressed by Mouse Liver Sinusoidal Endothelial Cells: Implications for the Regulation of Hematopoietic Cell Migration to the Liver During Extramedullary Hematopoiesis

Mendt

Cardier

2012

Stem Cells and Development

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“…Interactions between this ECM glycosaminoglycan and CD44 are known to facilitate migration of leukocytes to inflamed tissue, as well as the progression of inflammatory injury [67]. Alternatively, CD44 has been implicated in the resolution of injury by facilitating the migration of hematopoietic stem cells to the injured liver [68]. CD44 interactions with HA are therefore an interesting point for further investigation.…”

Section: The Ecmmentioning

confidence: 99%

Transitional Remodeling of the Hepatic Extracellular Matrix in Alcohol-Induced Liver Injury

Poole

Arteel

2016

BioMed Research International

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Alcohol consumption is a common custom worldwide, and the toxic effects of alcohol on several target organs are well understood. The liver is the primary site of alcohol metabolism and is therefore the major target of alcohol toxicity. Alcoholic liver disease is a spectrum of disease states, ranging from simple steatosis (fat accumulation), to inflammation, and eventually to fibrosis and cirrhosis if untreated. The fibrotic stage of ALD is primarily characterized by robust accumulation of extracellular matrix (ECM) proteins (collagens) which ultimately impairs the function of the organ. The role of the ECM in early stages of ALD is poorly understood, but recent research has demonstrated that a number of changes in the hepatic ECM in prefibrotic ALD not only are present, but may also contribute to disease progression. The purpose of this review is to summarize the established and proposed changes to the hepatic extracellular matrix (ECM) that may contribute to earlier stages of ALD development and to discuss potential mechanisms by which these changes may mediate the progression of the disease.

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“…The CD49e molecule, also known as integrin α5, mediates cell adhesion and participates in cell-surface mediated signalling [27]. CD44 (H-CAM), a cell membrane glycoprotein involved in cell-cell interactions, cell adhesion and migration, is expressed on stem cell populations isolated from adipose tissue [24], bone marrow [28], astrocyte precursor cells [29], and hemopoietic stem cells [30]. The CD13 protein, a zincmetallopeptidase that cleaves single neutral amino acids, is expressed not only on the surface of early committed granulocyte and monocyte progenitors and endothelial cells, but also on BM stromal cells [31].…”

Section: Mscs From the Subendothelial Layermentioning

confidence: 99%

Phenotype and Differentiation Potential of Stromal Populations Obtained from Various Zones of Human Umbilical Cord: An Overview

Conconi

Liddo²,

Tommasini³

et al. 2011

TOTERMJ

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Fibroblast-like cells with properties similar to mesenchymal stromal cells (MSCs) are present in human umbilical cord (hUC). In accordance with the international minimal criteria for defining multipotent mesenchymal stromal cells, hUC cells are designed MSCs being plastic adherent, positive to specific non hematopoietic lineage biomarkers, able to be both in vitro long term cultured and differentiated into osteoblasts, chondroblasts and adipocytes. In this review, a panoramic view of phenotypic characteristics of hUC cells derived from various UC parts are described. The high heterogeneity of extraction, culture and analysis procedures hinder the ability to precisely identify UC stromal cells. As a result, different phenotypic profiles are detectable not only among the cells obtained from the various parts of cord, but also inside the same UC regions, suggesting that UC-MSCs may represent an unique cell family whose components present various degree of stemness. However, in vitro and in vivo evidence indicates Wharton's jelly as the best source of MSCs, because its cells present a wide range of potential therapeutic applications.

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Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4

Cited by 38 publications

References 45 publications

Stromal-Derived Factor-1 and Its Receptor, CXCR4, Are Constitutively Expressed by Mouse Liver Sinusoidal Endothelial Cells: Implications for the Regulation of Hematopoietic Cell Migration to the Liver During Extramedullary Hematopoiesis

Stromal-Derived Factor-1 and Its Receptor, CXCR4, Are Constitutively Expressed by Mouse Liver Sinusoidal Endothelial Cells: Implications for the Regulation of Hematopoietic Cell Migration to the Liver During Extramedullary Hematopoiesis

Transitional Remodeling of the Hepatic Extracellular Matrix in Alcohol-Induced Liver Injury

Phenotype and Differentiation Potential of Stromal Populations Obtained from Various Zones of Human Umbilical Cord: An Overview

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