Adhesion of<i>Helicobacter pylori</i>to Human Gastric Mucosal Biopsy Specimens Cultivated in Vitro

Rosberg, K.; Berglindh, T.; Gustavsson, Sven; R, Hübinette; Rolfsen, W

doi:10.3109/00365529108998611

Cited by 15 publications

(12 citation statements)

References 22 publications

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“…The total preparation time was approximately 2 h and 30min. After 6 h in culture, at which point the mucosae had adhered to the grid, specimens were inoculated with H. pylori or H. mustelae (35,36). The culture medium used was a modified Autrup's medium in which penicillin and streptomycin were replaced by nalidixic acid, 20 pg/ml; amphotericin (Fungizone@), 0.25 pg/ml; and Skirrow's antibiotic supplement, 0.4 mVl00 ml medium (Oxoid, Ltd., cat.…”

Section: Pig Mucosamentioning

confidence: 99%

“…In addition, strain 69Am, a genetically manipulated mutant lacking flagella, and its parent strain, 69A (kindly provided by Dr. Haas, Max-Planck-Institut, Tubingen, Germany), were tested in pig antral specimens. All strains were cultured on blood agar plates in a microaerophilic atmosphere during 48 h before inoculation (35,36). The strains were passed a maximum of 10 times before inoculation.…”

Section: Strainsmentioning

confidence: 99%

“…Antral mucosa from 28 pigs, urinary bladder from 6 pigs, and duodenal mucosa from 6 pigs were included in this study. Mucosal biopsy specimens were prepared as previously described (35,36). In brief, the mucosa was separated from the muscle layer, and 2-mm-wide specimens were punched out.…”

Section: Pig Mucosamentioning

confidence: 99%

“…Our previous reports on the gastric mucosa biopsy culture model for studies of H. pylori adhesion to pig and human biopsy specimens showed an increased adhesion over a much longer period of timethat is, 72 h (35,36). The pattern of H. pylori adhesion to pig antral specimens closely resembled that of human gastric mucosa (36), and electron micrographs show an intimate contact between H. pylori and microvilli/epithelial cells (37). Further, since the mucosal specimen is intact, with a functioning mucus production, we believe that this model more closely resembles the in vivo conditions than other in vitro models and would thus be more suitable for studies of H. pylori adhesion.…”

mentioning

confidence: 95%

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HelicobacterColonization of Biopsy Specimens Cultured in Vitro Is Dependent on Both Mucosal Type and Bacterial Strain

Meyer-Rosberg

Berglindh

1996

Scandinavian Journal of Gastroenterology

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Section: Pig Mucosamentioning

confidence: 99%

Section: Strainsmentioning

confidence: 99%

Section: Pig Mucosamentioning

confidence: 99%

mentioning

confidence: 95%

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HelicobacterColonization of Biopsy Specimens Cultured in Vitro Is Dependent on Both Mucosal Type and Bacterial Strain

Meyer-Rosberg

Berglindh

1996

Scandinavian Journal of Gastroenterology

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“…8] or gastric adenocarcinoma cells [10]. However, when primary gastric cells were used [11][12][13] no association with putative adherence factors was made.…”

Section: Introductionmentioning

confidence: 99%

Loss of <i>Helicobacter pylori </i>Hemagglutination with Serial Laboratory Passage and Correlation of Hemagglutination with Gastric Epithelial Cell Adherence

et al. 1996

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Adherence of Helicobacter pylori to gastric epithelial cells is thought to be important in the pathogenesis of infection and may be essential to maintain lifelong colonization. However, the factors responsible for adherence to gastric epithelial cells in vivo have not been characterized, and the significance of adherence to standard epithelial cell lines is unclear. Hemagglutination is also thought to be important in H pylori adherence. However, no studies have clearly linked H. pylori hemagglutination or adherence to cultured epithelial cells to primary gastric epithelial cell adherence. Furthermore, it is not clear whether laboratory strains which have undergone multiple passages lose potential colonization factors. In this study, we examined the effect of serial laboratory passage on hemagglutination and correlated the hemagglutination characteristics of H. pylori strains to primary gastric cell adherence. Variable expression of hemagglutination was seen with serial laboratory passage of 15 strains. After 100 serial laboratory passages, all strains had lost hemagglutination activity. Hemagglutination was seen in association with adherence to primary gastric cells in vitro isolated from 2 patients. An association with ultrastructural intimate adherence was seen with HEp-2 cells, but not with gastric adenocarcinoma cells. Ultrastructural adherence was seen in corresponding antral biopsies of patients whose strains were hemagglutination positive, but hemagglutination was not associated with gastric inflammation. These data indicate that H. pylori hemagglutination is lost with serial passage and that hemagglutination may play a role in the attachment of H. pylori to gastric epithelial cells, but the role of adherence to chronic gastric inflammation is unclear.

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Recent Developments in Bacterial Adhesion to Animal Cells

Ofek¹,

Doyle²

1994

Bacterial Adhesion to Cells and Tissues

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Adhesion ofHelicobacter pylorito Human Gastric Mucosal Biopsy Specimens Cultivated in Vitro

Cited by 15 publications

References 22 publications

HelicobacterColonization of Biopsy Specimens Cultured in Vitro Is Dependent on Both Mucosal Type and Bacterial Strain

HelicobacterColonization of Biopsy Specimens Cultured in Vitro Is Dependent on Both Mucosal Type and Bacterial Strain

Loss of <i>Helicobacter pylori </i>Hemagglutination with Serial Laboratory Passage and Correlation of Hemagglutination with Gastric Epithelial Cell Adherence

Recent Developments in Bacterial Adhesion to Animal Cells

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