Adhesion of <i>Listeria monocytogenes</i> to materials commonly found in domestic kitchens

Teixeira, P.; Lima, Joana Madureira; Azeredo, Joana; Oliveira, Rosário

doi:10.1111/j.1365-2621.2007.01598.x

Cited by 24 publications

(19 citation statements)

References 27 publications

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“…20,29 Glass also has physicochemical surface properties similar to those of stainless steel: The free energies of adhesion (derived from surface-free energies from total, Lifshitz-van der Waals interactions and hydrogen bonds) of L. monocytogenes cells onto glass and stainless steel are almost identical 29 ; both materials are hydrophobic. 30 A microscopic glass slide (Fisher Scientific, Fair Lawn, NJ, USA) was cut into small pieces (22×22 mm). The piece was cleaned by immersing in 70% (v/v) nitric acid for 1 h, washed and rinsed with distilled water, sterilized with 70% (v/v) ethanol, and then dried for 10 min prior to use.…”

Section: Preparation Of Test Glass Surfaces and Bacterial Culturesmentioning

confidence: 99%

Positive Correlation Between the Expression of inlA and inlB Genes of Listeria monocytogenes and Its Attachment Strength on Glass Surface

et al. 2009

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Previous research involving internalin A (inlA) and internalin B (inlB) single-and double-knockout mutants of Listeria monocytogenes has suggested the involvement of two surface proteins, InlA and InlB, in the adherence of the cells to a glass surface. This phenomenon was further investigated with a larger number (n=27) of L. monocytogenes wild-type strains that were isolated from catfish processing plants and catfish fillets, in addition to internal controls, one ATCC 7644 strain, and L. monocytogenes EGDe strain. Of the wild-type strains, three were shown to produce truncated forms of InlA protein. A blot succession method was used to measure the ease of detachment of sessile L. monocytogenes from a glass surface after attachment at 4°C for 8 h. Real-time reverse transcriptase polymerase chain reaction was used to quantitate mRNA levels of inlA and inlB in L. monocytogenes strains after the cells were incubated at 4°C for 8 h. An inverse relationship between the ease of cell removal from glass surface and the relative inlA and inlB mRNA levels with R 2 value of 0.664 and 0.431, respectively, was observed. This suggests that the attachment strength of L. monocytogenes on glass surface is positively correlated with inlA and inlB expression. There were no differences (p>0.05) in attachment strength among serotypes. These results suggest that L. monocytogenes InlA and InlB proteins play a role in adherence to a glass surface at low temperature, and the attachment ability is independent of serotype.

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Section: Preparation Of Test Glass Surfaces and Bacterial Culturesmentioning

confidence: 99%

Positive Correlation Between the Expression of inlA and inlB Genes of Listeria monocytogenes and Its Attachment Strength on Glass Surface

et al. 2009

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“…Therefore, it is believed that the physical relief of surfaces favors bacterial adhesion, as do surface free energies. Stainless steel surfaces are thus prone to the formation of biofilms because of their great surface free energy, from which their hydrophilic characteristics derive [78]. In our study, it was found that both stainless steel and polyester surfaces without biocide were suitable for the adhesion and formation of biofilms, considering that polyester surfaces are much more porous and suitable for fixing microorganisms.…”

Section: Bacterial Count By Culture and Evaluation Of The Biofilm Formentioning

confidence: 58%

Antimicrobial Activity and Prevention of Bacterial Biofilm Formation of Silver and Zinc Oxide Nanoparticle-Containing Polyester Surfaces at Various Concentrations for Use

Fontecha‐Umaña

Ríos-Castillo

Ripolles‐Avila

et al. 2020

Foods

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Food contact surfaces are primary sources of bacterial contamination in food industry processes. With the objective of preventing bacterial adhesion and biofilm formation on surfaces, this study evaluated the antimicrobial activity of silver (Ag-NPs) and zinc oxide (ZnO-NPs) nanoparticle-containing polyester surfaces (concentration range from 400 ppm to 850 ppm) using two kinds of bacteria, Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli), and the prevention of bacterial biofilm formation using the pathogen Listeria monocytogenes. The results of antimicrobial efficacy (reductions ≥ 2 log CFU/cm 2 ) showed that at a concentration of 850 ppm, ZnO-NPs were effective against only E. coli (2.07 log CFU/cm 2 ). However, a concentration of 400 ppm of Ag-NPs was effective against E. coli (4.90 log CFU/cm 2 ) and S. aureus (3.84 log CFU/cm 2 ). Furthermore, a combined concentration of 850 ppm Ag-NPs and 400 ppm ZnO-NPs showed high antimicrobial efficacy against E. coli (5.80 log CFU/cm 2 ) and S. aureus (4.11 log CFU/cm 2 ). The results also showed a high correlation between concentration levels and the bacterial activity of Ag-ZnO-NPs (R 2 = 0.97 for S. aureus, and R 2 = 0.99 for E. coli). They also showed that unlike individual action, the joint action of Ag-NPs and ZnO-NPs has high antimicrobial efficacy for both types of microorganisms. Moreover, Ag-NPs prevent the biofilm formation of L. monocytogenes in humid conditions of growth at concentrations of 500 ppm. Additional studies under different conditions are needed to test the durability of nanoparticle containing polyester surfaces with antimicrobial properties to optimize their use.Foods 2020, 9, 442 2 of 15 studies include the development of antimicrobial surfaces capable of preventing and/or inhibiting bacterial adhesion [8][9][10][11].Metal agents such as silver, copper, zinc, titanium, and cobalt have antimicrobial properties. When these agents remain on surfaces, their biocidal properties are gradually released onto the surface through a process of ion exchange, providing continuous, lasting antimicrobial activity [12][13][14]. The biocidal properties of metal agents depend on the characteristics of their nanoparticles, such as size, distribution, morphology, and the release of very small quantities of their cations on the substrates on which they are acting [15,16]. The antimicrobial activity of these agents is also affected by extrinsic factors, such as the pH of the medium, time of exposure, concentration, UV radiation, and the presence of other substances or microorganisms [17][18][19].Two of the most extended nanocomposites for antibacterial purposes are silver and zinc oxides embedded in polymeric matrices [20][21][22]. The antimicrobial activity of silver (Ag) and zinc (Zn) repels or inhibits the initial attachment of bacteria by either exhibiting an anti-biofouling effect or by inactivating cells in contact with surfaces, causing their death [23,24]. Additionally, the antibacterial properties of these agents reduce the risk o...

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“…Moreover, ready-to-eat food, uncooked meat products, vegetables, poultry, and soft cheeses have all been reported as vehicles of listeriosis (Teixeira et al 2007b;Conter et al 2009;Jadhav et al 2012), with ingestion of contaminated food being the main route of transmission for humans (Dussurget 2008). Contamination of food by L. monocytogenes may happen through several distinct routes, such as staff equipment, uncooked materials, or contact surfaces (Møretrø and Langsrud 2004;Teixeira et al 2008). Nevertheless, as far as commercial foodstuff is concerned, contamination by these bacteria is not frequently a consequence of flaws in cleaning and disinfection, but it is due to cross-contamination in the post-processing environment (Ryser and Marth 2007;Latorre et al 2010).…”

Section: Main Foodborne Pathogensmentioning

confidence: 99%

Antibiofilm Strategies in the Food Industry

Teixeira

Rodrigues

2014

Springer Series on Biofilms

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Biofilms in food processing plants represent not only a problem to human health but also cause economic losses by technical failure in several systems. In fact, many foodborne outbreaks have been found to be associated with biofilms. Biofilms may be prevented by regular cleaning and disinfection, but this does not completely prevent biofilm formation. Besides, due to their diversity and to the development of specialized phenotypes, it is well known that biofilms are more resistant to cleaning and disinfection than planktonic microorganisms. In recent years, a considerable effort has been made in the prevention of microbial adhesion and biofilm formation on food processing surfaces and novel technologies have been introduced. In this context, this chapter discusses the main conventional and emergent strategies that have been employed to prevent bacterial adhesion to food processing surfaces and thus to efficiently maintain good hygiene throughout the food industries.

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Adhesion of Listeria monocytogenes to materials commonly found in domestic kitchens

Cited by 24 publications

References 27 publications

Positive Correlation Between the Expression of inlA and inlB Genes of Listeria monocytogenes and Its Attachment Strength on Glass Surface

Positive Correlation Between the Expression of inlA and inlB Genes of Listeria monocytogenes and Its Attachment Strength on Glass Surface

Antimicrobial Activity and Prevention of Bacterial Biofilm Formation of Silver and Zinc Oxide Nanoparticle-Containing Polyester Surfaces at Various Concentrations for Use

Antibiofilm Strategies in the Food Industry

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