Adhesion of perichondrial cells to a polylactic acid scaffold

Abstract

The number of chondrogenic cells available locally is an important factor in the repair process for cartilage defects. Previous studies demonslrdted that the number of transplanted rabbit perichondrial cells (PC) remaining in a cartilage defect in vivo, after being carried into the site in a polylactic acid (PLA) scaffold, declined markedly within two days. This study examined the ability of in vitro culture of PUPLA constructs to enhance subsequent biomechanical stability of the cells and the matrix content in an in vitro screening assay. PC/PLA constructs were analyzed after 1 h, I and 2 weeks of culture. The biomechanical adherence of PC to the PLA scaffold was tested by subjecting the PUPLA constructs to a range of flow velocities (0.25-25 mm/s), spanning the range estimated to occur under conditions of construct insertion in vivo. The adhesion of PC to the PLA carrier was increased significantly by 1 and 2 weeks of incubation, with 25 mm/s flow causing a 57% detachment of cells after 1 h of seeding, but only 7% and 16% after 1 and 2 weeks of culture, respectively (p < 0.001). This adherence was associated with marked deposition of glycosaminoglycan and collagen. These findings suggest that pre-incubation of PC-laden PLA scaffolds markedly enhances the stability of the indwelling cells.

Directing Stem Cell Differentiation into the Chondrogenic Lineage In Vitro

“…These include gelatin [133], chitosan [134], and alginate [135][136][137][138]. Additionally, matrix scaffolds based completely on synthetic materials such as poly (lactic-coglycolic acid), poly (ethylene glycol), poly (epsilon-caprolactone) have also been fabricated [139][140][141][142][143][144]. Nevertheless, it would be preferable to use these as composites with naturally occurring materials.…”

Collagen/hyaluronan membrane as a scaffold for chondrocytes cultivation

“…polylactic acid, polyglycolic acid (Chu et al 1995;Giurea et al 2003;Ueki et al 2003;Hu & Athanasiou 2005), hydroxyapatite (Wu et al 2008), chitosan (Kim et al 2003;Chen et al 2007), and gelatin (Lien et al 2008), to design chondrocyteseeded or cell-free implants for articular cartilage repair. Some of these materials allow formation of the tissue to be repaired that resembles normal cartilage.…”

Evaluation of the growth of chondrocytes and osteoblasts seeded into precision scaffolds fabricated by fused deposition manufacturing

“…In this study, we focused on the fabrication and evaluation of the precision scaffolds molded by FDM for bone and cartilage regeneration using polylactide (PLA) and polycaprolactone (PCL) as starting materials. PLA is a material with good biocompatibility,10 and PLA scaffolds have been used in tissue‐engineered grafts to repair articular cartilage defects 11, 12. PCL is a flexible and structurally stable bioresorbable polymer.…”

“…PLA is a material with good biocompatibility, 10 and PLA scaffolds have been used in tissue-engineered grafts to repair articular cartilage defects. 11,12 PCL is a flexible and structurally stable bioresorbable polymer. It has been applied in tissue-engineered constructs for craniofacial bone repair.…”