Adhesion of Porphyromonas gingivalis and Tannerella forsythia to dentin and titanium with sandblasted and acid etched surface coated with serum and serum proteins – An in vitro study

Eick, Sigrun; Kindblom, C.; Mizgalska, Danuta; Magdon, Anna; Jurczyk, K.; Sculean, Anton; Stavropoulos, Andreas

doi:10.1016/j.archoralbio.2016.11.001

Cited by 12 publications

(12 citation statements)

References 46 publications

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“…No data are currently available from the clinic for dental implants. However, we have recently shown in vitro that expression of gingipains was decreased when P. gingivalis attached to titanium surfaces compared with dentin surfaces . In this study, a decrease of gingipains expression over time was confirmed for bacterial suspension in cell culture medium.…”

Section: Discussionsupporting

confidence: 65%

Gingipains impair attachment of epithelial cell to dental titanium abutment surfaces

et al. 2019

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The study investigated in vitro the effect of Porphyromonas gingivalis and its cysteine proteases (gingipains) on epithelial cell adhesion to titanium–zirconium alloy surfaces. Titanium–zirconium discs with a standard machined (M) or chemically modified hydrophilic surface (modM) were coated with lamin‐5 and incubated with telomerase‐inactivated gingival keratinocytes (TIGK). Three P. gingivalis strains or gingipains were either added simultaneously with TIGK or after TIGK cells were already attached to the disks. Adhered TIGK cells were counted at 24 h. All P. gingivalis strains clearly inhibited adhesion of TIGK cells to M and modM surfaces. Compared with bacteria/gingipain‐free TIGK cell cultures, the number of attached TIGK cells was reduced by about 80% and 60% when P. gingivalis was added simultaneously or after TIGK cells were already attached to the disks (each p < 0.01), respectively. Counts of attached cells were similarly reduced when only gingipains were used. Adhesion molecules of TIGK cells, in particular E‐cadherin, were cleaved by P. gingivalis. In conclusion, P. gingivalis and gingipains interfere with the adhesion of epithelial cells to titanium–zirconium alloy surfaces by cleaving adhesion molecules, while a chemically modified hydrophilic titanium–zirconium alloy surface did not yield any protection. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2549–2556, 2019.

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Section: Discussionsupporting

confidence: 65%

Gingipains impair attachment of epithelial cell to dental titanium abutment surfaces

et al. 2019

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“…P. gingivalis cysteine proteases (gingipains) were always expressed both at implants and at teeth, which corroborates with results of our recent in vitro study using titanium and dentine disks. 32 The highest mRNA expression related to sod was found for kgp , followed by rgpA and rgpB . Level of Kgp was determined as being up to 10 nM in periodontium, 33 those of Arg-gingipains up to 1.5 μM.…”

Section: Discussionmentioning

confidence: 94%

“…Recently an in vitro study found that miropin expression was lower at titanium implants than at dentine disks. 32 However, the miropin expression by T. forsythia adhered either to the dentin or titanium surface exceeded up to 500-fold the expression of proteases (miropsin-1, miropsin-2, mirolase. 32 It is clear that more research is needed on T. forsythia , not only as the periodontal pathogen, but also a potentially pathogenic member of the peri-implant biofilm consortium.…”

Section: Discussionmentioning

confidence: 95%

In vivo expression of proteases and protease inhibitor, a serpin, by periodontal pathogens at teeth and implants

Eckert

Mizgalska

Sculean

et al. 2018

Molecular Oral Microbiology

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Porphyromonas gingivalis and Tannerella forsythia secrete proteases, gingipains and KLIKK-proteases. In addition, T. forsythia produces a serpin (miropin) with broad inhibitory spectrum. The aim of this pilot study was to determine the level of expression of miropin and individual proteases in vivo in periodontal and peri-implant health and disease conditions. Biofilm and gingival crevicular fluid (GCF)/ peri-implant sulcular fluid (PISF) samples were taken from healthy tooth and implant sites (n = 10), gingivitis and mucositis sites (n = 12), and periodontitis and peri-implantitis sites (n = 10). Concentration of interleukin-8 (IL-8), IL-1β and IL-10 in GCF was determined by enzyme-linked immunosorbent assay. Loads of P. gingivalis and T. forsythia and the presence of proteases and miropin genes were assessed in biofilm by quantitative PCR, whereas gene expression was estimated by quantitative RT-PCR. The presence of P. gingivalis and T. forsythia, as well as the level of IL-8 and IL-1β, were associated with disease severity in the periodontal and peri-implant tissues. In biofilm samples harboring T. forsythia, genes encoding proteases were found to be present at 72.4% for karilysin and 100% for other KLIKK-protease genes and miropin. At the same time, detectable mRNA expression of individual genes ranged from 20.7% to 58.6% of samples (for forsylisin and miropsin-1, respectively). In comparison with the T. forsythia proteases, miropin and the gingipains were highly expressed. The level of expression of gingipains was associated with those of miropin and certain T. forsythia proteases around teeth but not implants. Cumulatively, KLIKK-proteases and especially miropin, might play a role in pathogenesis of both periodontal and peri-implant diseases.

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“…Previous studies have highlighted the indispensable role that implant surface roughness plays in bacterial adhesion [34]. Treatments, such as laser treatment, have gained popularity in dental therapy due to their benefits of low thermal damage; radiofrequency treatment (cold plasma) has also been shown to be capable of inhibiting microbial activity [42,43]. Bacterial colonization occurs during implantation or after osseous integration and interferes with the oral environment and material surface characteristics [44].…”

Section: Discussionmentioning

confidence: 99%

Glow Discharge Plasma Treatment on Zirconia Surface to Enhance Osteoblastic-Like Cell Differentiation and Antimicrobial Effects

et al. 2020

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Peri-implantitis is the pathological condition of connective tissue inflammation and the progressive loss of supporting bone around dental implants. One of the primary causes of peri mucositis evolving into peri-implantitis is bacterial infection, including infection from Porphyromonas gingivalis. Enhancing the surface smoothness of implants helps to prevent P. gingivalis adhesion to the implant’s surface. Interaction analyses between bacteria and the surface roughness of zirconia (Zr) discs subjected to a glow discharge plasma (GDP) treatment compared with non-plasma-treated autoclaved control Zr discs were done. Examinations of the material prosperities revealed that the GDP-treated Zr group had a smoother surface for a better wettability. The GDP-treated Zr discs improved the proliferation of the osteoblast-like cells MG-63, and the osteoblastic differentiation was assessed through alkaline phosphatase detection and marker gene bone sialoprotein (Bsp) and osteocalcin (OC) induction. Scanning electron microscopy demonstrated a relatively low P. gingivalis adhesion on GDP-treated Zr disks, as well as lower colonization of P. gingivalis compared with the control. Our findings confirmed that the GDP treatment of Zr discs resulted in a significant reduction of P. gingivalis adhesion and growth, demonstrating a positive correlation between surface roughness and bacteria adhesion. Therefore, the GDP treatment of Zr dental implants can provide a method for reducing the risk of peri-implantitis.

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Adhesion of Porphyromonas gingivalis and Tannerella forsythia to dentin and titanium with sandblasted and acid etched surface coated with serum and serum proteins – An in vitro study

Cited by 12 publications

References 46 publications

Gingipains impair attachment of epithelial cell to dental titanium abutment surfaces

Gingipains impair attachment of epithelial cell to dental titanium abutment surfaces

In vivo expression of proteases and protease inhibitor, a serpin, by periodontal pathogens at teeth and implants

Glow Discharge Plasma Treatment on Zirconia Surface to Enhance Osteoblastic-Like Cell Differentiation and Antimicrobial Effects

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