Adhesion properties of cell surface proteins in Lactobacillus strains in the GIT environment

Yu, Yixing; Zong, Manli; Lao, Lifeng; Wen, Jianzhu; Pan, Daodong; Wu, Zhen

doi:10.1039/d1fo04328e

Cited by 16 publications

(6 citation statements)

References 96 publications

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“…The tight junction (TJ) between colonic epithelial cells is an important structure to maintain intestinal barrier function. At the same time, mucus layer is also an important part of intestinal barrier function ( 24 , 25 ). It is the first line of defense against symbiotic microorganisms and invasive pathogens.…”

Section: Resultsmentioning

confidence: 99%

Collagen peptide promotes DSS-induced colitis by disturbing gut microbiota and regulation of macrophage polarization

Cui²,

Feng³

et al. 2022

Front. Nutr.

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Ulcerative colitis (UC) is an inflammatory bowel disease caused by mucosal immune system disorder, which has increased steadily all over the world. Previous studies have shown that collagen peptide (CP) has various beneficial biological activities, it is not clear whether the effect of CP on UC is positive or negative. In this study, 2.5% dextran sulfate sodium (DSS) was used to establish acute colitis in mice. Our results suggested that CP supplementation (200, 400 mg/kg/day) promoted the progression of colitis, increased the expression of inflammatory factors and the infiltration of colonic lamina propria macrophages. Gut microbiota analysis showed the composition changed significantly and inflammation promoted bacteria was after CP treatment. Meanwhile, the effect of CP on macrophage polarization was further determined in Raw264.7 cell line. The results showed that CP treatment could increase the polarization of M1 macrophages and promote the expression of inflammatory factors. In conclusion, our results showed that CP treatment could disrupt the gut microbiota of host, promote macrophage activation and aggravate DSS-induced colitis. This may suggest that patients with intestinal inflammation should not take marine derived CP.

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Section: Resultsmentioning

confidence: 99%

Collagen peptide promotes DSS-induced colitis by disturbing gut microbiota and regulation of macrophage polarization

Cui²,

Feng³

et al. 2022

Front. Nutr.

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“…Surface proteins from P. freudenreichii have immunomodulatory properties in peripheral blood mononuclear cells and involve in adhesion to colon cells 14 , 20 . Surface proteins show immunomodulatory effects in the intestine 21 , 22 , which suggests that surface proteins obtained from probiotics may be developed as a treatment for immune-related diseases, such as inflammation, after evaluating their clinical effects. In this study, surface proteins of MJ2 inhibited the expression of NFATc1 and also of Ctsk , Calcr , and Atp6vod2 which engage in bone resorption by osteoclast 23 .…”

Section: Discussionmentioning

confidence: 99%

Surface proteins of Propionibacterium freudenreichii MJ2 inhibit RANKL-induced osteoclast differentiation by lipocalin-2 upregulation and lipocalin-2-mediated NFATc1 inhibition

Yeom,

Ma,

Yim

et al. 2023

Sci Rep

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Osteoclasts degrade bone and osteoclast differentiation has been implicated in bone destruction in rheumatoid arthritis. The dairy bacterium Propionibacterium freudenreichii MJ2 (MJ2) isolated from raw milk inhibits osteoclast differentiation and ameliorates collagen-induced arthritis. This study aimed to investigate the inhibitory effect of the surface proteins of MJ2 on receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation and explain the underlying mechanism. The murine macrophage cell line RAW 264.7 was used to study the inhibition of osteoclast differentiation. The surface proteins significantly inhibited RANKL-induced osteoclast differentiation in a protein concentration-dependent manner by inhibiting the expression of genes and proteins related to osteoclast differentiation. RNA microarray analysis showed that the surface proteins significantly upregulated lipocalin-2 (lcn2) expression. In addition, they downregulated c-fos and NFATc1 and inhibited the expression of NFATc1-downstream genes Atp6v0d2, Calcr, and Ctsk. siRNA silencing of lcn2 decreased the extent of surface protein inhibition on osteoclast differentiation, suggesting that lcn2 plays an important role in the inhibition of RANKL-induced osteoclast differentiation. In conclusion, surface proteins of MJ2 show inhibitory effects on RANKL-induced osteoclast differentiation by upregulating lcn2 expression, in turn downregulating NFATc1, leading to the inhibition of NFATc1-downstream osteoclastogenesis-related gene expression.

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“…Lactobacillus strain, a member of lactic acid bacteria, is characterized in fermentation of food and can enhance the intestinal barrier function as the probiotic strain. Lactobacillus adheres and aggregates in the gastrointestinal tract to promote maturity of biofilm and the extracellular matrix secreting through the signal molecules in the quorum sensing system [24]. Therefore, adhesion properties of cell surface proteins derived from Lactobacillus strains provides a valuable vehicle to target mucus containing tissues.…”

Section: Discussionmentioning

confidence: 99%

Study effect of MAPA-VIP on control of allergic asthma pathophysiology

Jia,

Yang

2023

pdia

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Introduction: Asthma is a pulmonary disease and its pathogenesis is involved with immune cells and related signalling pathways. Alpha-alumina is material for therapy applications and mucus adhesion promoting protein is cellsurface protein. Vasoactive intestinal peptide (VIP) exerts immunomodulation. Therefore, the drug delivery system and target binding molecule could be applicable for treatment of asthma. Material and methods: VIP-MapA-α-alumina was administered to asthmatic mice. Then, eosinophil percentage, IgE, IL-4, IL-5, and IL-13 levels, GATA3, and MUC5AC gene expression, ROS and lung histopathology were studied. Results: Eosinophil percentage, IgE, IL-4, IL-5, IL-13, and ROS levels, expression of GATA3 and MUC5AC genes, goblet cell hyperplasia, mucus hyper-production, perivascular and peribronchial inflammation were decreased in VIP and VIP-MapA treated groups and treatment with VIP-MapA has a stronger effect than VIP alone. Conclusions: The delivery system of VIP carrying to the lung with the use of MapA as an adhesion molecule, could easily carry VIP and led to penetration of this component to the mucus and reach bronchial cells and present an effective, strong, and long-acting effect on therapy of asthma.

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Adhesion properties of cell surface proteins in Lactobacillus strains in the GIT environment

Abstract: Lactic acid bacteria (LAB) play an essential role in the epithelial barrier and the gut immune system. It can antagonize pathogens by producing antimicrobial substances like bacteriocins, and compete with...

Cited by 16 publications

References 96 publications

Collagen peptide promotes DSS-induced colitis by disturbing gut microbiota and regulation of macrophage polarization

Collagen peptide promotes DSS-induced colitis by disturbing gut microbiota and regulation of macrophage polarization

Surface proteins of Propionibacterium freudenreichii MJ2 inhibit RANKL-induced osteoclast differentiation by lipocalin-2 upregulation and lipocalin-2-mediated NFATc1 inhibition

Study effect of MAPA-VIP on control of allergic asthma pathophysiology

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