Adhesion to the digestive mucosa is not sufficient for durable persistence of different<i>Lactobacillus fermentum</i>strains in the digestive tract of mice

Hautefort, Isabelle

doi:10.1080/089106000435590

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…The remaining isolates failed to produce biofilm using the in vitro model. Our results were in agreement with the previous works, showing an extensive variation in biofilm formation among the isolates [32,27] and supported the notion of no correlation between in vitro attachment and biofilm formation abilities (r=0.11, P=0.51). However, the high ratios of these two abilities were observed only in one isolate, i.e., L. plantarum strain 42.…”

Section: Discussionsupporting

confidence: 93%

Correlation of Biofilm Formation and Caco-2 Cell Attachment Properties in Colonization Ability of Acid-Bile Resistant Fecal Lactobacillus plantarum Isolates

Rohani

Papizadeh

Pourshafie

2018

JoMMID

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Introduction:The strain-specific capabilities of lactobacilli are critical for gut colonization. In this study, we evaluated various colonization determinants of 42 fecal Lactobacillus plantarum isolates from the healthy human fecal samples. Methods: We investigated the attachment to the Caco-2 cell line, biofilm formation ability and cell surface activity of the isolates. Such properties were comparatively studied, and the regression between these features was statistically analyzed. Results: Among our results 18 (42.9%) were non-adhesive, 11 (26.2%) moderate adhesive and 13 (31%) strongly adhesive. The results showed that some isolates were significantly capable of biofilm formation. The highest rate of auto-aggregation was recorded for L. plantarum isolate RPR 240 (58%). Conclusion: Our results revealed a high degree of variability among colonization characteristics of the L. plantarum isolates indicating strain-specificity. Also, the colonization properties of the potential probiotic isolates had no significant correlation with each other. Our results confirmed the necessity of further in vivo colonization assays for selection of probiotic candidates. J Med Microbiol Infec Dis, 2018, 6 (1): 13-19.

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Section: Discussionsupporting

confidence: 93%

Correlation of Biofilm Formation and Caco-2 Cell Attachment Properties in Colonization Ability of Acid-Bile Resistant Fecal Lactobacillus plantarum Isolates

Rohani

Papizadeh

Pourshafie

2018

JoMMID

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“…Our results show that certain strains are able to persist and compete regardless of diet and competing LAB, and, in some cases, competing LAB can fortuitously support persistence of some strains. Hautefort et al (29) found that the presence of a resident microbiota had a positive effect on the adhesion capacity of inoculated strains. The inoculation of, or background presence of, a mixed microbiota may create a synergistic environment that assists some strains in becoming established.…”

Section: Discussionmentioning

confidence: 99%

“…It can be difficult to predict how these in vitro characteristics translate to the corresponding activity in vivo. For example, Hautefort et al (29) reported that several strains were able to persist within mice without adhesion to the GIT lining, indicating that in vitro adhesion assays may be redundant. Gardiner et al (22) argued that performance in the gut can be determined only truly in vivo, suggesting that the time and expense of assays such as those described here may be worthwhile.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus Strain Ecology and Persistence within Broiler Chickens Fed Different Diets: Identification of Persistent Strains

Stephenson

Moore

Allison

2010

Appl Environ Microbiol

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Lactobacilli are autochthonous residents in the chicken gastrointestinal tract, where they may potentially be used as probiotics, competitive exclusion agents, or delivery vehicles. The aim of this study was to use an in vivo model to investigate the effect of diet and competing lactic acid bacteria on the colonization of inoculated Lactobacillus strains, with the goal of identifying strains which can consistently colonize or persist for an extended period of several weeks. Chicken-derived Lactobacillus strains were genetically marked with rifampin resistance and administered on day 0 to chickens fed either a normal commercial diet or a specially formulated high-protein diet. Chickens fed the high-protein diet were also coinoculated with two different mixes of additional lactic acid bacteria. Enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) was used to identify rifampin-resistant isolates recovered from chickens. Three strains, belonging to the species Lactobacillus agilis, Lactobacillus crispatus, and Lactobacillus vaginalis, were commonly reisolated from the chickens on both diets at days 21 and 42. The ability of these strains to persist was confirmed in a second chicken trial. All three strains persisted throughout the production period in the chickens fed a commercial diet, while only the L. agilis and L. vaginalis strains persisted in the chickens fed the high-protein diet. In both in vivo trials, competing lactic acid bacteria modified representation of the strains recovered, with all three stains capable of competing in the presence of one or both mixes of coinoculated strains. The in vivo model successfully identified three persistent strains that will be characterized further.The ecology of the chicken gastrointestinal tract (GIT) has been studied in depth using both culture-dependent (5,7,21,40) and -independent methods (2,3,7,33,54). These studies have revealed that lactobacilli are autochthonous residents in chickens, where they predominate in the proximal GIT and are present but less abundant within the distal GIT (52). The most commonly identified Lactobacillus species are Lactobacillus crispatus, Lactobacillus reuteri, and Lactobacillus salivarius (1,7,15,26,28). A detailed understanding of the relationship between these bacteria and their host under different dietary and environmental conditions will facilitate the development of lactobacilli for various applications directed toward increasing broiler production efficiency and improving chicken health.Since the withdrawal of antimicrobial growth promoters from chicken feed in Europe, the incidence of necrotic enteritis (NE) has increased (9, 51). Consequently, there is a need to develop alternative methods for controlling the causative agent of NE, Clostridium perfringens, in the chicken GIT. Lactobacilli are excellent candidates for alternative control methods due to their autochthonous nature and dominance of the upper GIT microbiota, particularly within the small intestine where NE occurs. Their potential utility ...

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“…This was suggested to be caused by the specificity of attachment site and the removal of mucus (Bezkorovainy 2001). Moreover, Hautefort et al. (2000) also reported that adhesion was not sufficient to ensure the persistence of L. fermentum strains in the digestive tract of mice.…”

Section: Discussionmentioning

confidence: 99%

Adherence and colonization properties of Lactobacillus rhamnosus TB1, a broiler chicken isolate

Bouzaine

Dauphin

Thonart

et al. 2005

Lett Appl Microbiol

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T . B O U Z A I N E , R . D . D A U P H I N , P H . T H O N A R T , M . C . U R D A C I A N D M . H A M D I . 2005.Aims: Selected lactic acid bacteria (LAB) isolated from intestinal tract of chicken have been studied in order to investigate their ability to adhere in vitro to Basement Membrane Matrigel (BMM). A selected strain showing a good adherence in BMM test was used for in vivo colonization assays. Methods and Results: In vitro assessment of adhesion of broiler chicken isolates was performed using BMM assay. Among LAB strains tested, Lactobacillus rhamnosus TB1 showed a good adherence that was comparable to the one of an Escherichia coli EPEC strain used as positive control. For in vivo colonization assays this strain was fluorescently stained with the carboxyfluorescein diacetate succinimidyl ester (cFDA-SE) thus allowing its detection in different layers of intestinal tract after inoculation in broiler chicken. Further, stained L. rhamnosus were found with a highest value in rectum, jejunum and ileum both 3 and 24 h after administration. Conclusions: BMM assay is a quick method to test in vitro adhesion properties of bacterial strains and cFDA-SE-stained bacteria may be considered as an alternative method to test in vivo adhesion and colonization properties. Significance and Impact of the Study: Lactobacillus rhamnosus TB1 was therefore showed to be able to adhere strongly in vitro to BMM and in vivo to intestinal epithelial cells of chicken and may be considered as a potential probiotic for chicken.

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Adhesion to the digestive mucosa is not sufficient for durable persistence of differentLactobacillus fermentumstrains in the digestive tract of mice

Cited by 8 publications

References 24 publications

Correlation of Biofilm Formation and Caco-2 Cell Attachment Properties in Colonization Ability of Acid-Bile Resistant Fecal Lactobacillus plantarum Isolates

Correlation of Biofilm Formation and Caco-2 Cell Attachment Properties in Colonization Ability of Acid-Bile Resistant Fecal Lactobacillus plantarum Isolates

Lactobacillus Strain Ecology and Persistence within Broiler Chickens Fed Different Diets: Identification of Persistent Strains

Adherence and colonization properties of Lactobacillus rhamnosus TB1, a broiler chicken isolate

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