Adhesional Function of Canine Mammary Gland Tumor Cells Expressing Sialyl Lewis X

Abstract

ABSTRACT. The adhesional function to blood vessel endothelial cells was evaluated in 2 cultured canine mammary gland tumor cells, CHMp and CHMm, derived from the primary and the metastatic lesion of the same patient. They exhibited negative (CHMp) and positive (CHMm) expression of sialyl Lewis X [sLe(x)], respectively. In the cell adhesion assay, the number of attached CHMm cells to human umbilical vein endothelial cells which expressed activated E-selectin was significantly increased. The process of cell-cell adhesion under flow conditions was observed only in CHMm cells. CHMm cells were revealed to have the adhesional ability to blood vessel endothelial cells and this adhesional ability was thought to be induced by the sLe(x)-E-selectin binding.KEY WORDS: E-selectin, mammary gland tumor, sLe(x).

Bauhinia purprea agglutinin‐modified liposomes for human prostate cancer treatment

“…PSA, a serine proteinase glycoprotein, is a suitable marker for detecting prostate cancer, because the amount of PSA in the bloodstream of the patient begins to increase with the development of prostate cancer . In addition, it is known that some tumor‐associated carbohydrate antigens such as sialyl LewisX (sLeX) can be indicators of tumor malignancy in some types of cancer, including prostate cancer . These findings point to the importance of cell‐surface sugar chains in tumor development and the possibility of using them as targets for drug delivery and molecular imaging.…”

Modulation of Cellular Adhesion by Glycoengineering

“…13 For instance, a recent surgical note demonstrated that for cultured cells derived from primary (CHMp) and metastatic (CHMm) lesions of a canine mammary gland tumor from the same animal, adhesion to human umbilical vein endothelial cells that express activated E-selectin was dramatically enhanced in the case of CHMm cells. 14 Similarly, expression levels of R2,3-linked sialic acid residues correlated with the metastatic potential of human gastric cancers. 15 The R2-6 sialyltransferase enzyme (ST6Gal-I) is upregulated by oncogenes such as ras, leading to dramatically increased R2-6 sialylation of the β 1 subunit of integrins, and β 1 from adenocarcinomas exhibits increased R2-6 sialylation relative to normal epithelial cells.…”

“…Sialyl Lewis X (SLe X ) and A (SLe A ), tetrasaccharide epitopes that bind to selectins (Figure 1B), are overexpressed on tumor cells and correlate with poor prognosis 13. For instance, a recent surgical note demonstrated that for cultured cells derived from primary (CHMp) and metastatic (CHMm) lesions of a canine mammary gland tumor from the same animal, adhesion to human umbilical vein endothelial cells that express activated E-selectin was dramatically enhanced in the case of CHMm cells 14. Similarly, expression levels of α2,3-linked sialic acid residues correlated with the metastatic potential of human gastric cancers 15.…”

“…Members of two key protein families, the selectins and the integrins, mediate leukocyte rolling and firm adhesion−essential steps in extravasation. − Sialyl Lewis X (SLe X a Abbreviations: BSA, bovine serum albumin; DMB, 1,2-diamino-4,5-methylene-dioxybenzene; ECM, extra cellular matrix; ELISA, enzyme-linked immunosorbent assay; PBS, phosphate buffered saline; PSGL-1, P-selectin glycoprotein ligand-1; SDS, sodium dodecyl sulfate; SLe A , sialyl Lewis A; SLe X , sialyl Lewis X; ST6Gal-I, α2−6 sialyltransferase enzyme. ) and A (SLe A ), tetrasaccharide epitopes that bind to selectins (Figure B), are overexpressed on tumor cells and correlate with poor prognosis . For instance, a recent surgical note demonstrated that for cultured cells derived from primary (CHMp) and metastatic (CHMm) lesions of a canine mammary gland tumor from the same animal, adhesion to human umbilical vein endothelial cells that express activated E-selectin was dramatically enhanced in the case of CHMm cells . Similarly, expression levels of α2,3-linked sialic acid residues correlated with the metastatic potential of human gastric cancers .…”

Stage-specific embryonic antigen: determining expression in canine glioblastoma, melanoma, and mammary cancer cells

“…The expression of SSEAs has been previously examined in embryonic and adult canine mesenchymal stem cells [15], and the distribution and function of SSEA-1 have been examined in metastatic canine mammary tumor cells [6]. However, the expression of SSEAs has not been examined systematically in canine cancer cells.…”