Adhesive cell cultivation on polymer particle having grafted epoxy polymer chain

Yasuda, Masahiro; Kunieda, Hiroshi; Ono, Kentaro; Ogino, Hiroyasu; Iwasaki, Takashi; Hiramoto, Masaki; Glomm, Wilhelm R.; Hirabayashi, Yukio; Aizawa, Shin

doi:10.1016/j.tice.2010.12.007

Cited by 6 publications

(17 citation statements)

References 29 publications

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“…Previously, we established a novel 3D culture system using polymer particles with grafted epoxy polymer chains for cell immobilization [23,24]. In our 3D culture system, MS-5 cells are used as stromal cells [23], and HSC proliferation and differentiation are maintained for significantly longer, recapitulating more closely the natural hematopoietic niche compared with traditional 2D culture systems.…”

Section: Discussionmentioning

confidence: 99%

“…We recently developed novel polymer particles with grafted epoxy polymer chains to support cell immobilization in a new 3D cell cultivation system [23][24][25]. The base polymer particles were synthesized by suspension polymerization of acrylic monomer and 2,2'-azobis[N-(2-propenyl)-2methylpropionamide] (APMPA), and the epoxy polymer chain was extended from the particle surface by graft polymerization.…”

Section: Open Accessmentioning

confidence: 99%

“…Polymer particles with grafted epoxy polymer chains were prepared as described previously [24]. For graft polymerization, GMA and MA were used at a ratio of 4:1 (w/w), respectively, and the resulting particles were washed in a funnel with a 40  volume of distilled water and methanol.…”

Section: Preparation Of Cell Support With Grafted Polymer Chainsmentioning

confidence: 99%

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In vitro chemosensitivity study of human leukemic cells in a three-dimensional bone marrow culture system

Fukino¹,

Harada²,

Tsuboi³

et al. 2016

J Hematol Ther.

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In the bone marrow, hematopoietic stem cell proliferation and differentiation are regulated by the hematopoietic microenvironment. This is reflected by the ability of the bone marrow microenvironment to alter the growth of leukemic cells, and protect leukemic cells from anticancer agents. Previously, we established a three-dimensional (3D) bone marrow culture system that maintained normal hematopoiesis, including prolongation of hematopoietic stem cell proliferation and differentiation. In the present study, we analyzed the effects of the anti-cancer drug, cytarabine, on the human leukemic cell line (K562) co-cultured with stromal cells in the 3D system. Comparisons were made with K562 cells treated with cytarabine in suspension or grown on a two-dimensional stromal cell monolayer (2D). We demonstrated that K562 cells cultured in the 3D system were more resistant to cytarabine treatment compared with cells grown in 2D or in suspension. Furthermore, there was a significant increase in the number of K562 cells in G 0 /G 1 phase in 3D culture compared with cells grown in 2D or suspension cultures. These findings suggest that the differential response to cytarabine treatment in 3D culture may be related to the cell cycle period, which was modulated by stromal cells in the 3D microenvironment. Thus, the 3D culture system may be a valuable new tool for investigating leukemic cell-stromal cell interactions and the leukemic cell response to anti-cancer agents in vitro.

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Section: Discussionmentioning

confidence: 99%

Section: Open Accessmentioning

confidence: 99%

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In vitro chemosensitivity study of human leukemic cells in a three-dimensional bone marrow culture system

Fukino¹,

Harada²,

Tsuboi³

et al. 2016

J Hematol Ther.

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“…In this report, we show that we can capture some of the complexity of neural networks in the brain through interfacing in vitro neural cell cultures with surface-grafted, non-conducting, polymer particles (PPs) to create neural networks with 3D topology. Previously, these PPs have been successfully employed as microenvironments for creating 3D bone marrow culture systems, which have been used for both haematopoietic stem cell studies [18–20] and chemosensitivity studies [21]. In the present study, we report the structuring of neural networks using PPs combined with 3D MEAs for electrophysiological network measurements and show how the resulting structural and functional network traits relate to a small-world network topology.…”

Section: Introductionmentioning

confidence: 93%

Formation of neural networks with structural and functional features consistent with small-world network topology on surface-grafted polymer particles

et al. 2019

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In vitro electrophysiological investigation of neural activity at a network level holds tremendous potential for elucidating underlying features of brain function (and dysfunction). In standard neural network modelling systems, however, the fundamental three-dimensional (3D) character of the brain is a largely disregarded feature. This widely applied neuroscientific strategy affects several aspects of the structure–function relationships of the resulting networks, altering network connectivity and topology, ultimately reducing the translatability of the results obtained. As these model systems increase in popularity, it becomes imperative that they capture, as accurately as possible, fundamental features of neural networks in the brain, such as small-worldness. In this report, we combine in vitro neural cell culture with a biologically compatible scaffolding substrate, surface-grafted polymer particles (PPs), to develop neural networks with 3D topology. Furthermore, we investigate their electrophysiological network activity through the use of 3D multielectrode arrays. The resulting neural network activity shows emergent behaviour consistent with maturing neural networks capable of performing computations, i.e. activity patterns suggestive of both information segregation (desynchronized single spikes and local bursts) and information integration (network spikes). Importantly, we demonstrate that the resulting PP-structured neural networks show both structural and functional features consistent with small-world network topology.

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“…We recently developed novel polymer particles with grafted epoxy polymer chains for use in establishing a new 3D cell cultivation system. 31 The particles, composed of base polymer particles and grafted polymer chains, were used as a support for cell immobilization. The base polymer particles were synthesized by suspension polymerization of acrylic monomer and 2,2 0 -azobis[N-(2-propenyl)-2-methylpropionamide] (APMPA), and the epoxy polymer chain was extended from the particle surface by graft polymerization.…”

Section: Introductionmentioning

confidence: 99%

Novel three-dimensional long-term bone marrow culture system using polymer particles with grafted epoxy-polymer-chains supports the proliferation and differentiation of hematopoietic stem cells

Hirabayashi

Hatta²,

Jin³

et al. 2011

Exp Biol Med (Maywood)

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Hematopoiesis occurs in the bone marrow, where primitive hematopoietic cells proliferate and differentiate in close association with a three-dimensional (3D) hematopoietic microenvironment composed of stromal cells. We examined the hematopoietic supportive ability of stromal cells in a 3D culture system using polymer particles with grafted epoxy polymer chains. Umbilical cord blood-derived CD34(+) cells were co-cultivated with MS-5 stromal cells. They formed a 3D structure in the culture dish in the presence of particles, and the total numbers of cells and the numbers of hematopoietic progenitor cells, including colony-forming unit (CFU)-Mix, CFU-granulocyte-macrophage, CFU-megakaryocyte and burst-forming unit-erythroid, were measured every seven days. The hematopoietic supportive activity of the 3D culture containing polymer particles and stromal cells was superior to that of 2D culture, and allowed the expansion and maintenance of hematopoietic progenitor cells for more than 12 weeks. Various types of hematopoietic cells, including granulocytes, macrophages and megakaryocytes at different maturation stages, appeared in the 3D culture, suggesting that the CD34(+) cells were able to differentiate into a range of blood cell types. Morphological examination showed that MS-5 stromal cells grew on the surface of the particles and bridged the gaps between them to form a 3D structure. Hematopoietic cells slipped into the 3D layer and proliferated within it, relying on the presence of the MS-5 cells. These results suggest that this 3D culture system using polymer particles reproduced the hematopoietic phenomenon in vitro, and might thus provide a new tool for investigating hematopoietic stem cell-stromal cell interactions.

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Adhesive cell cultivation on polymer particle having grafted epoxy polymer chain

Cited by 6 publications

References 29 publications

In vitro chemosensitivity study of human leukemic cells in a three-dimensional bone marrow culture system

In vitro chemosensitivity study of human leukemic cells in a three-dimensional bone marrow culture system

Formation of neural networks with structural and functional features consistent with small-world network topology on surface-grafted polymer particles

Novel three-dimensional long-term bone marrow culture system using polymer particles with grafted epoxy-polymer-chains supports the proliferation and differentiation of hematopoietic stem cells

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