Adhesive, Stretchable, and Spatiotemporal Delivery Fibrous Hydrogels Harness Endogenous Neural Stem/Progenitor Cells for Spinal Cord Injury Repair

Chen, Zhenni; Zhang, Haimin; Fan, Cunyi; Zhuang, Yan; Yang, Wen; Chen, Yanyan; Shen, He; Xiao, Zhifeng; Zhao, Yannan; Li, Xiaoran; Dai, Jianwu

doi:10.1021/acsnano.1c06892

Cited by 57 publications

(48 citation statements)

References 65 publications

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“…Tuj-1 and MAP2 are markers for early neurons and mature neurons, respectively [ 44 ]. We used SYN and PSD95 as indicators of synapses [ 33 , 35 , 42 ].…”

Section: Resultsmentioning

confidence: 99%

Three-dimensional-printed collagen/chitosan/secretome derived from HUCMSCs scaffolds for efficient neural network reconstruction in canines with traumatic brain injury

Liu

Zhang

Pan³

et al. 2022

Regenerative Biomaterials

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The secretome secreted by stem cells and bioactive material has emerged as a promising therapeutic choice for traumatic brain injury (TBI). We aimed to determine the effect of 3D-printed collagen/chitosan/secretome derived from human umbilical cord blood mesenchymal stem cells scaffolds (3D-CC-ST) on the injured tissue regeneration process. 3D-CC-ST was performed using 3D printing technology at a low temperature (-20 °C), and the physical properties and degeneration rate were measured. The utilization of low temperature contributed to a higher cytocompatibility of fabricating porous 3D architectures that provide a homogeneous distribution of cells. Immediately after the establishment of the canine TBI model, 3D-CC-ST and 3D-CC (3D-printed collagen/chitosan scaffolds) were implanted into the cavity of TBI. Following implantation of scaffolds, neurological examination and motor evoked potential detection were performed to analyse locomotor function recovery. Histological and immunofluorescence staining were performed to evaluate neuro-regeneration. The group treated with 3D-CC-ST had good performance of behavior functions. Implanting 3D-CC-ST significantly reduced the cavity area, facilitated the regeneration of nerve fibers and vessel reconstruction, and promoted endogenous neuronal differentiation and synapse formation after TBI. The implantation of 3D-CC-ST also markedly reduced cell apoptosis and regulated the level of systemic inflammatory factors after TBI.

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“…Tuj-1 and MAP2 are markers for early neurons and mature neurons, respectively [ 44 ]. We used SYN and PSD95 as indicators of synapses [ 33 , 35 , 42 ].…”

Section: Resultsmentioning

confidence: 99%

Three-dimensional-printed collagen/chitosan/secretome derived from HUCMSCs scaffolds for efficient neural network reconstruction in canines with traumatic brain injury

Liu

Zhang

Pan³

et al. 2022

Regenerative Biomaterials

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“…Furthermore, the introduction of factor-1α (SDF1α) and paclitaxel (PTX) by the electrohydrodynamic jet printing method enables hydrogels with spatiotemporal delivery ability, which can recruit endogenous NSCs in situ for SCI repair (Figure 1A). 50 On one hand, the encapsulation of SDF1α facilitates the migration of NSCs. On the other hand, the encapsulation of PTX facilitates the differentiation of NSCs.…”

Section: Neural Stem Cellsmentioning

confidence: 99%

Section: Pluripotent Stem Cellsmentioning

confidence: 99%

Current treatments after spinal cord injury: Cell engineering, tissue engineering, and combined therapies

Shen

Cao

et al. 2022

Smart Medicine

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Both traumatic and non‐traumatic spinal cord injuries (SCIs) can be categorized as damages done to our central nervous system (CNS). The patients' physical and mental health may suffer greatly because of traumatic SCI. With the widespread use of motor vehicles and increasingly aged population, the occurrence of SCI is more frequent than before, creating a considerable burden to global public health. The regeneration process of the spinal cord is hampered by a series of events that occur following SCI like edema, hemorrhage, formation of cystic cavities, and ischemia. An effective strategy for the treatment of SCI and functional recovery still has not been discovered; however, recent advances have been made in bioengineering fields that therapies based on cells, biomaterials, and biomolecules have proved effective in the repair of the spinal cord. In the light of worldwide importance of treatments for SCI, this article aims to provide a review of recent advances by first introducing the physiology, etiology, epidemiology, and mechanisms of SCI. We then put emphasis on the widely used clinical treatments and bioengineering strategies (cell‐based, biomaterial‐based, and biomolecule‐based) for the functional regeneration of the spinal cord as well as challenges faced by scientists currently. This article provides scientists and clinicians with a comprehensive outlook on the recent advances of preclinical and clinical treatments of SCI, hoping to help them find keys to the functional regeneration of SCI.

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“…Certain special factors have been added to protein hydrogels to increase cell signalling. Using a novel approach to protein binding and assembly, Chen et al [203] added stromal cell-derived factor-1α (SDF1α)/paclitaxel (PTX) to collagen (Col-FB) fibre hydrogel (Figure 7a); Col-FB fibre hydrogel induced the migration of endogenous nerve stem/progenitor cells (NSPCs) over a longer distance (Figure 7b-d). After PTX incorporation, Col-FB fibre hydrogel can effectively promote the differentiation of NSPCs neurons (Figure 7e-g).…”

Section: Nerve Repairmentioning

confidence: 99%

Natural polymer‐based adhesive hydrogel for biomedical applications

Long

Xie

2022

Biosurface and Biotribology

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Hydrogel is a polymer network system that can form a hydrophilic three‐dimensional network structure through different cross‐linking methods. In recent years, hydrogels have received considerable attention due to their good biocompatibility and biodegradability by introducing different cross‐linking mechanisms and functional components. Compared with synthetic hydrogels, natural polymer‐based hydrogels have low biotoxicity, high cell affinity, and great potential for biomedical fields; however, their mechanical properties and tissue adhesion capabilities have been unable to meet clinical requirements. In recent years, many efforts have been made to solve these issues. In this review, the recent progress in the field of natural polymer‐based adhesive hydrogels is highlighted. The authors first introduce the general design principles for the natural polymer‐based adhesive hydrogels being used as excellent tissue adhesives and the challenges associated with their design. Next, their usages in biomedical applications are summarised, such as wound healing, haemostasis, nerve repair, bone tissue repair, cartilage tissue repair, electronic devices, and other tissue repairs. Finally, the potential challenges of natural polymer‐based adhesive hydrogels are presented.

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Adhesive, Stretchable, and Spatiotemporal Delivery Fibrous Hydrogels Harness Endogenous Neural Stem/Progenitor Cells for Spinal Cord Injury Repair

Cited by 57 publications

References 65 publications

Three-dimensional-printed collagen/chitosan/secretome derived from HUCMSCs scaffolds for efficient neural network reconstruction in canines with traumatic brain injury

Three-dimensional-printed collagen/chitosan/secretome derived from HUCMSCs scaffolds for efficient neural network reconstruction in canines with traumatic brain injury

Current treatments after spinal cord injury: Cell engineering, tissue engineering, and combined therapies

Natural polymer‐based adhesive hydrogel for biomedical applications

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