Adipocyte fatty acid mobilization in vivo: Effects of age and anatomical location

Abstract

The objectives of the present study were to determine if adipocyte triglyceride fatty acid (TGFA) mobilization in vivo varied among the different adipose tissue depots and whether these rates were affected by age. In order to accomplish these objectives, two groups of rats were studied. The first group initially weighed 84 +/- 1 and the second group 333 +/- 2. Both groups were placed on a semisynthetic diet containing 6% corn oil (w/w) and 14% triundecanoin (w/w) for a period of 4 wk. Triundecanoin contains an 11-carbon (C-11) fatty acid (undecanoic acid) that was used to label the adipocyte TGFA. At the end of the 4-wk feeding period, triundecanoin was removed from the diet and replaced with an equivalent amount of corn oil. At this time and at weekly intervals for the next 4 wk, 5 rats from each age group were killed for the determination of TGFA composition in isolated adipocytes from the epididymal (Epi), perirenal (PR), subcutaneous (SC) and mesenteric (M) adipose tissue depots. When the content of C-11 was expressed as mole percent of the total fatty acids, mobilization was significantly more rapid from the PR and M depots than in the other two depots in the young rats. In the older rats mobilization was significantly slower in all depots compared to the younger group. The rates of mobilization were not different between the depots in the older animals. Since fat cell size continued to increase throughout the duration of the study, part of the decrease in C-11 content can be accounted for by dilution by newly acquired TGFA.(ABSTRACT TRUNCATED AT 250 WORDS)

Characterization of adipocytes derived from fibro/adipogenic progenitors resident in human skeletal muscle

“…As adipogenic activities have been found to be age dependent, 30 , 31 FAP-As derived from donors <9 years of age were used to match ages of ASC references.…”

The Relationship between Perirenal Fat Thickness and Reduced Glomerular Filtration Rate in Patients with Type 2 Diabetes

“…Histologically, paranephric fat is a typical white adipose tissue depot, while perirenal fat mainly consists of dormant brown adipose tissue [ 22 ]. Besides, perirenal fat is more active in energy metabolism and adipokine secretion compared with typical visceral fat [ 17 , 23 ]. Renal sinus fat has been proved to increase in patients with prediabetes and diabetes, and negatively associated with estimated glomerular filtration rate (eGFR) in patients with type 2 diabetes mellitus (T2DM) [ 24 , 25 ].…”

Fat depot origin affects adipogenesis in primary cultured and cloned human preadipocytes

“…For several decades, it has been appreciated that fat tissue is regionally heterogeneous with respect to metabolic function (2,29,30). Fat cells isolated from different depots of rats and humans differ in size, responses to insulin and lipolytic agents, lipoprotein lipase release, lipid synthetic capacity, fatty acid incorporation, and other characteristics (2,6,11,14,18,23,36,37,47,53,56,58). These observations lead to the following question: Is interdepot variation solely a result of influences extrinsic to adipose cells (including their hormonal and paracrine microenvironment, local nutrient availability, innervation, and anatomic constraints), or do intrinsic differences in the innate characteristics of adipose cells also contribute?…”