Adipogenic Differentiation of Adipose-Derived Stem Cells

Yu, Gang; Floyd, Z. Elizabeth; Wu, Xiying; Hebert, Teddi; Halvorsen, Yuan-Di C.; Buehrer, Benjamin M.; Gimble, Jeffrey M.

doi:10.1007/978-1-61737-960-4_14

Cited by 61 publications

(54 citation statements)

References 10 publications

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“…It is largely known that hASCs have high potential to differentiate in adipocytes [4,5] under specific stimuli. In this scenarios, recent works reported the effects of dexamethasone on rat tendon stem cells differentiation in adipocytes and the molecular pathway underlying this process [6].…”

Section: Introductionmentioning

confidence: 99%

Hybrid Complexes of High and Low Molecular Weight Hyaluronans Highly Enhance HASCs Differentiation: Implication for Facial Bioremodelling

Stellavato

Noce

Corsuto

et al. 2017

Cell Physiol Biochem

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Background/Aims: Adipose-derived Stem Cells (ASCs) are used in Regenerative Medicine, including fat grafting, recovery from local tissue ischemia and scar remodeling. The aim of this study was to evaluate hyaluronan based gel effects on ASCs differentiation and proliferation. Methods: Comparative analyses using high (H) and low (L) molecular weight hyaluronans (HA), hyaluronan hybrid cooperative complexes (HCCs), and high and medium cross-linked hyaluronan based dermal fillers were performed. Human ASCs were characterized by flow cytometry using CD90, CD34, CD105, CD29, CD31, CD45 and CD14 markers. Then, cells were treated for 7, 14 and 21 days with hyaluronans. Adipogenic differentiation was evaluated using Oil red-O staining and expression of leptin, PPAR-γ, LPL and adiponectin using qRT-PCR. Adiponectin was analyzed by immunofluorescence, PPAR-γ and adiponectin were analyzed using western blotting. ELISA assays for adiponectin and leptin were performed. Results: HCCs highly affected ASCs differentiation by up-regulating adipogenic genes and related proteins, that were also secreted in the culture medium. H-HA and L-HA induced a lower level of ASCs differentiation. Conclusion: HCCs-based formulations clearly enhance adipogenic differentiation and proliferation, when compared with linear HA and cross-linked hyaluronans. Injection of HCCs in subdermal fat compartment may recruit and differentiate stem cells in adipocytes, and considerably improving fat tissue renewal.

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Section: Introductionmentioning

confidence: 99%

Hybrid Complexes of High and Low Molecular Weight Hyaluronans Highly Enhance HASCs Differentiation: Implication for Facial Bioremodelling

Stellavato

Noce

Corsuto

et al. 2017

Cell Physiol Biochem

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“…Cells from 3 donors were used, and representative data were shown. Human adipose stem cells were isolated, cultured, and differentiated as described (37 qPCR and Western Blotting. Total RNA from cultured cells or tissues was isolated using the TRIzol method (Invitrogen) combined with Qiagen RNAEasy minicolumns, according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Fat cells directly sense temperature to activate thermogenesis

Cohen

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

214

181

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Classic brown fat and inducible beige fat both dissipate chemical energy in the form of heat through the actions of mitochondrial uncoupling protein 1. This nonshivering thermogenesis is crucial for mammals as a defense against cold and obesity/diabetes. Cold is known to act indirectly through the sympathetic nervous systems and β-adrenergic signaling, but here we report that cool temperature (27–33 °C) can directly activate a thermogenic gene program in adipocytes in a cell-autonomous manner. White and beige fat cells respond to cool temperatures, but classic brown fat cells do not. Importantly, this activation in isolated cells is independent of the canonical cAMP/Protein Kinase A/cAMP response element-binding protein pathway downstream of the β-adrenergic receptors. These findings provide an unusual insight into the role of adipose tissues in thermoregulation, as well as an alternative way to target nonshivering thermogenesis for treatment of obesity and metabolic diseases.

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“…Spearman correlation coefficients (r) were calculated comparing mRNA expression of A2bAR to KLF4. C and D, human preadipocytes were isolated and differentiated to adipocytes for 10 days as described previously (24,25). We propose that A2bAR signaling, via a sustained elevation in KLF4, is an upstream determinant of preadipocyte fate.…”

Section: Discussionmentioning

confidence: 97%

“…The media were changed every 3 days. Human preadipocytes were isolated and differentiated to adipocytes for 10 days as described previously (24,25).…”

Section: Methodsmentioning

confidence: 99%