Adiponectin AS lncRNA inhibits adipogenesis by transferring from nucleus to cytoplasm and attenuating Adiponectin mRNA translation

Cai, Rui; Sun, Yunmei; Qimuge, Naren; Wang, Guoqiang; Wang, Yingqian; Chu, Guiyan; Yu, Taiyong; Yang, Gongshe; Pang, Wei

doi:10.1016/j.bbalip.2018.01.005

Cited by 80 publications

(70 citation statements)

References 52 publications

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“…The upregulation of the proadipogenic lncRNA NEAT1 was found to be essential for adipogenesis in human ASCs and is protected from degradation by miR‐140 in mouse ASCs (Gernapudi et al, ). In addition, the lncRNAs Gm15290, adipogenic differentiation induced noncoding RNA (ADINR), and adipocyte differentiation‐associated long noncoding RNA (ADNCR) all modulate adipogenesis via targeting the major adipogenic transcription factors PPARγ and cytosine–cytosine–adenosine–adenosine–thymidine (CCAAT)‐enhancer‐binding protein α (C/EBPα; Table ), while AdipoQ antisense lncRNA was unexpectedly found to inhibit not only the translation of adiponectin mRNA but also adipogenesis itself, possibly through as yet unidentified interactions with adipogenic factors (Cai et al, ).…”

Section: Overview Of Recently Published Work In Ascsmentioning

confidence: 99%

“…Similar to miRNAs, lncRNAs (>200 nucleotides) also regulate adipogenesis through various mechanisms, involving interactions with both mRNAs and miRNAs (Table 2) while AdipoQ antisense lncRNA was unexpectedly found to inhibit not only the translation of adiponectin mRNA but also adipogenesis itself, possibly through as yet unidentified interactions with adipogenic factors (Cai et al, 2018).…”

Section: Dna Methylationmentioning

confidence: 99%

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Adipocyte biology: It is time to upgrade to a new model

Gijsen

2018

Journal Cellular Physiology

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Globally, the obesity pandemic is profoundly affecting quality of life and economic productivity, but efforts to address this, especially on a pharmacological level, have generally proven unsuccessful to date, serving as a stark demonstration that our understanding of adipocyte biology and pathophysiology is incomplete. To deliver better insight into adipocyte function and obesity, we need improved adipocyte models with a high degree of fidelity in representing the in vivo state and with a diverse range of experimental applications. Adipocyte cell lines, especially 3T3-L1 cells, have been used extensively over many years, but these are limited in terms of relevance and versatility. In this review, I propose that primary adipose-derived stromal/stem cells (ASCs) present a superior model with which to study adipocyte biology ex vivo. In particular, ASCs afford us the opportunity to study adipocytes from different, functionally distinct, adipose depots and to investigate, by means of in vivo/ex vivo studies, the effects of many different physiological and pathophysiological factors, such as age, body weight, hormonal status, diet and nutraceuticals, as well as disease and pharmacological treatments, on the biology of adipocytes and their precursors. This study will give an overview of the characteristics of ASCs and published studies utilizing ASCs, to highlight the areas where our knowledge is lacking. More comprehensive studies in primary ASCs will contribute to an improved understanding of adipose tissue, in healthy and dysfunctional states, which will enhance our efforts to more successfully manage and treat obesity.

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Section: Overview Of Recently Published Work In Ascsmentioning

confidence: 99%

Section: Dna Methylationmentioning

confidence: 99%

Adipocyte biology: It is time to upgrade to a new model

Gijsen

2018

Journal Cellular Physiology

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“…Long non-coding RNAs (lncRNAs) are a type of non-coding RNA that are over 200 nt in length, and are involved in various biological processes [4,5], such as adipose deposition [6]. It has been reported that the differentiation of adipocytes is affected by the natural antisense transcript of adiponectin, which forms a duplex with sense mRNA to inhibit the translation of adiponectin [7]. Moreover, PU.1 AS lncRNA has also been found to promote adipogenic differentiation in porcine intramuscular adipocytes [8].…”

Section: Introductionmentioning

confidence: 99%

Screening of lncRNA Profiles During Intramuscular Adipogenic Differentiation Inlongissimus Dorsi and Semitendinosus Muscles in Pigs

Wang¹,

Peng²,

Xu³

et al. 2020

Preprint

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Background: Intramuscular fat content is an important factor that determines meat quality in pigs. In recent years, epigenetic regulation has increasingly studied the physiological model of intramuscular fat. Although long non-coding RNAs play essential roles in various biological processes, their role in intramuscular fat deposition in pigs remains largely unknown. Results: In this study, intramuscular preadipocytes in the longissimus dorsi and semitendinosus of Large White pigs were isolated and induced into adipogenic differentiation in vitro. High-throughput RNA-seq was then carried out to estimate the expression of lncRNAs at 0, 2, and 8 days post-differentiation. At this stage, 2135 lncRNAs, including 575 novel lncRNAs, were identified. lncRNAs are shorter, less expressed, and less conserved RNAs than protein-coding mRNAs. KEGG analysis showed that the differentially expressed lncRNAs were more common in pathways that are closely involved with adipogenesis and lipid metabolism. Of these, lnc_000368, a previously undescribed lncRNA, was found to gradually increase during the adipogenic process. qRT-PCR and a western blot revealed that the knockdown of lnc_000368 by siRNA significantly repressed the expression of adipogenic genes (PPARγ, aP2, CEBPβ) and lipolytic genes (ATGL and HSL). As a result, lipid accumulation in porcine intramuscular adipocytes was impaired by the silencing of lnc_000368, as indicated by Oil Red O staining. Conclusions: Overall, our study identified a genome-wide lncRNA profile related to porcine intramuscular fat deposition, and the results suggest that lnc_000368 is a potential target gene that might be targeted in pig breeding in the future.

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“…ADNCR (adipocyte differentiation‐associated long noncoding RNA) represses adipogenesis by functioning as a competing endogenous RNA . Recently, AdipoQ AS lncRNA and lnc‐U90926 were also reported to play inhibitory roles in adipogenesis . Although numerous lncRNAs are known as regulators of adipogenesis, knowledge of lncRNAs that selectively modulate adipose metabolism during CAC development remains limited …”

Section: Introductionmentioning

confidence: 99%

“…22 Recently, AdipoQ AS lncRNA and lnc-U90926 were also reported to play inhibitory roles in adipogenesis. 23,24 Although numerous lncRNAs are known as regulators of adipogenesis, knowledge of lncRNAs that selectively modulate adipose metabolism during CAC development remains limited. 25 In our study, we aimed to identify novel and functionally important lncRNAs involved in CAL using mouse models.…”

Section: Introductionmentioning

confidence: 99%

Cachexia‐related long noncoding RNA, CAAlnc1, suppresses adipogenesis by blocking the binding of HuR to adipogenic transcription factor mRNAs

Shen

Han

et al. 2019

Intl Journal of Cancer

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Cancer‐associated cachexia (CAC) is a devastating syndrome characterized by progressive losses of adipose tissue and skeletal muscle. CAC‐related adipose tissue loss (CAL) occurs early and is associated with a shorter survival time. To explore potential regulatory long noncoding RNAs (lncRNAs) of CAL, RNA microarrays were used to analyze the transcriptomes of white adipose tissue from CAC mice vs. control mice. A set of differentially expressed lncRNAs was identified, and among them was CAAlnc1, which suppressed adipogenesis of C3H10 cells as demonstrated by gain‐of‐function and loss‐of‐function experiments. RNA immunoprecipitation and pull‐down assays revealed Hu antigen R (HuR) was an important binding partner of CAAlnc1. The interaction between CAAlnc1 and HuR blocked the binding of HuR to adipogenic transcription factor mRNAs and further downregulated the expression of these transcription factors. This study generated a list of CAL‐related lncRNAs and provided details of a functional lncRNA which may play an important role in CAL.

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Adiponectin AS lncRNA inhibits adipogenesis by transferring from nucleus to cytoplasm and attenuating Adiponectin mRNA translation

Cited by 80 publications

References 52 publications

Adipocyte biology: It is time to upgrade to a new model

Adipocyte biology: It is time to upgrade to a new model

Screening of lncRNA Profiles During Intramuscular Adipogenic Differentiation Inlongissimus Dorsi and Semitendinosus Muscles in Pigs

Cachexia‐related long noncoding RNA, CAAlnc1, suppresses adipogenesis by blocking the binding of HuR to adipogenic transcription factor mRNAs

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Adiponectin AS lncRNA inhibits adipogenesis by transferring from nucleus to cytoplasm and attenuating Adiponectin mRNA translation

Cited by 80 publications

References 52 publications

Adipocyte biology: It is time to upgrade to a new model

Adipocyte biology: It is time to upgrade to a new model

Screening of lncRNA Profiles During Intramuscular Adipogenic Differentiation Inlongissimus Dorsi&nbsp;and&nbsp;Semitendinosus&nbsp;Muscles in Pigs

Cachexia‐related long noncoding RNA, CAAlnc1, suppresses adipogenesis by blocking the binding of HuR to adipogenic transcription factor mRNAs

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Screening of lncRNA Profiles During Intramuscular Adipogenic Differentiation Inlongissimus Dorsi and Semitendinosus Muscles in Pigs