Adipose-Derived Mesenchymal Stem Cells and Conditioned Medium Attenuate the Memory Retrieval Impairment During Sepsis in Rats

Akhondzadeh, Fariba; Kadkhodaee, Mehri; Seifi, Behjat; Ashabi, Ghorbangol; Kianian, Farzaneh; Abdolmohammadi, Kamal; Izad, Maryam; Adelipour, Maryam; Ranjbaran, Mina

doi:10.1007/s12035-020-01991-6

Cited by 15 publications

(10 citation statements)

References 53 publications

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“…ADMSCs and conditioned medium can attenuate sepsis injury in rats [ 19 ]. To explore whether ADMSCs can reduce sepsis injury through exosomes, we first isolated and purified ADMSCs from rat adipose tissue.…”

Section: Resultsmentioning

confidence: 99%

Exosomes from adipose-derived mesenchymal stem cells alleviate sepsis-induced lung injury in mice by inhibiting the secretion of IL-27 in macrophages

et al. 2022

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Acute lung injury (ALI) represents a frequent sepsis-induced inflammatory disorder. Mesenchymal stromal cells (MSCs) elicit anti-inflammatory effects in sepsis. This study investigated the mechanism of exosomes from adipose-derived MSCs (ADMSCs) in sepsis-induced ALI. The IL-27r−/− (WSX-1 knockout) or wild-type mouse model of sepsis was established by cecal ligation and puncture (CLP). The model mice and lipopolysaccharide (LPS)-induced macrophages were treated with ADMSC-exosomes. The content of Dil-labeled exosomes in pulmonary macrophages, macrophages CD68+ F4/80+ in whole lung tissues, and IL-27 content in macrophages were detected. The mRNA expression and protein level of IL27 subunits P28 and EBI3 in lung tissue and the levels of IL-6, TNF-α, and IL-1β were measured. The pulmonary edema, tissue injury, and pulmonary vascular leakage were measured. In vitro, macrophages internalized ADMSC-exosomes, and ADMSC-exosomes inhibited IL-27 secretion in LPS-induced macrophages. In vivo, IL-27 knockout attenuated CLP-induced ALI. ADMSC-exosomes suppressed macrophage aggregation in lung tissues and inhibited IL-27 secretion. ADMSC-exosomes decreased the contents of IL-6, TNF-α, and IL-1β, reduced pulmonary edema and pulmonary vascular leakage, and improved the survival rate of mice. Injection of recombinant IL-27 reversed the protective effect of ADMSC-exosomes on sepsis mice. Collectively, ADMSC-exosomes inhibited IL-27 secretion in macrophages and alleviated sepsis-induced ALI in mice.

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Section: Resultsmentioning

confidence: 99%

Exosomes from adipose-derived mesenchymal stem cells alleviate sepsis-induced lung injury in mice by inhibiting the secretion of IL-27 in macrophages

et al. 2022

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“…Phenotypic analysis confirms the expression of various MSCs-related surface markers. Most MSCs are positive for cluster of differentiation (CD)73 (5'nucleotidase) [ 45 – 51 ], CD90 (Thy1 membrane glycoprotein) [ 48 – 54 ], CD105 (endoglin) [ 32 , 50 , 51 , 55 – 57 ], CD44 (hyaluronan receptor) [ 48 , 49 , 58 , 59 ], and lack expressions for CD34 (hematopoietic progenitor cell antigen) [ 32 , 55 , 56 , 60 , 61 ], CD14 (myeloid cell-specific leucine-rich glycoprotein) [ 60 – 63 ], CD45 (protein tyrosine-phosphatase) [ 64 – 68 ], and HLA-DR (human leukocyte antigens class II DR) [ 62 , 68 , 69 ]. Individual markers include CD146 (S-endo1, melanoma cell adhesion molecule, Muc18, or glycerin) [ 65 – 67 , 70 ], CD29 (integrin β1) [ 45 – 47 , 52 , 53 , 64 – 66 ], CD49e (integrin α5), CD54 (intercellular adhesion molecule 1), CD106 (vascular cell adhesion molecule) [ 63 ], CD146 (melanoma cell adhesion molecule) [ 32 , 55 , 56 , 67 , 70 ], CD166 (activated leukocyte cell adhesion molecule) [ 63 , 67 ], CD271 (low-affinity nerve growth factor receptor) [ 32 , 46 , 47 , 55 , 56 , 65 – 67 ], SSEA-4 (stage-specific embryonic antigen-4) [ 45 ], Notch 1 (neurogenic locus notch homologue protein 1), HLA-ABC (human leukocyte antigens, histocompatibility complex class I molecules) [ 71 , 72 ], and Stro1 (stromal antigen 1) [ 68 …”

Section: Mscs and Mscs-exosomes: Cell Selection And Preparationmentioning

confidence: 99%

Mesenchymal stromal cell-based therapy for cartilage regeneration in knee osteoarthritis

Xiang

Zhu

et al. 2022

Stem Cell Res Ther

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Osteoarthritis, as a degenerative disease, is a common problem and results in high socioeconomic costs and rates of disability. The most commonly affected joint is the knee and characterized by progressive destruction of articular cartilage, loss of extracellular matrix, and progressive inflammation. Mesenchymal stromal cell (MSC)-based therapy has been explored as a new regenerative treatment for knee osteoarthritis in recent years. However, the detailed functions of MSC-based therapy and related mechanism, especially of cartilage regeneration, have not been explained. Hence, this review summarized how to choose, authenticate, and culture different origins of MSCs and derived exosomes. Moreover, clinical application and the latest mechanistical findings of MSC-based therapy in cartilage regeneration were also demonstrated.

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“…In experimental sepsis, mesenchymal stromal cells (MSC) mitigate BBB dysfunction and neuroinflammation, reduce astrogliosis, and lead to long-term improvements in cognition and anxiety-like behavior [ 83 ], as well as resulting in better memory retrieval and decreased sepsis scores at acute time points [ 84 ]. Treatment with statins [ 85 ], antidepressants [ 86 ], and resveratrol [ 87 ] reduces microglial activation and prevents long-term cognitive dysfunction [ 85 ] and attenuates cognitive and behavioral impairments [ 86 , 87 ].…”

Section: Sepsis-associated Encephalopathymentioning

confidence: 99%

Infectious disease-associated encephalopathies

et al. 2021

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Infectious diseases may affect brain function and cause encephalopathy even when the pathogen does not directly infect the central nervous system, known as infectious disease-associated encephalopathy. The systemic inflammatory process may result in neuroinflammation, with glial cell activation and increased levels of cytokines, reduced neurotrophic factors, blood–brain barrier dysfunction, neurotransmitter metabolism imbalances, and neurotoxicity, and behavioral and cognitive impairments often occur in the late course. Even though infectious disease-associated encephalopathies may cause devastating neurologic and cognitive deficits, the concept of infectious disease-associated encephalopathies is still under-investigated; knowledge of the underlying mechanisms, which may be distinct from those of encephalopathies of non-infectious cause, is still limited. In this review, we focus on the pathophysiology of encephalopathies associated with peripheral (sepsis, malaria, influenza, and COVID-19), emerging therapeutic strategies, and the role of neuroinflammation. Graphic abstract

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Adipose-Derived Mesenchymal Stem Cells and Conditioned Medium Attenuate the Memory Retrieval Impairment During Sepsis in Rats

Cited by 15 publications

References 53 publications

Exosomes from adipose-derived mesenchymal stem cells alleviate sepsis-induced lung injury in mice by inhibiting the secretion of IL-27 in macrophages

Exosomes from adipose-derived mesenchymal stem cells alleviate sepsis-induced lung injury in mice by inhibiting the secretion of IL-27 in macrophages

Mesenchymal stromal cell-based therapy for cartilage regeneration in knee osteoarthritis

Infectious disease-associated encephalopathies

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