Adipose-derived mesenchymal stem cells reduced transient cerebral ischemia injury by modulation of inflammatory factors and AMPK signaling

Ranjbaran, Mina; Vaali, Reyhaneh; Yaghoobi, Zahra; Sehati, Fardin; Jashn, Vida; Kolur, Sevda Mahdipour; Akhondzadeh, Fariba; Ashabi, Ghorbangol

doi:10.1016/j.bbr.2022.114001

Cited by 8 publications

(5 citation statements)

References 45 publications

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“…Recent research indicated that the positive effects on tissue repair following mesenchymal stem cell transplantation resulted from releasing soluble factors through the paracrine mechanism [ 65 ]. In our recent studies, MSCs transplantation via intraperitoneal injection have shown that MSCs may have beneficial therapeutic effects in septic shock and cerebral ischemia reperfusion, primarily through paracrine pathways [ 20 , 66 , 67 ]. Several studies, using animal model and transitional research, have demonstrated that MSCs could home to sites of injury, such as the ovary in PCOS.…”

Section: Discussionmentioning

confidence: 99%

The effect of adipose-derived mesenchymal stem cell transplantation on ovarian mitochondrial dysfunction in letrozole-induced polycystic ovary syndrome in rats: the role of PI3K-AKT signaling pathway

Abdi,

Ranjbaran,

Amidi

et al. 2024

J Ovarian Res

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Objective The present study aimed to elucidate how mesenchymal stem cells (MSCs) application could efficiently attenuate pathological changes of letrozole-induced poly cystic ovary syndrome (PCOS) by modulating mitochondrial dynamic via PI3K-AKT pathway. Methods Thirty-two female rats were randomly divided into four experimental groups: Sham, PCOS, PCOS + MSCs, and PCOS + MSCs + LY294002. The Sham group received 0.5% w/v carboxymethyl cellulose (CMC); the PCOS group received letrozole (1 mg/kg, daily) in 0.5% CMC for 21 days. Animals in the PCOS + MSCs group received 1 × 106 MSCs/rat (i.p,) on the 22th day of the study. In the PCOS + MSCs + LY294002 group, rats received LY294002 (PI3K-AKT inhibitor) 40 min before MSC transplantation. Mitochondrial dynamic gene expression, mitochondrial membrane potential (MMP), citrate synthase (CS) activity, oxidative stress, inflammation, ovarian histological parameters, serum hormone levels, homeostatic model assessment for insulin resistance (HOMA-IR), insulin and glucose concentrations, p-PI3K and p-AKT protein levels were evaluated at the end of the experiment. Results PCOS rats showed a significant disruption of mitochondrial dynamics and histological changes, lower MMP, CS, ovary super oxide dismutase (SOD) and estrogen level. They also had a notable rise in insulin and glucose concentrations, HOMA-IR, testosterone level, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels, ovarian malondialdehyde (MDA) content as well as a notable decrease in p-PI3K and p-AKT protein levels compared to the Sham group. In the PCOS + MSCs group, the transplantation of MSCs could improve the above parameters. Administration of LY294002 (PI3K-AKT pathway inhibitor) deteriorated mitochondrial dynamic markers, oxidative stress status, inflammation markers, hormonal levels, glucose, and insulin levels and follicular development compared to the PCOS + MSCs group. Conclusions This study demonstrated that the protective effects of MSC transplantation in regulating mitochondrial dynamics, promoting mitochondrial biogenesis, competing with redox status and inflammation response were mainly mediated through the PI3K-AKT pathway in the PCOS model.

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Section: Discussionmentioning

confidence: 99%

The effect of adipose-derived mesenchymal stem cell transplantation on ovarian mitochondrial dysfunction in letrozole-induced polycystic ovary syndrome in rats: the role of PI3K-AKT signaling pathway

Abdi,

Ranjbaran,

Amidi

et al. 2024

J Ovarian Res

Self Cite

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“…1,4), the lower the proportion of apoptotic cells, the more obvious the improvement of vertebral artery blood supply, so apoptosis may be a potential therapeutic target for CSA. Bax and Bcl-2 are often used to evaluate the extent of apoptosis [37,38]. During apoptosis, the role of BAX is to induce apoptosis, contrary to bax, Bcl-2 inhibits cell apoptosis by forming a dimer with Bax) [39,40].…”

Section: Discussionmentioning

confidence: 99%

Effects of Massage on Neuro-Vascular Regulation and Apoptosis in Rabbits with Cervical Spondylosis of the Vertebral Artery Type

Wang

Hui

Xiong

et al. 2023

Preprint

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Objective To explore the therapeutic mechanisms of massage for cervical spondylosis of vertebral artery type (CSA) from the effects of sympathetic neurotransmitter changes on vertebral artery blood flow and apoptosis. Methods Forty rabbits were randomly divided into a normal group, model group, electroacupuncture (EA) group, and massage group, with 10 rabbits in each group. The CSA rabbit model was established by neck injection of sclerosing agent in all groups except the normal group. In the EA group, the left “Fengchi” (GB 20) and the 3rd-5th cervical vertebrae (C3-5) “Jiaji” (EX-B2) were selected for EA treatment. In the massage group, pushing manipulation with one finger was performed at 0.5 cm to the left side of the C3-5 spinous process and the tip of the transverse process. The vertebral artery blood flow was detected by laser Doppler. The levels of serum neuropeptide Y (NPY) and norepinephrine (NE) were determined by ELISA. The pathological morphological changes of vertebral arteries were observed by HE staining. The apoptosis of vertebral arteries and cerebella were detected by Tunel assay. The protein expressions of CHOP, Bcl-2, and Bax in vertebral arteries and cerebella were detected by Western blot. Results Vertebral artery blood flow was significantly decreased in all rabbits after modeling. Massage increased vertebral artery blood flow, decreased serum levels of NPY and NE which secreted by sympathetic nerves, improved vertebral artery lumen narrowing, intimal thinning, and mesenteric smooth muscle cell alignment. Moreover, these effects were superior to that in the EA group. In addition, the massage group significantly reduced the apoptotic index, decreased the CHOP and Bcl-2 associated X protein (Bax) protein expressions, and increased the B-cell lymphoma-2 (Bcl-2) protein expression in vertebral arteries and cerebella relative to the model group. Conclusion The treatment of CSA has a neuro-vascular regulatory mechanism. Massage can decrease the release of sympathetic neurotransmitters that constrict blood vessels and mitigate apoptosis induced by excessive endoplasmic reticulum stress (ERS) due to sympathetic excitation, so as to improve vertebral artery blood flow and serve as a treatment for CSA.

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“…MSC therapy modulates BDNF/TrkB to protect neurons from ER stress-induced apoptosis after stroke [ 86 ]. MSCs can induce intracellular neuroprotective pathways in rats with stroke by stimulating Klotho-α/AMPK-α signal transduction, suppressing pro-inflammatory factors, and reducing hippocampal apoptosis [ 87 ].…”

Section: The Therapeutic Mechanism Of Mscs In Neurological Disordersmentioning

confidence: 99%

Therapeutic potential of mesenchymal stem cells for cerebral small vessel disease

Chen,

Huang,

et al. 2024

Regenerative Therapy

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Adipose-derived mesenchymal stem cells reduced transient cerebral ischemia injury by modulation of inflammatory factors and AMPK signaling

Cited by 8 publications

References 45 publications

The effect of adipose-derived mesenchymal stem cell transplantation on ovarian mitochondrial dysfunction in letrozole-induced polycystic ovary syndrome in rats: the role of PI3K-AKT signaling pathway

The effect of adipose-derived mesenchymal stem cell transplantation on ovarian mitochondrial dysfunction in letrozole-induced polycystic ovary syndrome in rats: the role of PI3K-AKT signaling pathway

Effects of Massage on Neuro-Vascular Regulation and Apoptosis in Rabbits with Cervical Spondylosis of the Vertebral Artery Type

Therapeutic potential of mesenchymal stem cells for cerebral small vessel disease

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